Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide

BioTechniques

Volumn 41, Issue 4, 2006, Pages 469-473

  Rüttger, Anke ^a   Mollenhauer, Jürgen ^a,b   Löser, Reik ^c   Gütschow, Michael ^c   Wiederanders, Bernd ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b RUSH UNIVERSITY MEDICAL CENTER   (United States)

^c UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CATHEPSINS; MICROPLATE ASSAYS; MICROTITER PLATES; NITROSALICYLALDEHYDE;

ALDEHYDES; ANIMAL CELL CULTURE; CELL GROWTH; DERIVATIVES; ENZYME INHIBITION;

BIOASSAY;

2 NAPHTHYLAMINE; 4 METHOXY BETA NAPHTHYLAMINE; AMINOPEPTIDASE; BESTATIN; CATHEPSIN B; CATHEPSIN H; CATHEPSIN K; CATHEPSIN L; SALICYLALDEHYDE; UNCLASSIFIED DRUG; 5 NITROSALICYLALDEHYDE; 5-NITROSALICYLALDEHYDE; BENZALDEHYDE DERIVATIVE; CATHEPSIN; CYSTEINE PROTEINASE; DRUG DERIVATIVE; ENZYME INHIBITOR; LEUCINE;

ARTICLE; CELL ADHESION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME INHIBITOR INTERACTION; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PROTEIN DETERMINATION; ANIMAL; ARTICULAR CARTILAGE; C57BL MOUSE; CARTILAGE CELL; CATTLE; CELL LINE; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; METABOLISM; METHODOLOGY; MOUSE MUTANT; PERITONEUM MACROPHAGE; REPRODUCIBILITY;

MUS;

2-NAPHTHYLAMINE; AMINOPEPTIDASES; ANIMALS; BENZALDEHYDES; CARTILAGE, ARTICULAR; CATHEPSIN B; CATHEPSINS; CATTLE; CELL LINE; CHONDROCYTES; CYSTEINE ENDOPEPTIDASES; ENZYME INHIBITORS; HUMANS; LEUCINE; MACROPHAGES, PERITONEAL; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICROCHEMISTRY; REPRODUCIBILITY OF RESULTS; SUBSTRATE SPECIFICITY;

EID: 34047228186     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112259     Document Type: Article

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