Detection by flow cytometry of ESAT-6- and PPD-specific circulating CD4+ T lymphocytes as a diagnostic tool for tuberculosis

International Archives of Allergy and Immunology

Volumn 143, Issue 1, 2007, Pages 1-9

  Cosmi, Lorenzo ^a   Maggi, Laura ^a   Santarlasci, Veronica ^a   Liotta, Francesco ^a   Frosali, Francesca ^a   Angeli, Roberta ^a   Mazzetti, Marcello ^b   Vultaggio, Alessandra ^a   Matucci, Andrea ^a   Maggi, Enrico ^a   Romagnani, Sergio ^a   Annunziato, Francesco ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

^b Division of Infectious Diseases   (Italy)

Author keywords

Flow cytometry; Protein ESAT 6; Purified protein derivative; Tuberculosis

Indexed keywords

BCG VACCINE; EARLY SECRETORY ANTIGENIC TARGET 6; TUBERCULIN; TUMOR NECROSIS FACTOR ALPHA INHIBITOR;

ADULT; ANTIGEN SPECIFICITY; ARTICLE; BLOOD SAMPLING; CD4+ T LYMPHOCYTE; CLINICAL EVALUATION; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; FEMALE; FLOW CYTOMETRY; HUMAN; IMMUNOHISTOCHEMISTRY; IMMUNOLOGICAL PROCEDURES; IMMUNOSTIMULATION; MAJOR CLINICAL STUDY; MALE; MYCOBACTERIUM GORDONAE; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; RHEUMATOID ARTHRITIS; SENSITIVITY AND SPECIFICITY; TUBERCULIN TEST; TUBERCULOSIS;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; FEMALE; FLOW CYTOMETRY; HUMANS; MALE; TUBERCULIN; TUBERCULIN TEST; TUBERCULOSIS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 34247192521     PISSN: 10182438     EISSN: None     Source Type: Journal    
DOI: 10.1159/000098220     Document Type: Article

References (30)

1. Rook GA, Dheda K, Zumla A: Immune responses to tuberculosis in developing countries: implications for new vaccines. Nat Rev Immunol 2005;5:661-667.
2. Palme IB, Gudetta B, Bruchfeld J, Muhe L, Giesecke J: Impact of human immunodeficiency virus 1 infection on clinical presentation, treatment outcome and survival in a cohort of Ethiopian children with tuberculosis. Pediatr Infect Dis J 2002;2:1053-1061.
3. Magnani ZI, Confetti C, Besozzi G, Codecasa LR, Panina-Bordignon P, Lang R, Rossi GA, Pardi R, Burastero SE: Circulating, Mycobacterium tuberculosis -specific lymphocytes from PPD skin test-negative patients with tuberculosis do not secrete interferongamma (IFN-gamma) and lack the cutaneous lymphocyte antigen skin-selective homing receptor. Clin Exp Immunol 2000;119:99-106.
4. Ewer K, Deeks J, Alvarez L, Bryant G, Waller S, Andersen P, Monk P, Lalvani A: Comparison of T-cell-based assay with tuberculin skin test for diagnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak. Lancet 2003;361:1168-1173.
5. Huebner RE, Schein MF, Bass JB: The tuberculin skin test. Clin Infect Dis 1993;17:968-975.
6. Arend SM, Engelhard AC, Groot G, de Boer K, Andersen P, Ottenhoff TH, van Dissel JT: Tuberculin skin testing compared with T-cell responses to Mycobacterium tuberculosis-specific and non-specific antigens for detection of latent infection in persons with recent tuberculosis contact. Clin Diagn Lab Immunol 2001;8:1089-1096.
7. Lalvani A, Pathan AA, Durkan H, Wilkinson KA, Whelan A, Deeks JJ, Reece WH, Latif M, Pasvol G, Hill AV: Enhanced contact tracing and spatial tracking of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells. Lancet 2001;357:2017-2021.
8. Pathan AA, Wilkinson KA, Klenerman P, McShane H, Davidson RN, Pasvol G, Hill AVS, Lalvani A: Direct ex vivo analysis of antigen specific IFN-γ-secreting CD4 T cells in Mycobacterium tuberculosis infected individuals: associations with clinical disease state and effect of treatment. J Immunol 2001;167:5217-5225.
9. Tesfa L, Koch FW, Pankow W, Volk H-D, Kerne F. Confirmation of Mycobacterium tuberculosis infection by flow cytometry after ex vivo incubation of peripheral blood T cells with an ESAT-6-derived peptide pool. Cytometry 2004;60B:47-53.
10. Hughes AJ, Hutchinson P, Gooding T, Freezer NJ, Holdsworth SR, Johnson PD: Diagnosis of Mycobacterium tuberculosis infection using ESAT-6 and intracellular cytokine cytometry. Clin Exp Immunol 2005;142:132-139.
11. Lee E, Holzman RS: Evolution and current use of the tuberculin test. Clin Infect Dis 2002;34:365-370.
12. Suni M.A, Picker LJ, Maino VC: Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J Immunol Methods 1998;212:89-98.
13. Cosmi L, Santarlasci V, Angeli R, Liotta F, Maggi L, Frosali F, Rossi O, Falagiani P, Riva G, Romagnani S, Annunziato F: Sublingual immunotherapy with Dermatophagoides monomeric allergoid down-regulates allergen-specific immunoglobulin E and increases both interferon-γ- and interleukin-10- production. Clin Exp All 2006;36:261-272.
14. World health Organization. Global tuberculosis control: surveillance, planning, financing. Geneva, WHO/HTM/TB, 2004, p 331.
15. Sharma SK, Mohan A, Sharma A, Mitra DK: Miliary tuberculosis: new insights into an old disease. Lancet Infect Dis 2005;5:415-430.
16. Ulrichs T, Munk ME, Mollenkopf H, Behr-Perst S, Colangeli R, Gennaro ML, Kaufmann SH: Differential T cell responses to Mycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors. Eur J Immunol 1998;28:3949-3958.
17. Arend SM, van Meijgaarden KE, de Boer K, de Palou EC, van Soolingen D, Ottenhoff TH, van Dissel JT: Tuberculin skin testing and in vitro T cell responses to ESAT-6 and culture filtrate protein 10 after infection with Mycobacterium marinum or M. kansasii. J Infect Dis 2002;186:1797-1807.
18. Lalvani A, Pathan AA, McShane H, Wilkinson RJ, Latif M, Conlon CP, Pasvol G, Hill AV: Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells. Am J Respir Crit Care Med 2001;163:824-828.
19. Kern F, Bunde T, Faulhaber N, Kiecker F, Khatamzas E, Rudawski IM, Pruss A, Gratama JW, Volkmer-Engert R, Ewert R, Reinke P, Volk HD, Picker LJ: Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals. J Infect Dis 2002;185:1709-1716.
20. Keane J, Gershon S, Wise RP, Mirabile-Levens E, Kasznica J, Schwieterman WD, Siegel JN, Braun M.M: Tuberculosis associated with Infliximab, a tumor necrosis factor α-neutralizing agent. N Engl J Med 2001;345:1098-1104.
21. Frentsch M, Arbach O, Kirchhoff D, Moewes B, Worm M, Rothe M, Scheffold A, Thiel A: Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat Med 2005;11:1118-1124.
22. Boussiotis VA, Tsai EY, Yunis EJ, Thim S, Delgado JC, Dascher CC, Berezovskaya A, Rousset D, Reynes J.M, Goldfeld AE: IL-10-producing T cells suppress immune responses in anergic tuberculosis patients. J Clin Invest 2000;105:1317-1325.
23. Guyot-Revol V, Innes JA, Hackforth S, Hinks T, Lalvani A: Regulatory T cells are expanded in blood and disease sites in tuberculosis patients. Am J Respir Crit Care Med 2006;173:803-810.
24. American Thoracic Society, Centers for Disease Control: The tuberculin skin test. Am Rev Respir Dis 1981;124:356-363.
25. Keane J: TNF-blocking agents and tuberculosis: new drugs illuminate an old topic. Rheumatology 2005;44:714-720.
26. Mohan AK, Cote TR, Block JA, Manadan AM, Siegel JN, Braun MM: Tuberculosis following the use of etanercept, a tumour necrosis factor inhibitor. Clin Infect Dis 2004;39:295-299.
27. Wolfe F, Michaud K, Anderson J, Urbansky K: Tuberculosis infection in patients with rheumatoid arthritis and the effect of infliximab therapy. Arthritis Rheum 2004;50:372-379.
28. Ormerod LP, Milburn HJ, Gillespie S, Ledingham J, Rampton D: BTS recommendations for assessing risk, and for managing M. tuberculosis infection and disease in patients due to start anti-TNF-alpha treatment. Thorax 2005;60:800-805.
29. Joint Committee on Vaccination and Immunisation: Tuberculin Testing in Vaccination and Immunisation. London, HMSO, 1996, chapt 32.
30. Wilkinson KA, Kon MA, Newton SM, Meintjes G, Davidson MN, Pasvol G, Wilkinson RJ: Effect of treatment of latent tuberculosis infection on the T cell response to Mycobacterium tuberculosis antigens. J Infect Dis 2006;193:354-359.