A Buoyancy-Driven Compact Thermocycler for Rapid PCR

Clinics in Laboratory Medicine

Volumn 27, Issue 1, 2007, Pages 215-223

  Agrawal, Nitin ^a   Ugaz, Victor M ^b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b Texas AandM University   (United States)

Author keywords

convective flow; DNA; PCR; thermocycler; thermosyphon

Indexed keywords

AUTOMATION; BIOCHEMISTRY; CHEMICAL REACTION KINETICS; DENATURATION; HEAT TRANSFER; LIQUID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVIEW; THERMODYNAMICS;

AUTOMATION; DNA; HEAT; INFLUENZA A VIRUS; POLYMERASE CHAIN REACTION; TIME FACTORS;

EID: 34047265819     PISSN: 02722712     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cll.2007.01.004     Document Type: Review

References (15)

1. Cantor C.R., and Smith C.L. Genomics: The Science and Technology Behind the Human Genome Project (1999), Wiley Interscience, New York, NY
2. Wilding P. Nucleic acid amplification in microchips. In: Cheng J., and Kricka L.J. (Eds). Biochip Technology (2003), Taylor & Francis Books, Inc, New York, NY 173-184
3. Kricka L.J., and Wilding P. Microchip PCR. Anal Bioanal Chem 377 (2003) 820-825
4. Lagally E.T., and Mathies R.A. Integrated genetic analysis microsystems. J Phys D Appl Phys 37 (2004) R245-R261
5. Roper M.G., Easley C.J., and Landers J.P. Advances in polymerase chain reaction on microfluidic chips. Anal Chem 77 (2005) 3887-3894
6. Spitzack K.D., and Ugaz V.M. PCR in miniaturized systems: big progress in little devices. In: Minteer S.D. (Ed). Microfluidic Techniques: Reviews and Protocols; Methods in Molecular Biology Series, Vol. 321 (2005), Humana Press, Totowa, NJ Chapter 10
7. Krishnan M., Ugaz V.M., and Burns M.A. PCR in a Rayleigh-Bénard convection cell. Science 298 (2002) 793
8. Braun D., Goddard N.L., and Libchaber A. Exponential DNA replication by laminar convection. Phys Rev Lett 91 (2003) 158103
9. Chen Z., Qian S., Abrams W.R., et al. Thermosiphon-based PCR reactor: experiment and modeling. Anal Chem 76 (2004) 3707-3715
10. Wheeler E.K., Benett W., Stratton P., et al. Convectively driven polymerase chain reaction thermal cycler. Anal Chem 76 (2004) 4011-4016
11. Krishnan M., Agrawal N., Burns M.A., et al. Reactions and fluidics in miniaturized natural convection systems. Anal Chem 76 (2004) 6254-6265
12. Chandrasekhar S. Hydrodynamic and Hydromagnetic Stability (1961), Oxford, Clarendon
13. Greif R. Natural circulation loops. J Heat Transf 110 (1988) 1243-1258
14. Innis M.A., Myambo K.B., Gelfand D.H., et al. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc Natl Acad Sci USA 85 (1988) 9436-9440
15. Agrawal N, Hassan YA, Ugaz VM. A pocket-sized convective PCR thermocycler. Angew Chem Int Edit 2007; in press.