Highly sensitive single-step PCR protocol for diagnosis and monitoring of human cytomegalovirus infection in renal transplant recipients

Journal of Clinical Microbiology

Volumn 35, Issue 12, 1997, Pages 3192-3197

  Caballero, Otávia L ^a,b,c   Menezes, Carla L P ^d   Costa, Maria Clara S L ^e   Fernandes, Silvana C ^d   Anacleto, Taís M ^a   De Oliveira, Ricardo Manoel ^d   Viotti, Estevam A ^f   Távora, Eduardo R F ^f   Vilaça, Sandra S ^f   Sabbaga, Emil ^c   De Paula, Flávio J ^c   Távora, Paula F ^g   Villa, Luisa L ^a   Simpson, Andrew J G ^a  

^a LUDWIG INSTITUTE FOR CANCER RESEARCH   (Brazil)

^b FEDERAL UNIVERSITY OF TRIÂNGULO MINEIRO   (Brazil)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

^d A C CAMARGO CANCER CENTER   (Brazil)

^e Hemagen S/A   (Brazil)

^f HOSPITAL FELÍCIO ROCHO   (Brazil)

^g Laboratório São Marcos   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

POLYACRYLAMIDE GEL; VIRUS DNA;

ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS INFECTION; HUMAN; HUMAN CELL; KIDNEY TRANSPLANTATION; LEUKOCYTE; MAJOR CLINICAL STUDY; PATIENT MONITORING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SILVER STAINING; VIRUS GENOME;

CYTOMEGALOVIRUS; DNA VIRUSES; HUMAN HERPESVIRUS 5;

EID: 0030684794     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.12.3192-3197.1997     Document Type: Article

References (22)

1. Albert, J., and E. M. Fenyö. 1990. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers. J. Clin. Microbiol. 28:1560-1564.
2. Allen, R. D., P. E. Pellett, J. A. Stewart, and M. Koopmans. 1995. Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA. J. Clin. Microbiol. 33:725-728.
3. Borg, K. L., S. A. Nordbø, P. Winge, and A. Dalen. 1995. Detection of cytomegalovirus using 'boosted' nested PCR. Mol. Cell. Probes 9:251-257.
4. Chou, Q., M. Russel, D. E. Birch, J. Raymond, and W. Bloch. 1992. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20:1717-1723.
5. Gerdes, J. C., E. K. Spees, K. Fitting, J. Hiraki, M. Sheehan, D. Duda, T. Jarvi, C. Roehl, and A. D. Robertson. 1993. Prospective study utilizing a quantitative polymerase chain reaction for detection of cytomegalovirus DNA in the blood of renal transplant patients. Transplant. Proc. 25:1411-1413.
6. Kühn, J. E., T. Wendland, P. Schäfer, K. Möhring, U. Wieland, M. Elgas, and H. J. Eggers. 1994. Monitoring of renal allograft recipients by quantitation of human cytomegalovirus genomes in peripheral blood leukocytes. J. Med. Virol. 44:398-405.
7. Kulski, J. K. 1994. Quantitation of human cytomegalovirus DNA in leukocytes by end-point titration and duplex polymerase chain reaction. J. Virol. Methods 49:195-208.
8. Miller, M. J., S. Bovey, K. Pado, D. A. Bruckner, and E. A. Wagar. 1994. Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay. J. Clin. Microbiol. 32:5-10.
9. Persing, D. H., T. F. Smith, F. C. Tenover, and T. J. White (ed.). 1993. Diagnostic molecular microbiology: principles and applications. American Society for Microbiology, Washington, D.C.
10. Raeymaekers, L. 1995. A commentary on the practical applications of competitive PCR. Genome Res. 5:91-94.
11. Rasmussen, L., S. Morris, D. Zipeto, J. Feseel, R. Wolitz, A. Dowling, and T. C. Merigan. 1995. Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis. J. Infect. Dis. 171:177-182.
12. Rüger, B., S. Klages, B. Walla, J. Albrecht, B. Fleckenstein, P. Tomlinson, and B. Barrel. 1987. Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus. J. Virol. 61:446-453.
13. Saiki, R. K., D. H. Gelfand, S. Stoffel, S. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis, and H. A. Erlich. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491.
14. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
15. Sanguinetti, C. J., E. Dias Neto, and A. J. G. Simpson. 1994. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. BioTechniques 17:915-919.
16. Shibata, D., W. J. Martin, M. D. Appleman, D. M. Causey, J. M. Leedom, and N. Arnheim. 1988. Detection of cytomegalovirus DNA in peripheral blood of patients infected with human immunodeficiency virus. J. Infect. Dis. 158:1185-1192.
17. Shinkai, M., S. A. Bozzette, W. Powderly, P. Frame, and S. A. Spector. 1997. Utility of urine and leukocyte cultures and plasma DNA polymerase chain reaction for identification of AIDS patients at risk of developing human cytomegalovirus disease. J. Infect. Dis. 175:302-308.
18. Stenberg, R. M., D. R. Thomsen, and M. F. Stinski. 1984. Structural analysis of the major intermediate early gene of human cytomegalovirus. J. Virol. 49:190-199.
19. Sykes, P. J., S. H. Neoh, M. J. Brisco, E. Hughes, J. Condon, and A. A. Morley. 1992. Quantitation of targets for PCR by use of limiting dilution. BioTechniques 13:444-449.
20. Zhang, X.-Y., and M. Erlich. 1994. Detection and quantitation of low numbers of chromosomes containing bcl-2 oncogene translocations using semi-nested PCR. BioTechniques 16:502-507.
21. Zimmermann, K., K. Pischinger, and J. W. Mannhalter. 1994. Nested primer PCR detection limits of HIV-1 in the background of increasing numbers of lysed cells. BioTechniques 17:18-20.
22. Zimmerman, K., and J. W. Mannhalter. 1996. Technical aspects of quantitative competitive PCR. BioTechniques 21:268-279.