Sesterterpene sulfates as isocitrate lyase inhibitors from tropical sponge Hippospongia sp.

Bioorganic and Medicinal Chemistry Letters

Volumn 17, Issue 9, 2007, Pages 2483-2486

  Lee, Hyi Seung ^a   Lee, Tae Hoon ^b   Yang, Seung Hwan ^a   Shin, Hee Jae ^a   Shin, Jongheon ^c   Oh, Ki Bong ^b,c  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

^b Seoul National University   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

Appressorium; Hippospongia sp.; ICL inhibition; Mgnaporthe grisea; Sponge

Indexed keywords

3 NITROPROPIONIC ACID; CARBON; ISOCITRATE LYASE; MALATE SYNTHASE; SESTERTERPENE; SULFATE;

ARTICLE; DRUG ISOLATION; DRUG POTENCY; FUNGUS; HYDROLYSIS; MGNAPORTHE GRISEA; NONHUMAN; RICE BLAST; SPONGE (PORIFERA);

ACETATES; ANIMALS; ANTIFUNGAL AGENTS; CHEMISTRY, PHARMACEUTICAL; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; GLUCOSE; INHIBITORY CONCENTRATION 50; ISOCITRATE LYASE; MODELS, CHEMICAL; PORIFERA; SULFATES; SULFURIC ACID ESTERS; TERPENES;

HIPPOSPONGIA; MAGNAPORTHE GRISEA;

EID: 33947726601     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.02.027     Document Type: Article

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18. 5 conidia/mL) with different concentrations of sesterterpene sulfates was placed on the hydrophobic side of GelBond and incubated in a moistened box at 24 °C for 14 h. For ICL inhibitor addition, we prepared concentrated stock solutions (10 mg/mL) in dimethylsulfoxide and added them to conidial suspension at appropriate dilutions (final solvent concentration <1%).
19. 2O 0.5 g/L, KCl 0.5 g/L, trace element 0.1%, and vitamin solution 0.1%) containing either sodium acetate or glucose as sole carbon source. Stock solutions of test compounds were prepared in dimethylsulfoxide and stored at -20 °C. Each stock solution was diluted with minimal growth medium to prepare serial dilutions in the range of 400-0.195 μg/ml prior to use. The minimum inhibitory concentration (MIC) was defined as the lowest concentration of compound at which no growth was observable after being incubated for 6 days at 28 °C.