Cloning, sequencing, expression, and antigenic characterization of rMSP4 from Anaplasma marginale isolated from Paraná State, Brazil

Genetics and Molecular Research

Volumn 6, Issue 1, 2007, Pages 15-22

  Kawasaki, P M ^a   Kano, F S ^a   Vidotto, O ^a   Vidotto, M C ^a  

^a STATE UNIVERSITY OF LONDRINA   (Brazil)

Author keywords

Anaplasma marginale; Bovine anaplasmosis; MSP4; Sequence of msp4

Indexed keywords

OUTER MEMBRANE PROTEIN; PROTEIN RMSP4; UNCLASSIFIED DRUG; BACTERIAL ANTIGEN; BACTERIAL DNA; BACTERIAL PROTEIN; BACTERIAL VACCINE; MEMBRANE PROTEIN; MSP4 PROTEIN, ANAPLASMA MARGINALE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANAPLASMA MARGINALE; ANAPLASMOSIS; ANTIGENIC MODULATION; ANTIGENICITY; ARTICLE; BRAZIL; ESCHERICHIA COLI; FUSION GENE; GENE EXPRESSION; GENE ISOLATION; GENE OVEREXPRESSION; GENE SEQUENCE; IMMUNE RESPONSE; IMMUNIZATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RABBIT; WESTERN BLOTTING; ANIMAL; CATTLE; CATTLE DISEASE; DNA SEQUENCE; GENETICS; IMMUNOBLOTTING; IMMUNOLOGY; ISOLATION AND PURIFICATION;

ANAPLASMA MARGINALE; BACTERIA (MICROORGANISMS); BOVINAE; ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

ANAPLASMA MARGINALE; ANAPLASMOSIS; ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BACTERIAL VACCINES; BRAZIL; CATTLE; CATTLE DISEASES; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOBLOTTING; MEMBRANE PROTEINS; POLYMERASE CHAIN REACTION; RABBITS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 34047214126     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Alleman AR and Barbet AF (1996). Evaluation of Anaplasma marginale major surface protein 3 (MSP3) as a diagnostic test antigen. J. Clin. Microbiol. 34: 270-276.
2. Alleman AR, Palmer GH, McGuire TC, McElwain TF, et al. (1997). Anaplasma marginale major surface protein 3 is encoded by a polymorphic, multigene family. Infect. Immun. 65: 156-163.
3. Altschul SF, Gish W, Miller W, Myers EW, et al. (1990). Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
4. Altschul SF, Madden TL, Schaffer AA, Zhang J, et al. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
5. Barbet AF, Lundgren A, Yi J, Rurangirwa FR, et al. (2000). Antigenic variation of Anaplasma marginale by expression of MSP2 mosaics. Infect. Immun. 68: 6133-6138.
6. Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
7. Brayton KA, Palmer GH, Lundgren A, Yi J, et al. (2002). Antigenic variation of Anaplasma marginale msp2 occurs by combinatorial gene conversion. Mol. Microbiol. 43: 1151-1159.
8. de la Fuente J, Passos LM, Van Den Bussche RA, Ribeiro MF, et al. (2004). Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil. Vet. Parasitol. 121: 307-316.
9. de la Fuente J, Torina A, Naranjo V, Caracappa S, et al. (2005). Genetic diversity of Anaplasma marginale strains from cattle farms in the province of Palermo, Sicily. J. Vet. Med. B Infect. Dis. Vet. Public Health 52: 226-229.
10. Dumler JS, Barbet AF, Bekker CP, Dasch GA, et al. (2001). Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int. J. Syst. Evol. Microbiol. 51: 2145-2165.
11. Kano FS, Vidotto O, Pacheco RC and Vidotto MC (2002). Antigenic characterization of Anaplasma marginale isolates from different regions of Brazil. Vet. Microbiol. 87: 131-138.
12. Oberle SM, Palmer GH and Barbet AF (1993). Expression and immune recognition of the conserved MSP4 outer membrane protein of Anaplasma marginale. Infect. Immun. 61: 5245-5251.
13. Oliveira JB, Madruga CR, Schenk MA, Kessler RH, et al. (2003). Antigenic characterization of four Brazilian isolates of Anaplasma marginale Rickettsiaceae: Ehrlichiae. Mem. Inst. Oswaldo Cruz 98: 395-400.
14. Palmer GH and McGuire TC (1984). Immune serum against Anaplasma marginale initial bodies neutralizes infectivity for cattle. J. Immunol. 133: 1010-1015.
15. Palmer GH and McElwain TF (1995). Molecular basis for vaccine development against anaplasmosis and babesiosis. Vet. Parasitol. 57: 233-253.
16. Palmer GH, Eid G, Barbet AF, McGuire TC, et al. (1994). The polymorphic multigene family. Infect. Immun. 62: 3808-3816.
17. Richey EJ (1981). Bovine anaplasmosis. In: Current veterinary therapy: food animal practice (Howard RJ, ed.). Saunders, Philadelphia, 767-772.
18. Sambrook J, Fritsch EF and Maniatis T (1989). Molecular cloning - A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
19. Tebele N, McGuire TC and Palmer GH (1991). Induction of protective immunity by using Anaplasma marginale initial body membranes. Infect. Immun. 59: 3199-3204.
20. Towbin H and Gordon J (1984). Immunoblotting and dot immunobinding- current status and outlook. J. Immunol. Methods 72: 313-340.
21. Visser ES, McGuire TC, Palmer GH, Davis WC, et al. (1992). The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species. Infect. Immun. 60: 5139-5144.