Purification and N-terminal sequencing of two presynaptic neurotoxic PLA2, neuwieditoxin-I and neuwieditoxin-II, from Bothrops neuwiedi pauloensis (Jararaca pintada) venom

Journal of Venomous Animals and Toxins Including Tropical Diseases

Volumn 13, Issue 1, 2007, Pages 103-121

  Borja Oliveira, C R ^a   Kassab, B H ^a   Soares, A M ^b   Toyama, M H ^a   Giglio, J R ^b   Marangoni, S ^a   Re, L ^c   Rodrigues Simioni, Léa ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

Author keywords

Bothrops neuwiedi pauloensis; Chick biventer cervicis; Loose patch clamp; Nerve muscle preparation; Neuromuscular junction; Neurotoxicity; Neuwieditoxin I; Neuwieditoxin II; PLA2 neurotoxin; Presynaptic action

Indexed keywords

BOTHROPS JARARACA; BOTHROPS NEUWIEDI PAULOENSIS;

EID: 34047213358     PISSN: 16789199     EISSN: 16789199     Source Type: Journal    
DOI: 10.1590/S1678-91992007000100008     Document Type: Article

References (53)

1. AIRD SD., KAISER II., LEWIS RV., KRUGGEL WG. A complete amino acid sequence for the basic subunit of crotoxin. Arch. Biochem. Biophys., 1986, 249, 296-300.
2. AIRD SD., KRUGGEL WG., KAISER II. Amino acid sequence of the basic subunit of Mojave toxin from the venom of the Mojave rattlesnake (Crotalus s. scutulatus). Toxicon, 1990, 28, 669-73.
3. 2 from Crotalus durissus cascavella rattlesnake (maracambóia) venom. J. Protein Chem., 2000, 19, 603-7.
4. BORJA-OLIVEIRA CR., DURIGON AM., VALLIN ACC., TOYAMA MH., SOUCCAR C., MARANGONI S., RODRIGUES-SIMIONI L. The pharmacological effects of Bothrops neuwiedi pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission. Braz. J. Med. Biol. Res., 2003, 36, 617-24.
5. BORJA-OLIVEIRA CR., SOARES AM., ZAMUNER SR., HYSLOP S., GIGLIO JR., PRADO-FRANCESCHI J., RODRIGUES-SIMIONI L. Intraspecific variation in the neurotoxic and myotoxic activities of Bothrops neuwiedi snake venoms. J. Venom. Anim. Toxins, 2002, 8, 88-101.
6. BUCARETCHI F., HERRERA SRF., HYSLOP S., BARACAT ECE., VIEIRA RJ. Snakebites by Bothrops spp in children in Campinas, São Paulo, Brazil. Rev. Inst. Med. Trop. São Paulo, 2001, 43, 329-33.
7. 2. Meth. Enzymol., 1991, 197, 75-9.
8. CINTRA AC., MARANGONI S., OLIVEIRA B., GIGLIO JR. Bothropstoxin-I: amino acid sequence and function. J. Protein Chem., 1993, 12, 57-64.
9. COGO JC., PRADO-FRANCESCHI J., CRUZ-HÖFLING MA., CORRADO AP., RODRIGUES-SIMIONI L. Effects of Bothrops insularis on the mouse and chick nerve-muscle preparation. Toxicon, 1993, 31, 1237-47.
10. 2 fraction. Toxicon, 1998, 36, 1323-32.
11. 2 homologue from the venom of Cerrophidion (Bothrops) godmani. Biochim. Biophys. Acta, 1998, 1384, 204-8.
12. DURIGON AM., BORJA-OLIVEIRA CR., DAL BELO CA., OSHIMA-FRANCO Y., COGO JC., LAPA AJ., SOUCCAR C., RODRIGUES-SIMIONI L. Neuromuscular activity of Bothrops neuwiedi pauloensis snake venom in mouse nerve-muscle preparations. J. Venom. Anim. Toxins incl. Trop. Dis., 2005, 11, 22-33.
13. FONTES MR., SOARES AM., RODRIGUES VM., FERNANDES AC., DA SILVA RJ, GIGLIO JR. Crystallization and preliminary X-ray diffraction analysis of a myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom. Biochim. Biophys. Acta, 1999, 1432, 393-5.
14. 2. Arch. Biochem. Biophys., 1991, 284, 352-9.
15. 2-homologue myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarara chica) from Argentina. Toxicon, 1999, 37, 1735-46.
16. GINSBORG BL., WARRINER J. The isolated chick biventer cervicis nerve-muscle preparation. Brit. J. Pharmacol., 1960, 15: 410-1.
17. HALPERT J., EAKER D. Amino acid sequence of a presynaptic neurotoxin from the venom of Notechis scutatus scutatus (Australian tiger snake). J. Biol. Chem., 1975, 250, 6990-7.
18. HARVEY AL., BARFARAZ A., THOMPSON E., FAIZ A., PRESTON S., HARRIS JB. Screening of snake venoms for neurotoxic and myotoxic effects using simple in vitro preparations from rodents and chicks. Toxicon, 1994, 32, 257-65.
19. HELUANY NF., HOMSI-BRANDEBURGO MI., GIGLIO JR., PRADO-FRANCESCHI J., RODRIGUES-SIMIONI L. Effects induced by bothropstoxin, a component from Bothrops jararacussu snake venom, on mouse and chick muscle preparations. Toxicon, 1992, 30, 1203-10.
20. 2. Toxicon, 1995, 35, 1149-55.
21. HOMSI-BRANDEBURGO MI., QUEIROZ LS., SANTO-NETO H., RODRIGUES-SIMIONI L., GIGLIO JR. Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin. Toxicon, 1988, 26, 615-27.
22. JOHNSON EK., OWNBY CL. Isolation of a myotoxin from the venom of Agkistrodon contortrix laticinctus (broad-banded copperhead) and pathogenesis of myonecrosis induced by it in mice. Toxicon, 1993, 31, 243-55.
23. KAISER II., GUTIERREZ JM., PLUMMER D., AIRD SD., ODELL GV. The amino acid sequence of a myotoxic phospholipase from the venom of Bothrops asper. Arch. Biochem. Biophys., 1990, 278, 319-25.
24. KATZ B., MILEDI R.. The binding of acetylcholine to receptors and its removal from the synaptic cleft. J. Physiol., 1973, 231, 549-74.
25. 2 enzymes. Structure, function and mechanism. New York: John Wiley & Sons Inc Chichester, 1997, 1-28.
26. KONDO K., NARITA K., LEE CY. Amino acid sequences of the two polypeptide chains in beta1-bungarotoxin from the venom of Bungarus multicinctus. J. Biochem., 1978, 83, 101-15.
27. 2-bungarotoxin from Bungarus multicinctus venom: the amino acid substitutions in the B chains. J. Biochem., 1982, 91, 1519-30.
28. KONDO K., ZHANG J., XU K., KAGAMIYAMA H. Amino acid sequence of a presynaptic neurotoxin, agkistrodotoxin, from the venom of Agkistrodon halys pallas. J. Biochem., 1989, 105, 196-203.
29. KORDAS M. On the role of junctional cholinesterase in determining the time course of the end-plate current. J. Physiol., 1977, 270, 133-50.
30. 2 from the horned puff adder (Bitis caudalis) venom. Toxicon, 1982, 20, 637-47.
31. LIND P., EAKER D. Amino-acid sequence of the alpha-subunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan (Oxyuranus s. scutellatus). European J. Biochem., 1982, 124, 441-7.
32. 2: quartenary structure and inhibition mechanism insights. Biochem. Biophys. Res. Commun., 2003, 311, 713-20.
33. 2 neurotoxins block nerve terminals? Trends Biochem. Sciences, 2000, 25, 266-70.
34. OSHIMA-FRANCO Y., HYSLOP S., CINTRA AC., GIGLIO JR., DA CRUZ-HOFLING MA., RODRIGUES-SIMIONI L. Neutralizing capacity of commercial bothropic antivenom against Bothrops jararacussu venom and bothropstoxin-I. Muscle Nerve, 2000, 23, 1832-9.
35. OSHIMA-FRANCO Y., LEITE GB., SILVA GH., CARDOSO DF., HYSLOP S., GIGLIO JR., DA CRUZ-HOFLING MA., RODRIGUES-SIMIONI L. Neutralization of the pharmacological effects of bothropstoxin-I from Bothrops jararacussu (jararacuçu) venom by crotoxin antiserum and heparin. Toxicon, 2001, 39, 1477-85.
36. 2 activity. J. Protein Chem., 1998, 17, 381-6.
37. RE L., BAROCCI S., CAPITANI C., VIVANI C., RICCI M., RINALDI L., PAOLUCCI G., SCARPANTONIO A., LEON-FERNANDEZ OS., MORALES MA. Effects of some natural extracts on the acetylcholine release at the mouse neuromuscular junction. Pharmacol. Res., 1999, 39, 239-45.
38. RE L., COLA V., FULGENZI G., MARINELLI F., CONCETTONI C., ROSSINI L. Postsynaptic effects of methoctramine at the mouse neuromuscular junction. Neuroscience, 1993, 57, 451-7.
39. RE L., GIUSTI P., CONCETTONI C., DI SARRA B. Computerized estimation of spontaneous and evoked acetylcholine release at the neuromuscular junction. J. Pharmacol. Meth., 1989, 22, 233-42.
40. RE L., MORETTI V., ROSSINI L., GIUSTI P. Sodium-activated potassium current in mouse diaphragm. FEBS Lett., 1990, 270, 195-7.
41. RIBEIRO LA., ALBUQUERQUE MJ., PIRES DE CAMPOS VAF., KATZ G., TAKAOKAM NY., LEBRÃO ML., JORGE MT. Óbitos por serpentes peçonhentas no Estado de São Paulo: avaliação de 43 casos, 1988/98. Rev. Assoc. Méd. Bras., 1998, 44, 312-8.
42. 2 from Vipera ammodytes ammodytes venom. Biochim. Biophys. Acta, 1985, 828, 306-12.
43. RODRIGUES-SIMIONI L., BORGESE N., CECCARELLI B. The effects of Bothrops jararacussu venom and its components on frog nerve-muscle preparation. Neuroscience, 1983, 10, 475-89.
44. RODRIGUES-SIMIONI L., ZAMUNER SR., COGO JC., BORJA-OLIVEIRA CR., PRADO-FRANCESCHI J., CRUZ-HOFLING MA., CORRADO AP. Pharmacological evidence for a presynaptic action of venoms from Bothrops insularis (jararaca ilhoa) and Bothrops neuwiedi (jararaca pintada). Toxicon, 2004, 43, 633-8.
45. SCHAGGER H., VON JAGOW G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa. Anal. Biochem., 1987, 166, 368-79.
46. 2 homologue from Bothrops neuwiedi venom. Arch. Biochem. Biophys., 2000, 378, 201-9.
47. STÜHMER W., ROBERTS WM., ALMERS W. The loose patch clamp. In: SAKMANN B AND NEHER E, Eds. Single Channel Recording. Plenum Press, New York; 1983, 123.
48. 2 homologue from Bothrops pirajai venom. Biochimie, 2000, 82, 245-50.
49. 2 from the venom of Taiwan habu (Trimeresurus mucrosquamatus). Biochem. J., 1995, 311, 895-900.
50. 2 from Agkistrodon piscivorus piscivorus venom. European J. Biochem., 1988, 176, 353-7.
51. 2. Biochim. Biophys. Acta, 1963, 70, 538-53.
52. 2 from the venom of the horned puff adder (Bitis caudalis). Toxicon, 1982, 20, 715-37.
53. ZAMUNER SR., PRADO-FRANCESCHI J., RODRIGUES-SIMIONI L. The screening of Bothrops venoms for neurotoxic activity using the chick biventer cervicis preparation. Toxicon, 1996, 34, 314-5.