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Volumn 272, Issue 5258, 1996, Pages 112-115

Silk properties determined by gland-specific expression of a spider fibroin gene family

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; FIBROIN; GLYCINE;

EID: 0029925013     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5258.112     Document Type: Article
Times cited : (399)

References (21)
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    • F. Vollrath, Sci. Am. 266, 70 (March 1992); J M Gosline, M E. DeMont, M W. Denny, Endeavour 10, 37 (1986).
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    • J. F. V. Vincent and J. D. Currey, Eds. Cambridge Univ Press, Cambridge
    • M. Denny, in The Mechanical Properties of Biological Materials, J. F. V. Vincent and J. D. Currey, Eds. (Cambridge Univ Press, Cambridge, 1980). pp 475-559
    • (1980) The Mechanical Properties of Biological Materials , pp. 475-559
    • Denny, M.1
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    • M. Sankaya and I. Aksay, Eds. AIP Press, Woodberry, NY
    • J. M. Gosline et al., in Biomimetics, M. Sankaya and I. Aksay, Eds. (AIP Press, Woodberry, NY, 1995), pp. 237-261.
    • (1995) Biomimetics , pp. 237-261
    • Gosline, J.M.1
  • 7
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    • D Kaplan, W. Adams, B. Farmer, C. Viney, Eds. American Chemical Society, Washington, DC
    • J. M. Gosline et al., in Material Science and Biotechnology, D Kaplan, W. Adams, B. Farmer, C. Viney, Eds. (American Chemical Society, Washington, DC, 1994), pp. 328-341
    • (1994) Material Science and Biotechnology , pp. 328-341
    • Gosline, J.M.1
  • 10
    • 13344296273 scopus 로고    scopus 로고
    • note
    • Abbreviations for the amino acid residues are as follows. A, Ala, C, Cys; D, Asp; E, Glu; F, Phe; G, Gly, H His; I, lle; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser, T, Thr, V, Val; W, Trp; and Y, Tyr.
  • 12
    • 13344289996 scopus 로고    scopus 로고
    • note
    • 32P]deoxycytidine 5′-triphosphate (dCTP). 5′-GCAGCAGCAGCAGCAGCA-3′, 5′-GGACAAGGAGGATATGGAGGT-3′, and 5′-GGACCAGGACAACAAGGACCAGGA-3′. Twenty putative silk clones were chosen from each library, and pBluescript phagemids with cDNA inserts were excised in vivo into Escherichia coli SOLR cells with the filamentous Exassist helper phage (Stratagene). Four unique partial cDNAs (ADF-1, 1 2 kb, ADF-2, 1.0 kb; ADF-3, 2.0 kb, and ADF-4, 1.4 kb) were sequenced from nested deletions generated with the Erase-A-Base system (Promega)
  • 14
    • 13344296272 scopus 로고    scopus 로고
    • International Biotechnologies, New Haven, CT
    • Mac Vector, version 4.1.1 (International Biotechnologies, New Haven, CT).
    • Mac Vector, Version 4.1.1
  • 17
    • 13344252562 scopus 로고    scopus 로고
    • note
    • 32P]dCTP. These regions included the "unique" UTR sequence to minimize cross-hybridization to related transcripts.
  • 21
    • 13344292416 scopus 로고    scopus 로고
    • note
    • Supported by grants from Natural Sciences and Engineering Research Council of Canada and E I DuPont de Nemours & Co. to J M G We acknowledge lab space provided by M Hayden and critical review of the manuscript by H. Brock, T Grigliatti, M Lillie, and D Nelson


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.