Analysis of aqueous glue coating proteins on the silk fibers of the cob weaver, Latrodectus hesperus

Biochemistry

Volumn 46, Issue 11, 2007, Pages 3294-3303

  Hu, Xiaoyi ^a,c   Yuan, Jing ^a   Wang, Xiaodong ^a   Vasanthavada, Keshav ^a   Falick, Arnold M ^b   Jones, Patrick R ^a   La Mattina, Coby ^a   Vierra, Craig A ^a  

^a UNIVERSITY OF THE PACIFIC   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELUCIDATION; LOW-MOLECULAR MASS (LMM); PHOSPHORYLATED GLYCOPROTEINS; SPIDER GLUE;

LIPIDS; MASS SPECTROMETRY; SILK; SOLUTIONS; WEAVING;

PROTEINS;

AMINO ACID; COMPLEMENTARY DNA; GLUE PROTEIN; GLYCOPROTEIN; INORGANIC COMPOUND; LIPID; MESSENGER RNA; METAL; ORGANIC COMPOUND; PEPTIDE; SILK FIBROIN; SPIDER COATING PEPTIDE 1; SPIDER COATING PEPTIDE 2; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AQUEOUS SOLUTION; ARTICLE; BINDING AFFINITY; CHEMICAL COMPOSITION; DNA ISOLATION; LATRODECTUS HESPERUS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PREDATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEOMICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SILK; SPIDER; TANDEM MASS SPECTROMETRY;

ADHESIVES; AMINO ACID SEQUENCE; ANIMALS; BLACK WIDOW SPIDER; FIBROINS; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; OVUM; SEQUENCE ALIGNMENT; SILK; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

LIPIDS; MASS SPECTROMETERS; PROTEINS; SILK; WEAVING;

ARANEAE; LATRODECTUS; LATRODECTUS HESPERUS;

EID: 33947416583     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi602507e     Document Type: Article

References (33)

1. Gosline, J. M., DeMont, M. E., Denny, M. W. (1986) The structure and properties of spider silk, Endeavour 10, 31-43.
2. Vollrath, F. (1992) Spider webs and silk, Sci. Am. 266, 70-76.
3. Guerette, P. A., Ginzinger, D. G., Weber, B. H., and Gosline, J. M. (1996) Silk properties determined by gland-specific expression of a spider fibroin gene family, Science 272, 112-115.
4. Gatesy, J., Hayashi, C., Motriuk, D., Woods, J., and Lewis, R. (2001) Extreme diversity, conservation, and convergence of spider silk fibroin sequences, Science 291, 2603-2605.
5. Hayashi, C., and Lewis, R. V. (1998) Evidence from flagelliform silk cDNA for the structural basis of elasticity and modular nature of spider silks, J. Mol. Biol. 275, 773-784.
6. Hu, X., Lawrence, B., Kohler, K., Falick, A. M., Moore, A. M., McMullen, E., Jones, P. R., and Vierra, C. (2005) Araneoid egg case silk: A fibroin with novel ensemble repeat units from the black widow spider Latrodectus hesperus, Biochemistry 44, 10020-10027.
7. Tian, M., and Lewis, R. V. (2005) Molecular characterization and evolutionary study of spider tubuliform (eggcase) silk protein, Biochemistry 44, 8006-8012.
8. Hayashi, C. Y., Blackledge, T. A., and Lewis, R. V. (2004) Molecular and mechanical characterization of aciniform silk: Uniformity of iterated sequence modules in a novel member of the spider silk fibroin gene family, Mol. Biol. Evol. 21, 1950-1959.
9. Beckwitt, R., and Arcidiacono, S. (1994) Sequence conservation in the C-terminal region of spider silk proteins (spidroin) from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae), J. Biol. Chem. 269, 6661-6663.
10. Motriuk-Smith, D., Smith, A., Hayashi, C. Y., and Lewis, R. V. (2005) Analysis of the conserved N-terminal domains in major ampullate spider silk proteins, Biomacromolecules 6, 3152-3159.
11. Hu, X., Vasanthavada, K., Kohler, K., McNary, S., Moore, A. M., and Vierra, C. A. (2006) Molecular mechanisms of spider silk, Cell Mol. Life Sci. 63, 1986-1999.
12. Simmons, A., Ray, E., and Jelinski, L. W. (1994) Solid-state 13C NMR of Nephila clavipes dragline silk establishes structure and identity of crystalline regions, Macromolecules 27, 5235-5237.
13. Parkhe, A. D., Seeley, S. K., Gardner, K., Thompson, L., and Lewis, R. V. (1997) Structural studies of spider silk proteins in the fiber, J. Mol. Recognit. 10, 1-6.
14. van Beek, J. D., Hess, S., Vollrath, F., and Meier, B. H. (2002) The molecular structure of spider dragline silk: Folding and orientation of the protein backbone, Proc. Natl. Acad. Sci. U.S.A. 99, 10266-10271.
15. Hayashi, C. Y., Shipley, N. H., and Lewis, R. V. (1999) Hypotheses that correlate the sequence, structure, and mechanical properties of spider silk proteins, Int. J. Biol. Macromol. 24, 271-275.
16. Vollrath, F., Fairbrother, W. J., Williams, R. J. P., Tillinghast, E. K., Bernstein, D. T., Gallagher, K. S., and Townley, M. A. (1990) Compounds in the droplets of the orb spider's viscid spiral, Nature 345, 526-528.
17. Fischer, F. G., and Brander, J. (1960) An analysis of the web of the garden spider, Hoppe Seylers Z., Physiol. Chem. 320, 92-102.
18. Schildknecht, H., Kunzelmann, P., Kraub, D., and Kuhn, C. (1972) Uber die chemie der spinnwebe I: Anthropodenabwehrstoffe, LVII Naturwissenschaften 59, 98-99.
19. Higgins, L. E., Townley, M. A., Tillinghast, E. K., and Rankin, M. (2001) Variation in the chemical composition of orb webs built by the spider Nephila clavipes (Araneae, Tetragnathidae), J. Arachnol. 29, 82-94.
20. Tillinghast, E. K., Townley, M. A., Bernstein, D. T., Gallagher, K. S. (1991) Comparative study of orb web hygroscopicity and adhesive spiral composition in three araneid spiders, J. Exp. Zool. 259, 154-165.
21. Vollrath, F., and Edmonds, D. T. (1989) Modulation of the mechanical properties of spider silk by coating with water, Nature 340, 305-307.
22. Vollrath, F., and Tillinghast, E. K. (1991) Glycoprotein glue beneath a spider web's aqueous coat, Naturwissenschaften 78, 557-559.
23. Tillinghast, E. K., Townley, M. A., Wight, T. N., Uhlenbruck, G., and Janssen, E. (1993) Mater. Res. Soc. Symp. Proc. 9-23.
24. Hu, X., Kohler, K., Falick, A. M., Moore, A. M., Jones, P. R., Sparkman, O. D., and Vierra, C. (2005) Egg case protein-1. A new class of silk proteins with fibroin-like properties from the spider Latrodectus Hesperus, J. Biol. Chem. 280, 21220-21230.
25. Kohler, K., Thayer, W., Le, T., Sembhi, A., Vasanthavada, K., Moore, A. M., and Vierra, C. A. (2005) Characterization of a novel class II bHLH transcription factor from the black widow spider, Latrodectus hesperus, with silk-gland restricted patterns of expression, DNA Cell Biol. 24, 371-380.
26. Bendtsen, J. D., Nielsen, H., von Heijne, G., and Brunak, S. (2004) Improved prediction of signal peptides: SignalP 3.0, J. Mol. Biol. 340, 783-795.
27. Foradori, M. J., Kovoor, J., Moon, M. J., and Tillinghast, E. K. (2002) Relation between the outer cover of the egg case of Argiope aurantia (Araneae: Araneidae) and the emergence of its spiderlings, J. Morphol. 252, 218-226.
28. Hu, X., Kohler, K., Falick, A. M., Moore, A. M., Jones, P., and Vierra, C. (2006) Spider egg case core fibers: Trimeric complexes assembled from Tusp1, ECP-1 and ECP-2, Biochemistry 45, 3506-3516.
29. Arai, T., Freddi, G., Colonna, G. M., Soctti, E., Boschi, A., Murakami, R., and Tsukada, M. (2001) Absorption of metal cations by modified B. Mori silk and preparation of fabrics with antimicrobial activity, J. Appl. Polym. Sci. 80, 297-303.
30. Zhou, L., Chen, X., Shao, Z., Huang, Y., and Knight, D. P. (2005) Effect of metallic ions on silk formation in the mulberry silkworm, Bombyx mori, J. Phys. Chem. B 109, 16937-16945.
31. Dicko, C., Kenney, J. M., Knight, D., and Vollrath, F. (2004) Transition to a beta-sheet-rich structure in spidroin in vitro: The effects of pH and cations, Biochemistry 43, 14080-14087.
32. Argintean, S., Chen, J., Kim, M., and Moore, A. M. F. (2006) Resilient silk captures prey in black widow cobwebs, Appl. Phys. A: Mater. Sci. Process. 82, 235-241.
33. Liivak, O., Flores, A., Lewis, L., and Jelinski, L. W. (1997) Conformation of the polyalanine repeats in minor ampullate gland silk of the spider Nephila clavipes, Macromolecules 30, 7127-7130.