A new method of purification of proteasome substrates reveals polyubiquitination of 20 S proteasome subunits

Journal of Biological Chemistry

Volumn 282, Issue 8, 2007, Pages 5302-5309

  Ventadour, Sophie ^a   Jarzaguet, Marianne ^a   Wing, Simon S ^c   Chambon, Christophe ^b   Combaret, Lydie ^a   Béchet, Daniel ^a   Attaix, Didier ^a   Taillandier, Daniel ^a  

^a INRA and University of Auvergne   (France)

^b CEMAGREF   (France)

^c MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY MATRIX; CELL LYSATE; POLYUBIQUITIN (UB); PROTEIN PROTEIN INTERACTIONS;

BIOACTIVITY; MASS SPECTROMETRY; MOLECULAR INTERACTIONS; MOLECULAR RECOGNITION; PURIFICATION; SUGARS; UNSATURATED COMPOUNDS;

PROTEINS;

20 S PROTEASOME; 26 S PROTEASOME; ANTIBODY; PROTEASOME; PROTEASOME INHIBITOR; PROTEIN SUBUNIT; UBIQUITIN PROTEIN LIGASE; UBIQUITIN SPECIFIC PEPTIDASE 2; UNCLASSIFIED DRUG; ATP DEPENDENT 26S PROTEASE; POLYUBIQUITIN;

ARTICLE; CATALYSIS; CELL LYSATE; ENZYME ACTIVE SITE; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME RELEASE; MASS SPECTROMETRY; MOLECULAR WEIGHT; MYOBLAST; NONHUMAN; POLYUBIQUITINATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; REGULATORY MECHANISM; SUCROSE GRADIENT; TECHNIQUE; TWO DIMENSIONAL GEL ELECTROPHORESIS; UBIQUITINATION; WESTERN BLOTTING; ANIMAL; CHEMISTRY; DROSOPHILA; ENZYME SPECIFICITY; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; PHYSIOLOGY; PROTEIN PROCESSING;

ANIMALS; DROSOPHILA; MICE; POLYUBIQUITIN; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SUBUNITS; SUBSTRATE SPECIFICITY;

EID: 34247166669     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M610005200     Document Type: Article

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