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Volumn 18, Issue 2, 2007, Pages 115-120

GM maize from site-specific recombination technology, what next?

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENES; GENETIC ENGINEERING; PLANTS (BOTANY);

EID: 34147154916     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2007.02.004     Document Type: Review
Times cited : (65)

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    • A novel approach to plastid transformation utilizes the phiC31 phage integrase
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    • Thomson J.G., and Ow D.W. Site-specific recombination systems for the genetic manipulation of eukaryotic genomes. Genesis 44 (2006) 465-476. Describes testing in fission yeast for site-specific recombination by three Int-att and four deletion systems that could potentially be used in plants. Given that commercial use of the most familiar site-specific recombination systems (e.g. Cre-lox and FLP-FRT) is under the control of a few companies, publicly developed integration and deletion systems provide welcome alternatives.
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    • Mlynárová L., Conner A.J., and Nap J.-P. Directed microspore-specific recombination of transgenic alleles to prevent pollen-mediated transmission of transgenes. Plant Biotech J 4 (2006) 445-452. Demonstrated Cre-mediated pollen-specific excision of transgenic DNA including the cre gene, which substantiates the view that site-specific excision can help control gene flow. However, the transgenic DNA, being absent in pollen, can only be maintained in a hemizygous state. Further development is needed to incorporate a method to repress Cre activity to yield homozygous transgenic lines for seed production.
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    • •], this method will not be suitable for use with non-vegetatively propagated crops until homozygous transgenic lines can be maintained.
    • •], this method will not be suitable for use with non-vegetatively propagated crops until homozygous transgenic lines can be maintained.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.