The SUF iron-sulfur cluster biosynthetic machinery: Sulfur transfer from the SUFS-SUFE complex to SUFA

FEBS Letters

Volumn 581, Issue 7, 2007, Pages 1362-1368

  Sendra, Maïté ^a   Ollagnier de Choudens, Sandrine ^a   Lascoux, David ^b   Sanakis, Yiannis ^c   Fontecave, Marc ^a  

^a Institut de Biosciences et Biotechnologies de Grenoble BIG   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c INSTITUTE OF MATERIALS SCIENCE   (Greece)

Author keywords

Biosynthesis; Iron sulfur cluster; Mass spectrometry; M ssbauer spectroscopy; Site directed mutagenesis

Indexed keywords

APOPROTEIN; BACTERIAL PROTEIN; CYSTATHIONINE GAMMA LYASE; IRON; MUTANT PROTEIN; PROTEIN SUFA; PROTEIN SUFE; PROTEIN SUFS; SULFUR; UNCLASSIFIED DRUG;

ARTICLE; BINDING COMPETITION; BIOSYNTHESIS; COVALENT BOND; ESCHERICHIA COLI; MASS SPECTROMETRY; MOSSBAUER SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 33947540909     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.02.058     Document Type: Article

References (34)

1. Johnson M.K. Iron-sulfur proteins: new roles for old clusters. Curr. Opin. Chem. Biol. 2 (1998) 173-181
2. Fontecave M., Choudens S.O., Py B., and Barras F. Mechanisms of iron-sulfur cluster assembly: the SUF machinery. J. Biol. Inorg. Chem. (2005) 1-9
3. Zheng L., Cash V.L., Flint D.H., and Dean D.R. Assembly of iron-sulfur clusters. Identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii. J. Biol. Chem. 273 (1998) 13264-13272
4. Takahashi Y., and Nakamura M. Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J. Biochem. (Tokyo) 126 (1999) 917-926
5. Lill R., and Kispal G. Maturation of cellular Fe-S proteins: an essential function of mitochondria. Trends Biochem. Sci. 25 (2000) 352-356
6. Tong W.H., and Rouault T. Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells. EMBO J. 19 (2000) 5692-5700
7. Nachin L., Loiseau L., Expert D., and Barras F. SufC: an unorthodox cytoplasmic ABC/ATPase required for [Fe-S] biogenesis under oxidative stress. EMBO J. 22 (2003) 427-437
8. Outten F.W., Djaman O., and Storz G. A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli. Mol. Microbiol. 52 (2004) 861-872
9. Mihara H., and Esaki N. Bacterial cysteine desulfurases: their function and mechanisms. Appl. Microbiol. Biotechnol. 60 (2002) 12-23
10. Loiseau L., Ollagnier-de-Choudens S., Nachin L., Fontecave M., and Barras F. Biogenesis of Fe-S cluster by the bacterial Suf system: SufS and SufE form a new type of cysteine desulfurase. J. Biol. Chem. 278 (2003) 38352-38359
11. Ollagnier-de-Choudens S., Lascoux D., Loiseau L., Barras F., Forest E., and Fontecave M. Mechanistic studies of the SufS-SufE cysteine desulfurase: evidence for sulfur transfer from SufS to SufE. FEBS Lett. 555 (2003) 263-267
12. Ollagnier-de-Choudens S., Mattioli T., Takahashi Y., and Fontecave M. Iron-sulfur cluster assembly: characterization of IscA and evidence for a specific and functional complex with ferredoxin. J. Biol. Chem. 276 (2001) 22604-22607
13. Ollagnier-de Choudens S., Nachin L., Sanakis Y., Loiseau L., Barras F., and Fontecave M. SufA from Erwinia chrysanthemi. Characterization of a scaffold protein required for iron-sulfur cluster assembly. J. Biol. Chem. 278 (2003) 17993-18001
14. Krebs C., Agar J.N., Smith A.D., Frazzon J., Dean D.R., Huynh B.H., and Johnson M.K. IscA, an alternate scaffold for Fe-S cluster biosynthesis. Biochemistry 40 (2001) 14069-14080
15. Agar J.N., Krebs C., Frazzon J., Huynh B.H., Dean D.R., and Johnson M.K. IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU. Biochemistry 39 (2000) 7856-7862
16. Ding H., and Clark R.J. Characterization of iron binding in IscA, an ancient iron-sulphur cluster assembly protein. Biochem. J. 379 (2004) 433-440
17. Morimoto K., Yamashita E., Kondou Y., Lee S.J., Arisaka F., Tsukihara T., and Nakai M. The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold. J. Mol. Biol. 360 (2006) 117-132
18. Outten F.W., Wood M.J., Munoz F.M., and Storz G. The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli. J. Biol. Chem. 278 (2003) 45713-45719
19. Silberg J.J., Tapley T.L., Hoff K.G., and Vickery L.E. Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU. J. Biol. Chem. 279 (2004) 53924-53931
20. Ollagnier-de-Choudens S., Sanakis Y., and Fontecave M. SufA/IscA: reactivity studies of a class of scaffold proteins involved in [Fe-S] cluster assembly. J. Biol. Inorg. Chem. 9 (2004) 828-838
21. Wollenberg M., Berndt C., Bill E., Schwenn J.D., and Seidler A. A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins. Eur. J. Biochem. 270 (2003) 1662-1671
22. Fontecave M., Choudens S.O., Py B., and Barras F. Mechanisms of iron-sulfur cluster assembly: the SUF machinery. J. Biol. Inorg. Chem. 10 (2005) 713-721
23. Johnson D.C., Dean D.R., Smith A.D., and Johnson M.K. Structure, function, and formation of biological iron-sulfur clusters. Annu. Rev. Biochem. 74 (2005) 247-281
24. Fish W.W. Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples. Meth. Enzymol. 158 (1988) 357-364
25. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
26. Beinert H. Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins. Anal. Biochem. 131 (1983) 373-378
27. Field T.M., McCourt J.L., and McBryde W.A.E. Composition and stability of iron and copper citrate complexes in aqueous solution. Can. J. Chem. 52 (1974) 3119-3124
28. Wright C.M., Christman G.D., Snellinger A.M., Johnston M.V., and Mueller E.G. Direct evidence for enzyme persulfide and disulfide intermediates during 4-thiouridine biosynthesis. Chem. Commun. (Camb.) (2006) 3104-3106
29. Smith A.D., Frazzon J., Dean D.R., and Johnson M.K. Role of conserved cysteines in mediating sulfur transfer from IscS to IscU. FEBS Lett. 579 (2005) 5236-5240
30. Wada K., Hasegawa Y., Gong Z., Minami Y., Fukuyama K., and Takahashi Y. Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer. FEBS Lett. 579 (2005) 6543-6548
31. Loiseau L., Ollagnier-de Choudens S., Lascoux D., Forest E., Fontecave M., and Barras F. Analysis of the heteromeric CsdA-CsdE cysteine desulfurase, assisting Fe-S cluster biogenesis in Escherichia coli. J. Biol. Chem. 280 (2005) 26760-26769
32. Ding H., Clark R.J., and Ding B. IscA mediates iron delivery for assembly of iron-sulfur clusters in IscU under the limited accessible free iron conditions. J. Biol. Chem. 279 (2004) 37499-37504
33. Ding H., Harrison K., and Lu J. Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU. J. Biol. Chem. 280 (2005) 30432-30437
34. Layer G., Ollagnier-de Choudens S., Sanakis Y., and Fontecave M. Iron-sulfur cluster biosynthesis: characterization of Escherichia coli CYaY as an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU. J. Biol. Chem. 281 (2006) 16256-16263