Characterization of the signaling domain of the NO-responsive regulator NorR from Ralstonia eutropha H16 by site-directed mutagenesis

Journal of Bacteriology

Volumn 189, Issue 7, 2007, Pages 2743-2749

  Klink, Andrea ^a   Elsner, Bettina ^a   Strube, Katja ^a   Cramm, Rainer ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

NITRIC OXIDE; NITRIC OXIDE REDUCTASE; PROTEIN NORA; PROTEIN NORB; PROTEIN NORR; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING COMPETITION; CISTRON; MEMBRANE BINDING; NONHUMAN; OPERON; PRIORITY JOURNAL; RALSTONIA EUTROPHA H16; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION REGULATION; WAUTERSIA EUTROPHA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BASE SEQUENCE; CUPRIAVIDUS NECATOR; DNA PRIMERS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SPECTROPHOTOMETRY; TRANS-ACTIVATORS;

CUPRIAVIDUS NECATOR;

EID: 34147133176     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01865-06     Document Type: Article

References (32)

1. Allemandou, F., J. Nussberger, H. R. Brunner, and N. Brakch. 2003. Rapid site-directed mutagenesis using two-PCR-generated DNA fragments reproducing the plasmid template. J. Biomed. Biotechnol. 2003:202-207.
2. Bodenmiller, D. M., and S. Spiro. 2006. The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator. J. Bacteriol. 188:874-881.
3. Busch, A., A. Pohlmann, B. Friedrich, and R. Cramm. 2004. A DNA region recognized by the nitric oxide-responsive transcriptional activator NorR is conserved in β- and γ-proteobacteria. J. Bacteriol. 186:7980-7987.
4. 2+ oxidase with metal coordination sites for small molecules and substrate. J. Biol. Chcm. 264:21677-21681.
5. Clay, M. D., C. A. Cosper, F. E. Jenney, Jr., M. W. Adams, and M. K. Johnson. 2003. Nitric oxide binding at the mononuclear active site of reduced Pyrococcus furiosus superoxide reductase. Proc. Natl. Acad. Sci. USA 100:3796-3801.
6. Corker, H., and R. K. Poole. 2003. Nitric oxide formation by Escherichia coli. Dependence on nitrite reductase, the NO-sensing regulator Fnr, and flavohemoglobin Hmp. J. Biol. Chem. 278:31584-31592.
7. Coufal, D. E., P. Tavares, A. S. Pereira, B. H. Hyunh, and S. J. Lippard. 1999. Reactions of nitric oxide with the reduced non-heme diiron center of the soluble methane monooxygenase hydroxylase. Biochemistry 38:4504-4513.
8. Cramm, R., A. Büsch, and K. Strube. 2006. NO-dependent transcriptional activation of gene expression in Ralstonia eutropha H16. Biochem. Soc. Trans. 34:182-184.
9. Gramm, R., R. A. Siddiqui, and B. Friedrich. 1997. Two isofunctional nitric oxide reductases in Alcaligenes eutrophus H16. J. Bacteriol. 179:6769-6777.
10. D'Autreaux, B., N. P. Tucker, R. Dixon, and S. Spiro. 2005. A non-haem iron centre in the transcription factor NorR senses nitric oxide. Nature 437:769-772.
11. Frey, M., L. Sieker, F. Payan, R. Haser, M. Bruschi, G. Pepe, and J. LeGall. 1987. Rubredoxin from Desulfovibrio gigas. A molecular model of the oxidized form at 1.4 Å resolution. J. Mol. Biol. 197:525-541.
12. Hennessy, D. J., G. R. Reid, F. E. Smith, and S. L. Thompson. 1984. Ferene: a new spectrophotometric reagent for iron. Can. J. Chem. 62:721-724.
13. Holm, R. H., P. Kennepohl, and E. I. Solomon. 1996. Structural and functional aspects of metal sites in biology. Chem. Rev. 96:2239-2314.
14. Hubner, P., J. C. Willison, P. M. Vignais, and T. A. Bickle. 1991. Expression of regulatory nif genes in Rhodobacter capsulatus. J. Bacteriol. 173:2993-2999.
15. Hutchings, M. I, N. Mandhana, and S. Spiro. 2002. The NorR protein of Escherichia coli activates expression of the flavorubredoxin gene norV in response to reactive nitrogen species. J. Bacteriol. 184:4640-4643.
16. Karlin, S., Z. Y. Zhu, and K. D. Karlin. 1997. The extended environment of mononuclear metal centers in protein structures. Proc. Natl. Acad. Sci. USA 94:14225-14230.
17. Lenz, O., E. Schwartz, J. Dernedde, M. Eitinger, and B. Friedrich. 1994. The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation. J. Bacteriol. 176:4385-4393.
18. 17a.Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
19. 2 activation mechanism. J. Biol. Inorg. Chem. 9:818-827.
20. Marx, C. J., and M. E. Lidström. 2001. Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Microbiology 147:2065-2075.
21. Nagashima, S., M. Nakasako, N. Dohmae, M. Tsujimura, K. Takio, M. Odaka, M. Yohda, N. Kamiya, and I. Endo. 1998. Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms. Nat. Struct. Biol. 5:347-351.
22. Pohlmann, A., R. Gramm, K. Schmelz, and B. Friedrich. 2000. A novel NO-responding regulator controls the reduction of nitric oxide in Ralstonia eutropha. Mol. Microbiol. 38:626-638.
23. Rodionov, D. A., I. L. Dubchak, A. P. Arkin, E. J. Alm, and M. S. Gelfand. 2005. Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks. PLoS Comput. Biol. 1:e55.
24. Schlegel, H. G., H. Kaltwasser, and G. Gottschalk. 1961. A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies. Arch. Mikrobiol. 38:209-222.
25. Simon, R., U. B. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1:784-791.
26. 54-dependent transcriptional activators. J. Bacteriol. 185:1757-1767.
27. Studier, F. W., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
28. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
29. Wanat, A., T. Schneppensieper, G. Stochel, R. van Eldik, E. Bill, and K. Wieghardt. 2002. Kinetics, mechanism, and spectroscopy of the reversible binding of nitric oxide to aquated iron(II). An undergraduate text book reaction revisited. Inorg. Chem. 41:4-10.
30. Weihs, V., K. Schmidt, B. Schneider, and B. Friedrich. 1989. The formation of an oxygen-binding flavohemoprotein from Alcaligenes eutrophus is plasmid-determined. Arch. Microbiol. 151:546-550.
31. Zumft, W. G. 1997. Cell biology and molecular basis of denitrification. Microbiol. Mol. Biol. Rev. 61:533-616.
32. Zumft, W. G. 2002. Nitric oxide signaling and NO dependent transcriptional control in bacterial denitrification by members of the FNR-CRP regulator family. J. Mol. Microbiol. Biotechnol. 4:277-286.