The sulfhydryl group of Cys138 of rusticyanin from Acidithiobacillus ferrooxidans is crucial for copper binding

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1774, Issue 4, 2007, Pages 519-525

  Zeng, Jia ^a   Geng, Meimei ^a   Liu, Yuandong ^a   Xia, Lexian ^a   Liu, Jianshe ^a   Qiu, Guanzhou ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Acidithiobacillus ferrooxidans; Cysteine; Expression; His tag; Purification; Rusticyanin; Sulfhydryl group

Indexed keywords

CYSTEINE; MUTANT PROTEIN; RUSTICYANIN; THIOL GROUP;

ACIDITHIOBACILLUS FERROOXIDANS; AFFINITY CHROMATOGRAPHY; ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; ESCHERICHIA COLI; GENE OVEREXPRESSION; METAL BINDING; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

ACIDITHIOBACILLUS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AZURIN; CLONING, MOLECULAR; COPPER; CYSTEINE; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SPECTROPHOTOMETRY, ATOMIC;

ACIDITHIOBACILLUS FERROOXIDANS; ESCHERICHIA COLI;

EID: 33947647415     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2007.02.008     Document Type: Article

References (24)

1. Ingledew W.J. Thiobacillus ferrooxidans. The bioenergetics of an acidophilic chemoautotroph. Biochim. Biophys. Acta 683 (1982) 89-117
2. Rawlings D.E. The molecular genetics of Thiobacillus ferrooxidans and other mesophilic, acidophilic, chemolithotrophic, iron- or sulfur-oxidizing bacteria. Hydrometallurgy 59 (2001) 187-201
3. Yamanaka T., and Fukumori Y. Molecular aspects of the electron transfer system which participate in the oxidation of ferrous ion by Thiobacillus ferrooxidans. FEMS Microbiol. Rev. 17 (1995) 401-413
4. Ronk M., and Shively J.E. Amino acid sequence of the blue copper protein rusticyanin from Thiobacillus ferrooxidans. Biochemistry 30 (1991) 9435-9442
5. Appia-Ayme C., Guiliani N., Ratouchniak J., and Bonnefoy V. Characterization of an operon encoding two c-type cytochromes, an aa(3)-type cytochrome oxidase, and rusticyanin in Thiobacillus ferrooxidans ATCC 33020. Appl. Environ. Microbiol. 65 (1999) 4781-4787
6. Yarzabal A., Appia-Ayme C., Ratouchniak J., and Bonnefoy V. Regulation of the expression of the Acidithiobacillus ferrooxidans rus operon encoding two cytochromes c, a cytochrome oxidase and rusticyanin. Microbiology 150 (2004) 2113-2123
7. Rohwerder T., Gehrke T., Kinzler K., and Sand W. Bioleaching review part A: progress in bioleaching: fundamentals and mechanisms of bacterial metal sulfide oxidation. Appl. Microbiol. Biotechnol. 63 (2003) 239-248
8. Rawlings D.E. Characterization and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates. Microbial. Cell Factories 4 (2005) 13
9. Ingledew W.J., and Cobley J.G. A potentiometric and kinetic study on the respiratory chain of ferrous iron grown Thiobacillus ferrooxidans. Biochim. Biophys. Acta 590 (1980) 141-148
10. Walter R.L., Ealick S.E., Friedman A.M., Blake R.C., Proctor P., and Shoham M. Multiple wavelength anomalous diffraction (MAD) crystal structure of rusticyanin: a highly oxidizing cupredoxin with extreme acid stability. J. Mol. Biol. 263 (1996) 730-751
11. Guidici-Orticoni M.T., Guerlesquin F., Bruschi M., and Nitschke W. Interaction-induced redox switch in the electron-transfer complex rusticyanin-cytochrome c(4). J. Biol. Chem. 274 (1999) 30365-30369
12. Hall J.F., Kanbi L.D., Harvey I., Murphy L.M., and Hasnain S.S. Modulating the redox potential and acid stability of rusticyanin by site-directed mutagenesis of Ser86. Biochemistry 37 (1998) 11451-11458
13. Kanbi L.D., Antonyuk S., Hough M.A., Hall J.F., Dodd F.E., and Hasnain S.S. Crystal structures of the Met148Leu and Ser86Asp mutants of rusticyanin from Thiobacillus ferrooxidans: insights into the structural relationship with the cupredoxins and the multi copper proteins. J. Mol. Biol. 320 (2002) 263-275
14. Casimiro D.R., Toy-Palmer A., Blake R.C., and Dyson H.J. Gene synthesis, high-level expression, and mutagenesis of Thiobacillus ferrooxidans rusticyanin: His 85 is a ligand to the blue copper center. Biochemistry 34 (1995) 6640-6648
15. Barrett M.L., Harvey I., Sundararajan M., Surendran R., Hall J.F., Ellis M.J., Hough M.A., Strange R.W., Hillier I.H., and Hasnain S.S. Atomic resolution crystal structures, EXAFS, and quantum chemical studies of rusticyanin and its two mutants provide insight into its unusual properties. Biochemistry 45 (2006) 2927-2939
16. Hall J.F., Kanbi L.D., Strange R.W., and Hasnain S.S. Role of the axial ligand in type 1 Cu centers studied by point mutations of Met148 in rusticyanin. Biochemistry 38 (1999) 12675-12680
17. Sodeoka M., Larson C., Chen L., Land W., and Verdine G. A multifunctional plasmid for protein expression by ECPCR: overproduction of the p50 subunit of NF-KB. Bioorg. Med. Chem. Lett. 3 (1993) 1095-1100
18. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
19. Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
20. Bengrine A., Guiliani N., Appia-Ayme C., Jedlicki E., Holmes D.S., Chippaux M., and Bonnefoy V. Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC 33020 strain. Biochim. Biophys. Acta 1443 (1998) 99-112
21. Bruscella P., Cassagnaud L., Ratouchniak J., Brasseur G., Lojou E., Amils R., and Bonnefoy V. The HiPIP from the acidophilic Acidithiobacillus ferrooxidans is correctly processed and translocated in Escherichia coli, in spite of the periplasm pH difference between these two micro-organisms. Microbiology 151 (2005) 1421-1431
22. Palmer T., and Berks B.C. Moving folded proteins across the bacterial cell membrane. Microbiology 149 (2003) 547-556
23. Cox J.C. EPR studies of the blue copper protein, rusticyanin. FEBS Lett. 93 (1978) 157-160
24. Thompson J., Gibson T., Plewniak F., Jeanmougin F., and Higgins D. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24 (1997) 4876-4882