Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples

Journal of Medical Microbiology

Volumn 56, Issue 4, 2007, Pages 480-486

  Pacheco, Luis G C ^a   Pena, Roberta R ^a   Castro, Thiago L P ^a   Dorella, Fernanda A ^a   Bahia, Robson C ^b   Carminati, Renato ^b   Frota, Marcílio N L ^c   Oliveira, Sérgio C ^a   Meyer, Roberto ^b   Alves, Francisco S F ^d   Miyoshi, Anderson ^a   Azevedo, Vasco ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b FEDERAL UNIVERSITY OF BAHIA   (Brazil)

^c UNIVERSIDADE ESTADUAL VALE DO ACARAÚ   (Brazil)

^d EMBRAPA CERRADOS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CASEOUS LYMPHADENITIS; CONTROLLED STUDY; CORYNEBACTERIUM PSEUDOTUBERCULOSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA DETERMINATION; GENE AMPLIFICATION; GOAT; LYMPHADENITIS; MICROBIOLOGICAL EXAMINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLD GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RPOB GENE; SENSITIVITY AND SPECIFICITY; SHEEP; TAXONOMY;

ANIMALS; BASE SEQUENCE; CORYNEBACTERIUM INFECTIONS; CORYNEBACTERIUM PSEUDOTUBERCULOSIS; GOAT DISEASES; GOATS; LYMPH NODES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; SENSITIVITY AND SPECIFICITY; SHEEP; SHEEP DISEASES; SPECIES SPECIFICITY; SUPPURATION;

EID: 34247211719     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.46997-0     Document Type: Article

References (36)

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. J Mol Biol 215, 403-410.
2. Baird, G. (1997). Caseous lymphadenitis: an increasing cause for concern. Vet Rec 140, 611.
3. Biberstein, E. L., Knight, H. D. & Jang, S. (1971). Two biotypes of Corynebacterium pseudotuberculosis. Vet Rec 89, 691-692.
4. Brown, C. C., Olander, H. J., Biberstein, E. L. & Morse, S. M. (1986). Use of a toxoid vaccine to protect goats against intradermal challenge exposure to Corynebacterium pseudotuberculosis. Am J Vet Res 47, 1116-1119.
5. Brown, C. C., Olander, H. J. & Alves, S. F. (1987). Synergistic hemolysis-inhibition titers associated with caseous lymphadenitis in a slaughterhouse survey of goats and sheep in Northeastern Brazil. Can J Vet Res 51, 46-49.
6. Cetinkaya, B., Karahan, M., Atil, E., Kalin, R., De Baere, T. & Vaneechoutte, M. (2002). Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR. Vet Microbiol 88, 75-83.
7. Dercksen, D. P., Brinkhof, J. M. A., Dekker-Nooren, T., van Maanen, K., Bode, C. F., Baird, G. & Kamp, E. M. (2000). A comparison of four serological tests for the diagnosis of caseous lymphadenitis in sheep and goats. Vet Microbiol 75, 167-175.
8. DeWinter, L. M., Bernard, K. A. & Romney, M. G. (2005). Human clinical isolates of Corynebacterium diphtheriae and Corynebacterium ulcerans collected in Canada from 1999 to 2003 but not fitting reported criteria for cases of diphtheria. J Clin Microbiol 43, 3447-3449.
9. De Zoysa, A., Hawkey, P.M., Engler, K., George, R., Mann, G., Reilly, W., Taylor, D. & Efstratiou, A. (2005). Characterization of toxigenic Corynebacterium ulcerans strains isolated from humans and domestic cats in the United Kingdom. J Clin Microbiol 43, 4377-4381.
10. Dorella, F. A., Pacheco, L. G. C., Oliveira, S. C., Miyoshi, A. & Azevedo, V. (2006). Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet Res 37, 201-218.
11. Halbert, N. D., Reitzel, R. A., Martens, R. J. & Cohen, N. D. (2005). Evaluation of a multiplex polymerase chain reaction assay for simultaneous detection of Rhodococcus equi and the vapA gene. Am J Vet Res 66, 1380-1385.
12. Harrington, J. R., Golding, M. C., Martens, R. J., Halbert, N. D. & Cohen, M. D. (2005). Evaluation of real-time quantitative polymerase chain reaction assay for detection and quantitation of virulent Rhodococcus equi. Am J Vet Res 66, 755-761.
13. Honore-Bouakline, S., Vincensini, J. P., Giacuzzo, V., Lagrange, P. H. & Herrmann, J. L. (2003). Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction. J Clin Microbiol 41, 2323-2329.
14. Hoorfar, J., Malorny, B., Abdulmawjood, A., Cook, M., Wagner, M. & Fach, P. (2004). Practical considerations in design of internal amplification controls for diagnostic PCR assays. J Clin Microbiol 42, 1863-1868.
15. Khamis, A., Raoult, D. & La Scola, B. (2004). rpoB gene sequencing for identification of Corynebacterium species. J Clin Microbiol 42, 3925-3931.
16. Khamis, A., Raoult, D. & La Scola, B. (2005). Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium. J Clin Microbiol 43, 1934-1936.
17. Kuria, J. K. N., Mbuthia, P. G., Kang'ethe, E. K. & Wahome, R. G. (2001). Caseous lymphadenitis in goats: the pathogenesis, incubation period and serological response after experimental infection. Vet Res Commun 25, 89-97.
18. Kusukawa, M., Uemori, T., Asada, K. & Kato, I. (1990). Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction. Biotechniques 9, 66-68.
19. McMamara, P. J., Cuevas, W. A. & Songer, J. G. (1995). Toxic phospholipases D of Corynebacterium pseudotuberculosis, C. ulcerans and Arcanobacterium haemolyticum: cloning and sequence homology. Gene 156, 113-118.
20. Menzies, P. I. & Muckle, C. A. (1989). The use of a microagglutination assay for the detection of antibodies to Corynebacterium pseudotuberculosis in naturally infected sheep and goat flocks. Can J Vet Res 53, 313-318.
21. Menzies, P. I., Hwang, Y.-T. & Prescott, J. F. (2004). Comparison of an interferon-γ to a phospholipase D enzyme-linked immunosorbent assay for diagnosis of Corynebacterium pseudotuberculosis infection in experimentally infected goats. Vet Microbiol 100, 129-137.
22. O'Halloran, D. M. & Cafferkey, M. T. (2005). Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine. J Clin Microbiol 43, 3487-3490.
23. Paton, M. W., Walker, S. B., Rose, I. R. & Watt, G. F. (2003). Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccines in sheep flocks. Aust Vet J 81, 91-95.
24. Paule, B. J. A., Azevedo, V., Regis, L. F., Carminati, R., Bahia, C. R., Vale, V. L. C., Moura-Costa, L. F., Freire, S. M., Mascimento, I. & other authors (2003). Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting. Vet Immunol Immunopathol 96, 129-139.
25. Peel, M. M., Palmer, G. G., Stacpoole, A. M. & Kerr, T. G. (1997). Human lymphadenitis due to Corynebacterium pseudotuberculosis: report of ten cases from Australia and review. Clin Infect Dis 24, 185-191.
26. Persson, S. & Olsen, K. E. P. (2005). Multiplex PCR for identification of Campylobacter coli and Campylobacter jejuni from pure cultures and directly in stool samples. J Med Microbiol 54, 1043-1047.
27. Piontkowski, M. D. & Shivvers, D. W. (1998). Evaluation of a commercially available vaccine against Corynebacterium pseudotuberculosis for use in sheep. J Am Vet Med Assoc 212, 1765-1768.
28. Riegel, P., Ruimy, R., de Briel, D., Prevost, G., Jehl, F., Christen, R. & Monteil, H. (1995). Taxonomy of Corynebacterium diphtheriae and related taxa, with recognition of Corynebacterium ulcerans sp. nov. nom. rev. FEMS Microbiol Lett 126, 271-276.
29. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
30. Shigidi, M. T. (1979). A comparison of five serological tests for the diagnosis of experimental Corynebacterium ovis infection in sheep. Br Vet J 135, 172-177.
31. Smibert, R. M. & Krieg, M. R. (1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607-654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
32. Songer, J. G., Beckenbach, K., Marshall, M. M., Olson, G. B. & Kelley, L. (1988). Biochemical and genetic characterization of Corynebacterium pseudotuberculosis. Am J Vet Res 49, 223-226.
33. Spier, S. J., Leutenegger, C. M., Carroll, S. P., Loye, J. E., Pusterla, J. B., Carpenter, T. E., Mihalyi, J. E. & Madigan, J. E. (2004). Use of a real-time polymerase chain reaction-based fluorogenic 5′ nuclease assay to evaluate insect vectors of Corynebacterium pseudotuberculosis infections in horses. Am J Vet Res 65, 829-834.
34. Stanford, K., Brogden, K. A., McClelland, L. A., Kozub, G. C. & Audibert, F. (1998). The incidence of caseous lymphadenitis in Alberta sheep and assessment of impact by vaccination with commercial and experimental vaccines. Can J Vet Res 62, 38-43.
35. Wadowsky, R. M., Michaels, R. H., Libert, T., Kingsley, L. A. & Ehrlich, G. D. (1996). Multiplex PCR-based assay for detection of Bordetella pertussis in nasopharyngeal swab specimens. J Clin Microbiol 34, 2645-2649.
36. Williamson, L. H. (2001). Caseous lymphadenitis in small ruminants. Vet Clin North Am Food Anim Pract 17, 359-371.