Membrane topology of aspartate:alanine antiporter AspT from Comamonas testosteroni

Journal of Biochemistry

Volumn 141, Issue 1, 2007, Pages 85-91

  Fujiki, Takashi ^a   Nanatani, Kei ^a   Nishitani, Kei ^a   Yagi, Kyoko ^a   Ohnishi, Fumito ^a   Yoneyama, Hiroshi ^a   Uchida, Takafumi ^a   Nakajima, Tasuku ^a   Abea, Keietsu ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Aspartate:alanine antiporter; Bacteria; Bioenergetics; Fusion methods; Membrane topology

Indexed keywords

ALANINE; AMINO ACID; ANTIPORTER; ASPARTIC ACID; HYBRID PROTEIN;

ARTICLE; ASPT GENE; BACTERIAL GENE; BACTERIAL GENETICS; BIOENERGY; COMAMONAS TESTOSTERONI; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENE ORDER; GENETIC VARIABILITY; LACTIC ACID BACTERIUM; MEMBRANE STRUCTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PROTEOLIPOSOME; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; TETRAGENOCOCCUS HALOPHILUS; WESTERN BLOTTING;

ALANINE; AMINO ACID SEQUENCE; ANTIPORTERS; ASPARTIC ACID; CELL MEMBRANE; CLONING, MOLECULAR; COMAMONAS TESTOSTERONI; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS;

COMAMONAS TESTOSTERONI; TETRAGENOCOCCUS HALOPHILUS;

EID: 34147222194     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvm010     Document Type: Article

References (27)

1. Mardashev, S.R. and Gladkova, V.N. (1948) Biokhimiya 13, 315
2. Meister, A., Sober, H.J., and Tice, S.V. (1951) Determination of aspartic and glutamic acids by enzymatic decarboxylation. J. Biol. Chem. 189, 591-595
3. Nishimura, J.S., Manning, J.M., and Meister, A. (1962) Studies on the mechanism of activation acid beta-decarboxylase by alpha-keto acids and pyridoxal 5′-phosphate. Biochemistry 1, 442-447
4. Cattaneo-Lacombe, J., Senez, J.C., and Beaumont, P. (1958) Purification of 4-aspartic acid decarboxylase of Desulfovibrio desulfuricans. Biochim. Biophys. Acta. 30, 458-465
5. Senez, J.C. and Cattaneo-Lacombe, J. (1956) C. R. Hebd. Seances. Acad. Sci. 242, 941-943
6. Crawford, L.V. (1958) Studies on the aspartic decarboxylase of Nocardia globerula. Biochem. J. 68, 221-225
7. Cooksey, K.E. and Rainbow, C. (1962) Metabolic patterns in acetic acid bacteria. J. Gen. Microbiol. 27, 135-142
8. Wilson, E.M. (1963) Crystalline L-aspartate 4-carboxy-lyase. Biochim. Biophys. Acta. 67, 345-348
9. Wilson, E.M. and Kornberg, H.L. (1963) Properties of crystalline L-Aspartate 4-carboxy-lase from Achromobacter sp. Biochem. J. 88, 578-587
10. Novogrodsky, A. and Meister, A. (1964) Control of Aspartate beta-decarboxylase activity by transamination,. J. Biol. Chem. 239, 879-888
11. Novogrodsky, A., Nishimura, J.S., and Meister, A. (1963) Transamination and beta-decarboxylation of aspartate catalyzed by the same pyridoxal phosphate-enzyme. J. Biol. Chem. 238, 1903-1905
12. Chibata, I., Kakimoto, T., and Kato, J. (1965) Enzymatic production of L-alanine by Pseudomonas dacunhae. Appl. Microbiol. 13, 638-645
13. Abe, K., Hayashi, H., and Maloney, P.C. (1996) Exchange of aspartate and alanine. J. Biol. Chem 271, 3079-3084
14. Abe, K., Ohnishi, F., Yagi, K., Nakajima, T., Higuchi, T., Sano, M., Machida, M., Sarker, R.I., and Maloney, P.C. (2002) Plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reaction. J. Bacteriol. 184, 2906-2913
15. Nanatani, K., Ohonishi, F., Yoneyama, H., Nakajima, T., and Abe, K. (2005) Membrane topology of the electrogenic aspartate-alanine antiporter AspT of Tetragenococcus halophilus. Biochem. Biophys. Res. Commun. 328, 20-26
16. Shibatani, T., Kakimoto, T., and Chibata, I. (1979) Stimulation of L-aspartate beta-decarboxylase formation by L-glutamate in Pseudomonas dacunhae and improved production of L-alanine. Appl. Environ. Microbiol. 38, 359-364
17. Eisenberg, D., Schwarz, E., Komaromy, M., and Wall, R. (1984) Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179, 125-142
18. Guan, L., Ehrmann, M., Yoneyama, H., and Nakae, T. (1999) Membrane topology of the xenobiotic-exporting subunit, MexB, of the MexA, B-OprM extrusion pump in Pseudomonas aeruginosa. J. Biol. Chem. 274, 10517-10522
19. Schaffner, W. and Weissmann, C. (1973) A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal. Biochem. 56, 502-514
20. Rozzell, J.D. Method and compositions for the production of L-alanine and derivatives thereof. U.S. Patent. 5,019,509.
21. Hirokawa, T., Boon-Chieng, S., and Mitaku, S. (1998) SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14, 378-379
22. Krogh, A., Larsson, B., von Heijne, G., and Sonnhammer, E.L. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305, 567-580
23. Sonnhammer, E.L., von Heijne, G., and Krogh, A. (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. Proc. Int. Conf. Intell. Syst. Mol. Biol. 6, 175-182
24. Hofman, K. and Stoffel, W. (1993) TMbase: a database of membrane spanning protein segments. Biol. Chem. Hoppe-Seyler 374, 166
25. von Heijne, G. (1989) Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues. Nature 341, 456-458
26. Andersson, H., Bakker, E., and von Heijne, G. (1992) Different positively charged amino acids have similar effects on the topology of a polytopic transmembrane protein in Escherichia coli. J. Biol. Chem. 267, 1491-1495
27. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410