Speeding up proton transfer in a fast enzyme: Kinetic and crystallographic studies on the effect of hydrophobic amino acid substitutions in the active site of human carbonic anhydrase II

Biochemistry

Volumn 46, Issue 12, 2007, Pages 3803-3813

  Fisher, S Zoë ^a   Tu, Chingkuang ^a   Bhatt, Deepa ^a   Govindasamy, Lakshmanan ^a   Agbandje McKenna, Mavis ^a   McKenna, Robert ^a,b   Silverman, David N ^a,b  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; CRYSTALLOGRAPHY; HYDRATION; PROTON TRANSFER; SUBSTITUTION REACTIONS;

BULK SOLUTION; CARBONIC ANHYDRASE II;

ENZYME KINETICS;

AMINO ACID; CARBON DIOXIDE; CARBONATE DEHYDRATASE II; ESTERASE; HISTIDINE; HYDROXIDE; OXYGEN 18; PROTON; WATER; ZINC;

AMINO ACID SUBSTITUTION; ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME KINETICS; ESCHERICHIA COLI; HUMAN; HYDROGEN BOND; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS; SPECTROPHOTOMETRY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; BINDING SITES; CARBON DIOXIDE; CARBONIC ANHYDRASE II; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; HISTIDINE; HUMANS; HYDROGEN BONDING; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTONS; WATER;

EID: 33947684326     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi602620k     Document Type: Article

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