Immobilization of Candida rugosa lipase on magnetized Dacron: Kinetic study

Artificial Cells, Blood Substitutes, and Biotechnology

Volumn 35, Issue 2, 2007, Pages 221-235

  Pimentel, M C B ^a   Leao A B F ^a   Melo, E H M ^a   Ledingham, W M ^a   Filho, J L Lima ^a   Sivewright, M ^b   Kennedy, J F ^c,d  

^a FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

^b UNIVERSITY OF ST ANDREWS   (United Kingdom)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^d Chembiotech Laboratories   (United Kingdom)

Author keywords

Candida rugosa lipase; Dacron; Magnetized support; Polyethylene terepthalate

Indexed keywords

CANDIDA RUGOSA LIPASE; DACRON; MAGNETIZED SUPPORT; SHORT CHAIN LENGTH;

FATTY ACIDS; FERROMAGNETIC MATERIALS; GLYCEROL; HYDROLYSIS; NITROGEN COMPOUNDS; POLYETHYLENE TEREPHTHALATES;

ENZYME IMMOBILIZATION;

DACRON; ESTER DERIVATIVE; FUNGAL ENZYME; GLYCEROL; HEXANE; LAURIC ACID DERIVATIVE; OLEIC ACID; PALMITIC ACID DERIVATIVE; SHORT CHAIN FATTY ACID; TRIACYLGLYCEROL LIPASE; TRIOLEIN;

ARTICLE; CANDIDA RUGOSA; CATALYSIS; COVALENT BOND; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME STABILITY; HYDROLYSIS; LIPOGENESIS; MICHAELIS CONSTANT; SOLUBILITY; STORAGE TEMPERATURE; THERMOSTABILITY;

2-PROPANOL; BIOTECHNOLOGY; CANDIDA; ENZYMES, IMMOBILIZED; ESTERS; HEAT; KINETICS; LIPASE; MAGNETICS; POLYETHYLENE TEREPHTHALATES; SOLVENTS; SUBSTRATE SPECIFICITY;

CANDIDA RUGOSA;

EID: 34047153398     PISSN: 10731199     EISSN: 15324184     Source Type: Journal    
DOI: 10.1080/10731190601188380     Document Type: Article

References (29)

1. Braun, B., Klein, E., López, J.L. (1995). Immobilization of Candida rugosa lipase to nylon fibers using its carbohydrate groups as the chemical link. Biotech. Bioeng. 51: 327-341.
2. Villeneuve, P. et al. (2000). Customizing lipases for biocatalysis : A survey of chemical, physical and molecular biological approaches. J. Mol. Catal. B: Enz. 9: 113-148.
3. Balcão, V.M., Paiva, A.L., Malcata, X. (1996). Bioreactors with immobilized lipases: State of the art. Enzyme Microb. Technol. 18: 392-416.
4. Sánchez, E.M. et al. (1996). Kinetic and enantioselective behavior of the lipase from Candida cylindracea: A comparative study between the soluble enzyme and the enzyme immobilized on the agarose and silica gels. Enzyme Microb. Technol. 18: 468-476.
5. Hung, T.C. et al. (2003). Binary immobilization of Candida rugosa lipase on chitosan. J. Mol. Catal. B: Enz. 26: 69-78.
6. Bayramoǧlu, G., Kaya, B., Arica, M.Y. (2005). Immobilization of Candida rugosa lipase onto spacer-arm attached poly (GMA-HEMA-EGDMA) microspheres. Food Chem. 92: 261-268.
7. Reetz, M.T. et al. (1998). Entrapment of lipases in hydrophobic magnetite-containing sol-gel materials: Magnetic separation of heterogeneous biocatalysts. J. Mol. Cat. A: Chem. 134: 251-258.
8. Guo, Z., Bai, S., Sun, Y. (2003). Preparation and characterization of immobilized lipase on magnetic hydrophobic microspheres. Enzyme Microb. Technol. 32: 776-782.
9. Bai, S. et al. (2005). Resolution of (±)-menthol by immobilized Candida rugosa lipase on superparamagnetic nanoparticles. Food Chem. (In press).
10. Liu, X. et al. (2005). Immobilization of lipase onto micron-size magnetic beads. J. Chrom. B. 822: 91-97.
11. Liu, X. et al. (2004). Synthesis of amino-silane modified superparamagnetic silica supports and their use for protein immobilization. Colloids Surf. A. 238: 127-131.
12. Carneiro-Leão, A.M.A., Oliveira, E.A., Carvalho-Jr, L.B. (1991). Immobilization of protein on ferromagnetic dacron. Appl. Biochem. Biotech. 31: 53-58.
13. Bruno, L.M. et al. (2005). Characterization of Mucor meihei lipase immobilized on polysiloxane-polyvinyl alcohol magnetic particles. World J. Microb. Biotechnol. 21: 189-192.
14. Winkler, U.K., Stuckmann, M. (1979). Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J. Bacteriol. 138: 663-670.
15. Pimentel, M.C.B. et al. (1994). Lipase from a Brazilian strain of Penicillium citrinum. Appl. Biochem. Biotech. 49: 59-73.
16. Pimentel, M.C.B. et al. (1997). Lipase from a Brazilian strain of Penicillium citrinum cultured in a simple and inexpensive medium: Heat-denaturation, kinetics and pH stability. Appl. Biochem. Biotechnol. 66: 185-195.
17. Ju, Y.H., Huang, F.C. (1995). Lipase immobilized on hydrophobic microporous polypropylene for the hydrolysis of palm kernel olein. Appl. Biochem. Biotechnol. 55: 17-26.
18. Kwon, D.Y., Rhee, J.S. (1986). A simple and rapid colorimetric method for determination of free fatty acids for lipase assay. JAOCS 63: 89-92.
19. Lowry, O.H. et al. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
20. Park, Y., Pastore, G. (1989). Esterificação de ácido graxo com glicerol por lipases microbianas, in Ciência e Tecnologia de Alimentos, Campinas University Publishers: Campinas, São Paulo, Brasil, 9: 163-171.
21. Nadruz, W. et al. (1994). Characterization of Candida rugosa lipase immobilised on alkylamine glass beads. Genetic Eng. Biotechnol. 14: 143-148.
22. Chiou, S.-H., Wu, W.-T. (2004). Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups. Biomaterials 25: 197-204.
23. Kilinç, A., Önal, S., Telefoncu, A. (2002). Chemical attachment of porcine pancreatic lipase to crosslinked poly(vinyl alcohol) by means of adipoyldichloride. Process Biochem. 38: 641-647.
24. Virto, M.D. et al. (1995). Kinetic properties of soluble and immobilized Candida rugosa lipase. Appl. Biochem. Biotechnol. 50: 127-136.
25. Gárcia-Alles, L.F., Gotor, V. (1998) Alcohol inhibition and specificity studies of lipase B from Candida antarctica in organic solvent. Biotechnol. Bioeng. 59: 163-170.
26. Knight, K. et al. (2000). Immobilization of lipase from Fusarium solani FS1. Braz. J. Microbiol. 31: 1-6.
27. Janssen, A.E.M., Vaidya, A.M., Halling, P.J. (1996). Substrate specificity and kinetics of Candida rugosa lipase in organic media. Enzyme Microb. Technol. 18: 340-346.
28. Zaidi, A. et al. (2002). Esterification of fatty acids using nylon-immobilized lipase in n-hexane: Kinetic parameters and chain-length effects. J. Biotechnol. 93: 209-216.
29. Wu, J.-C. et al. (2003). Hydrolytic reactions catalyzed by surfactant-coated Candida rugosa lipase in an organic-aqueous two phase system. Process Biochem. 39: 233-238.