Development and application of a novel multiplex polymerase chain reaction for semi-quantitation of Human Cytomegalovirus in clinical specimens

Journal of Virological Methods

Volumn 141, Issue 2, 2007, Pages 166-172

  Madhavan, H N ^a   Sowmya, P ^a   Therese, K L ^a   Malathi, J ^a  

^a VISION RESEARCH FOUNDATION   (India)

Author keywords

Human Cytomegalovirus (HCMV); Multiplex PCR; pp65 Antigenemia assay; Real time PCR

Indexed keywords

GLYCOPROTEIN O; UNCLASSIFIED DRUG; VIRUS GLYCOPROTEIN;

ARTICLE; CONTROLLED STUDY; EVALUATION; GO GENE; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; IMMUNE DEFICIENCY; KIDNEY TRANSPLANTATION; MAJOR CLINICAL STUDY; MORBIDITY; MORTALITY; MTR II GENE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RECIPIENT; STANDARDIZATION; UL 83 GENE; VIRUS GENE; VIRUS GENOME; VIRUS STRAIN;

CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA PRIMERS; HUMANS; MEMBRANE GLYCOPROTEINS; PHOSPHOPROTEINS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRAL ENVELOPE PROTEINS; VIRAL LOAD; VIRAL MATRIX PROTEINS;

HUMAN HERPESVIRUS 5;

EID: 33947607794     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.12.003     Document Type: Article

References (23)

1. Allice T., Enrietto M., Pittaluga F., Varetto S., Alessandro F., Giovanna M., and Ghisetti V. Quantitation of Cytomegalovirus DNA by real-time polymerase chain reaction in peripheral blood specimens of patients with solid organ transplants: comparison with end-point PCR and pp65 antigen test. J. Med. Virol. 78 (2006) 915-922
2. Boeckh M., and Boivin G. Quantitation of Cytomegalovirus: methodologic aspects and clinical applications. Clin. Microbiol. Rev. 11 (1998) 533-554
3. Caballero O.L., Menezes C.L., Costa M.C., Fernandes S.C., Anacleto T.M., de Oliveira R.M., Viotti E.A., Tavora E.R., Vilaca S.S., Sabbaga E., de Paula F.J., Tavora P.F., Villa L.L., and Simpson A.J. Highly sensitive single-step PCR protocol for diagnosis and monitoring of Human Cytomegalovirus infection in renal transplant recipients. J. Clin. Microbiol. 35 (1997) 3192-3197
4. Gault E., Michel Y., Dehee A., Belabani C., Nicolas J.C., and Chenon A.G. Quantification of Human Cytomegalovirus DNA by real-time PCR. J. Clin. Microbiol. 39 (2001) 772-775
5. Gouarin S., Gault E., Vabret A., Cointe D., Rozenberg F., Keros G.L., Barjot P., Chenon G.A., Lebon P., and Freymuth F. Real-time PCR quantification of human cytomegalovirus DNA in amniotic fluid samples from mothers with primary infection. J. Clin. Microbiol. 40 (2002) 1767-1772
6. Ho S.K.N., Lo C.Y., Cheng I.K.P., and Chan T.M. Rapid Cytomegalovirus pp65 antigenemia assay by direct erythrocyte lysis and immunofluorescence staining. J. Clin. Microbiol. 36 (1998) 638-640
7. Ikewaki J., Ohtsuka E., Kawano R., Ogata M., Kikuchi H., and Nasu M. Real-time PCR assay compared to nested PCR and antigenemia assays for detecting Cytomegalovirus reactivation in adult T-cell leukemia-lymphoma patients. J. Clin. Microbiol. 41 (2003) 4382-4387
8. Kalpoc J.S., Krocs A.C.M., Jong M.D., Schinkel J., Brouwer C.S., Beersma M.F.C., and Claas E.C.J. Validation of clinical application of Cytomegalovirus plasma DNA load measurement and definition of treatment criteria by analysis of correlation to antigen detection. J. Clin. Microbiol. 42 (2004) 1498-1504
9. Landry M.L., Ferguson D., Cohen S., Huber K., and Wetherill P. Effect of delayed specimen processing on cytomegalovirus antigenemia test results. J. Clin. Microbiol. 33 (1995) 257-259
10. Madhavan H.N., and Priya K. Use of nested polymerase chain reaction (nPCR) for the detection of Cytomegalovirus (CMV) in clinical specimens. Indian J. Med. Res. 115 (2002) 5-10
11. Mazulli T., Drew L.W., Lieberman B.Y., McMullen D.J., Kohn D.J., Isada C., Moussa G., Chua R., and Walmsley S. Multicenter comparison of the digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of Cytomegalovirus viremia. J. Clin. Microbiol. 37 (1999) 958-963
12. Nitsche A., Steuer N., Schmidt C.A., Landt O., and Siegert W. Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA. Clin. Chem. 45 (1999) 1932-1937
13. Pang X.L., Chui L., Fenton J., LeBlanc B., and Preiksaitis J.K. Comparison of LightCycler-based PCR, COBAS amplicor CMV monitor and pp65 antigenemia assays for quantitative measurement of Cytomegalovirus viral load in peripheral blood specimens from patients after solid-organ transplantation. J. Clin. Microbiol. 41 (2003) 3167-3174
14. Piiparinen H., Hockerstedt K., Lappalainen M., Suni J., and Lautenschlager I. Monitoring of viral load by quantitative plasma PCR during active cytomegalovirus infection of individual liver transplant patients. J. Clin. Microbiol. 40 (2002) 2945-2952
15. Piiparinen H., Hockerstedt K., Gronhagen-Riska C., and Lautenschlager I. Comparison of two quantitative CMV PCR tests, Cobas Amplicor CMV monitor and Taqman assay and pp65 antigenemia assay in the determination of viral loads from peripheral blood of organ transplant patients. J. Clin. Virol. 30 (2004) 258-266
16. Rasmussen L., Geissler A., Cowan C., Chase A., and Winters M. The genes encoding the gCIII complex of Human Cytomegalovirus exist in highly diverse combinations in clinical isolates. J. Virol. 76 (2002) 10841-10848
17. Razonable R.R., Paya C.V., and Smith T.F. Role of the laboratory in diagnosis and management of cytomegalovirus infection in hematopoietic stem cell and solid-organ transplant recipients. J. Clin. Microbiol. 40 (2002) 746-752
18. Roberts T.C., Brennan D.C., Buller R.S., Keener G.M., Schnitzler M.A., Sternhell K.E., Garlock K.A., Singer G.G., and Storch G.A. Quantitative polymerase chain reaction to predict occurrence of symptomatic Cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients. J. Infect. Dis. 178 (1998) 626-635
19. Sowmya P., Madhavan H.N., and Therese K.L. Evaluation of three polymerase chain reaction tests targeting morphological transforming region II, UL-83 gene and glycoprotein O gene for the detection of Human Cytomegalovirus genome in clinical specimens of immunocompromised patients in Chennai, India. Virol. J. 3 (2006) 20
20. Seropian S., Ferguson D., Salloum E., Cooper D., and Landry M.L. Lack of reactivity to CMV pp65 antigenemia testing in a patient with CMV disease following allogeneic bone marrow transplant. Bone Marrow Transplant. 22 (1998) 507-509
21. Shapiro A.M., Bain V.G., Preiksaitis J.K., Ma M.M., Issa S., and Kneteman N.M. Ogilvie's syndrome associated with acute cytomegaloviral infection after liver transplantation. Transplant. Int. 13 (2000) 41-45
22. Weber B., Nestler U., Ernst W., Rabenau H., Braner J., Birkenbach A., Scheuermann E.H., Schoeppe W., and Doerr H.W. Low correlation of human cytomegalovirus DNA amplification by polymerase chain reaction with cytomegalovirus disease in organ transplant recipients. J. Med. Virol. 43 (1994) 187-193
23. Yun Z., Fuchs I.L., Ljungman P., and Vahlne A. Real-time monitoring of cytomegalovirus infections after stem cell transplantation using the TaqMan polymerase chain reaction assays. Transplantation 69 (2000) 1733-1736