Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases

Journal of Invertebrate Pathology

Volumn 95, Issue 1, 2007, Pages 48-59

  Qazi, Sohail S ^a   Khachatourians, George G ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Biological control; Electrophoretograms; Entomopathogenic fungi; Isoforms; Isozymes; Metalloproteases; Metarhizium anisopliae; Zymography

Indexed keywords

GELATINASE; ISOENZYME; METALLOPROTEINASE; WATER;

ANIMAL; ARTICLE; ENZYMOLOGY; FUNGUS SPORE; METABOLISM; METARHIZIUM; PATHOGENICITY;

ANIMALS; GELATINASES; ISOENZYMES; METALLOPROTEASES; METARHIZIUM; SPORES, FUNGAL; WATER;

BACTERIA (MICROORGANISMS); FUNGI; METARHIZIUM ANISOPLIAE;

EID: 34147162767     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2006.12.002     Document Type: Article

References (34)

1. Beynon R.J., and Salvesen G. Commercially available protease inhibitors. In: Beynon R.J., and Bond J.S. (Eds). Proteolytic Enzymes: A practical approach (2001), Oxford University Press, New York, Oxford 317-331
2. Bidochka M.J., and Khachatourians G.G. Purification and properties of an extracellular protease produced by the entomopathogenic fungus Beauveria bassiana. Appl. Environ. Microbiol. 53 (1987) 1679-1684
3. Bidochka M.J., and Khachatourians G.G. Regulation of extracellular protease in the entomopathogenic fungus Beauveria bassiana. Exp. Mycol. 12 (1988) 161-168
4. Boucias D.G., and Pendland J.C. Attachment of mycopathogens to cuticle: the initial event of mycoses in arthropod hosts. In: Cole G.T., and Hoch H.C. (Eds). The Fungal Spore and Disease Initiation in Plants and Animals (1991), Plenum press, New York 101-124
5. Brey P.T., Latge J.P., and Prevost M.C. Integumental penetration of the pea aphid, Acyrthosiphon pisum, by Conidiobolous obscurus. J. Invertebr. Pathol. 48 (1986) 34-41
6. Clarkson J.M., and Charnley A.K. New insights into the mechanisms of fungal pathogenesis in insects. Trends Microbiol. 4 (1996) 197-203
7. David W.A.L. The physiology of the insect integument in relation to the invasion of pathogens. In: Beament S.W.L., and Treherne S.E. (Eds). Insects and Physiology (1967), Oliver and Boyd, Edinburg 17-35
8. Dillon R.J., and Charnley A.K. A technique for accelerating and synchronizing germination of conidia of the entomopathogenic fungus Metarhizium anisopliae. Arch. Microbiol. 142 (1985) 204-206
9. Dillon R.J., and Charnley A.K. Initiation of germination in conidia of the entomopathogenic fungus Metarhizium anisopliae. Mycol. Res. 94 (1990) 299-304
10. Frobius A.C., Kanost M.R., Peter G., and Vilcinskas A. Isolation and characterization of novel inducible serine protease inhibitors from larval hemolymph of the greater wax moth Galleria mellonella. Eur. J. Biochem. 267 (2000) 2046-2053
11. Goettel M., St. Leger R.J., Rizzo R.J., Staples R.C., and Roberts D.W. Ultrastructural localization of a cuticle degrading protease produced by the entomopathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexta) cuticle. J. Gen. Microbiol. 135 (1989) 2233-2239
12. Hames B.D. One dimensional polyacrylamide gel electrophoresis. In: Hames B.D., and Rickwood D. (Eds). Gel Electrophoresis of Proteins: A Practical Approach (1985), IRL Press, Oxford and Washington, DC 1-147
13. Khachatourians, G.G., Qazi, S.S., Reid, W.R., 2007. Biochemistry and molecular biology of entomopathogenic fungi. In: Brakhage, A.A., Zipfer, P.F. (Eds.), The Mycota VI Human and Animal Relationships. Springer-Verlag, Berlin Heidelberg, Germany, pp. 1-42, in press.
14. Khachatourians G.G. Physiology and genetics of entomopathogenic fungi. In: Arora D.K., Ajello L., and Mukerji K.G. (Eds). Handbook of Applied Mycology. Humans, Animals, and Insects vol. 2 (1991), Marcel Dekker, New York 613-661
15. Khachatourians G.G. Biochemistry and molecular biology of entomopathogenic fungi. In: Howard D.H., and Miller J.D. (Eds). The Mycota VI Human and Animal Relationships (1996), Springer-Verlag, Berlin Heidelberg, Germany 331-363
16. Kobayashi T., Hakamada Y., Hitomi J., Koike K., and Ito S. Purification of alkaline proteases from a Bacillus strain and their possible interrelationship. Appl. Microbiol. Biotechnol. 45 (1996) 63-71
17. Lantz M.S., and Ciborowski P. Zymographic techniques for detection and characterization of microbial proteases. Methods Enzymol. 35 (1994) 563-593
18. Mellon J.E., and Cotty P.J. Purification and partial characterization of an elastinolytic proteinase from Aspergillus flavus filtrates. Appl. Microbiol. Biotechnol. 46 (1996) 138-142
19. Persson M., Hall L., and Soderhall K. Comparison of peptidase activity in some fungi pathogenic to arthropods. J. Invertebr. Pathol. 44 (1984) 342-348
20. Powers J.C., and Harper J.W. Protease inhibitors. In: Barrett S.J., and Salvesen C. (Eds) (1986), Elsevier, Amsterdam 219-289
21. Reisfeld R.A., Lewis V.J., and Williams D.E. Disc electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature 195 (1962) 281
22. Schmit J.C., and Brody S. Biochemical genetics of Neurospora crassa conidial germination. Bacteriol. Rev. 40 (1976) 2-41
23. Small C.-L.N., and Bidochka M.J. Up-regulation of Pr1, a subtilisin-like protease, during conidiation in the insect pathogen Metarhizium anisopliae. Mycol. Res. 109 (2005) 307-313
24. St. Leger R.J., Joshi L., and Roberts D.W. Ambient pH is a major determinant in the expression of cuticle-degrading enzymes and hydrophobin by Metarhizium anisopliae. Appl. Environ. Microbiol. 64 (1998) 709-713
25. St. Leger R.J. The role of cuticle-degrading proteases in fungal pathogens of insects. Can. J. Bot. 73 Suppl. 1 (1995) S1119-S1125
26. St. Leger R.J., Bidochka M.J., and Roberts D.W. Isoforms of the cuticle-degrading Pr1 proteinase and production of a metalloproteinase by Metarhizium. anisopliae. Arch. Biochem. Biophys. 313 (1994) 1-7
27. St. Leger R.J., Cooper R.M., and Charnley A.K. Cuticle degrading enzymes of entomopathogenic fungi: synthesis in culture on cuticle. J. Invertebr. Pathol. 48 (1986) 85-95
28. St. Leger R.J., Cooper R.M., and Charnley A.K. Production of cuticle-degrading enzymes by the entomopathogen M. anisopliae during the infection of cuticles from Calliphora vomitoria and Manduca sexta. J. Gen. Microbiol. 133 (1987) 1371-1382
29. St. Leger R.J., Goettel M., Roberts D.W., and Staples R.C. Prepenetration events during the infection of host cuticle by Metarhizium anisopliae. J. Invertebr. Pathol. 58 (1991) 168-179
30. Tong Y., and Kanost M.R. Manduca sexta Serpin-4 and Serpin-5 inhibit the prophenol oxidase activation pathway. J. Biol. Chem. 280 (2005) 14923-14931
31. Uribe, V.D., 2004. Mitochondrial genomic diversity and respiratory physiology in the entomopathogenic fungus Beauveria bassiana. Ph.D. thesis. University of Saskatchewan, Saskatoon, Canada.
32. Urtiz B.E., and Rice W.C. Purification and characterization of a novel extracellular protease from Beauveria bassiana. Mycol. Res. 104 (2000) 180-186
33. Yoshida S., Yamashita M., Yonehara S., and Eguchi M. Properties of fungal protease inhibitors from the integument and haemolymph of the silkworm and effect of an inhibitor on the fungal growth. Comp. Biochem. Physiol. (Part B-Biochem. Mol. Biol.) 95 (1990) 559-564
34. Zacharuk R.Y. Fine structure of the fungus Metarhizium anisopliae infecting three species of larval Elateridae (Coleoptera). III. Penetration of the host integument. J. Invertebr. Pathol. 15 (1970) 372-396