Large scale production of Bacillus thuringiensis PS149B1 insecticidal proteins Cry34Ab1 and Cry35Ab1 from Pseudomonas fluorescens

Protein Expression and Purification

Volumn 53, Issue 2, 2007, Pages 325-330

  Huang, Ke xue ^a   Badger, Monty ^a   Haney, Keith ^a   Evans, Steve L ^a  

^a DOW AGROSCIENCES LLC   (United States)

Author keywords

Bacillus thuringiensis PS149B1; Cry34Ab1; Cry35Ab1; Pseudomonas fluorescens; Purification

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENE; BIOSYNTHESIS; CELL INCLUSION; CHEMISTRY; FERMENTATION; GENE EXPRESSION; GENETICS; INSECT; ISOLATION AND PURIFICATION; MAIZE; PARASITOLOGY; PATHOGENICITY; PLASMID; PSEUDOMONAS FLUORESCENS; TRANSGENIC PLANT;

ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; ENDOTOXINS; FERMENTATION; GENE EXPRESSION; GENES, BACTERIAL; HEMOLYSIN PROTEINS; INCLUSION BODIES; INSECTS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; ZEA MAYS;

BACILLUS THURINGIENSIS; DIABROTICA VIRGIFERA VIRGIFERA; PSEUDOMONAS FLUORESCENS; ZEA MAYS;

EID: 33947413954     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.01.010     Document Type: Article

References (29)

1. Metcalf R.L. Forward. In: Krysan J.L., and Miller T.A. (Eds). Methods for the study of the pest Diabrotica (1986), Springer-Verlag, New York vii-xv
2. Ellis R.T., Stockhoff B.A., Stamp L., Schnepf H.E., Schwab G.E., Knuth M., Russell J., Cardineau G.A., and Narva K.E. Appl. Environ. Microbiol. 68 (2002) 1137-1145
3. Zhang M., and Gill S.S. Mode of action of Bacillus thuringiensis toxins. In: Voss G., and Ramos G. (Eds). Chemistry of Crop Protection, Progress and Prospects in Science and Regulation (2002), Springer-Verlag, New York 213-236
4. Perlak F.J., Deaton R.W., Armstrong T.A., Fuchs R.L., Sims S.R., Greenplate J.T., and Fischhoff D.A. Insect resistance cotton plants. Biotechnology (NY) 8 (1990) 939-943
5. Perlak F.J., Stone T.B., Muskopf Y.M., Petersen L.J., Parker G.B., McPherson S.A., Wyman J., Love S., Reed G., Biever D., and Fischhoff D.A. Genetically improved potatoes: protection from damage by Colorado potato beetles. Plant Mol. Biol. 22 (1993) 313-321
6. Fujimoto H., Itoh K., Yamamoto M., Kyozuka J., and Shimamoto K. Insect resistance rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis. BioTechnology 11 (1993) 1151-1155
7. Koziel M.G., et al. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. BioTechnology 11 (1993) 194-200
8. Schnepf H.E., Lee S., Dojillo J., Burmeister P., Fencil K., Morera L., Nygaard L., Narva K.E., and Wolt J.D. Characterization of Cry34/Cry35 binary insecticidal proteins from diverse Bacillus thuringiensis strain collections. Appl. Environ. Microbiol. 71 (2005) 1765-1774
9. Herman R.A., Scherer P.N., Young D.L., Mihaliak C.A., Meade T., Woodsworth A.T., Stockhoff B.A., and Nava K.E. Binary insecticidal crystal protein from Bacillus thuringiensis strain PS149B1: effect of individual protein components and mixtures in laboratory bioassays. J. Econ. Entomol. 95 (2002) 635-639
10. Moellenbeck D.J., Peters M.L., Bing J.W., Rouse J.R., Higgins L.S., Sims L., Nevshemal T., Marshall L., Ellis R.T., Bystrak P.G., Lang B.A., Stewart J.L., Kouba K., Sondag V., Gustafson V., Nour K., Xu D.P., Swenson J., Zhang J., Czapla T., Schwab G., Jayne S., Stockhoff B.A., Narva K., Schnepf H.E., Stelman S.J., Poutre C., Koziel M., and Duck N. Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms. Nat. Biotechnol. 9 (2001) 668-672
11. Evans S.L. Producing proteins derived from genetically modified organism for toxicology and environmental fate assessment of biopesticides. In: Parekh S.R. (Ed). The GMO handbook: Genetically Modified Animals, Microbes, and Plants in Biotechnology (2004), Humana Press Inc., Totowa, NJ 53-83
12. Squires C.H., Retallack D.M., Chew L.C., Ramseier T.M., Scheider J.C., and Talbot H.W. Heterologous protein production in P. fluorescens. Bioprocess Int. 2 (2004) 54-59
13. Chew L.C., Ramseier T.M., Retallack D.M., Schneider J.C., Squires C.H., and Talbot H.W. Pseudomonas fluorescens. In: Gellissen G. (Ed). Production of Recombinant Proteins. Novel Microbial and Eucaryotic Systems (2005), WILEY-VCH verlag GmbH & Co. KGaA, Weinheim 45-65
14. EPA. M-One plus bioinsecticide; tolerance exemption. Fed. Regist. 56 (1991) 28325-28356
15. EPA. MVP bioinsecticide; tolerance exemption. Fed. Regist. 56 (1991) 28326-28328
16. EPA. Cry1A(c) and Cry1C derived delta endotoxins of Bacillus thuringensis encapsulated in killed Pseudomonas fluorescens; exemption from requirement of a tolerance. Fed. Regist. 60 (1995) 47487-47489
17. E.R. Wilcox, Methods, vectors and host cells for the control of expression of heterologous genes from lac operated promoters, US patent 5,169,760, 1992.
18. M. Thompson, D. Mar, G.E. Schwab, Delta-endotoxin expression in Pseudomonas fluorescens, U.S. Patent 5,527,883, 1996.
19. Riesenberg D., Schulz V., Knorre W.A., Pohl H.D., Korz D., Sanders E.A., Ross A., and Deckwer W.D. High density cultivation of Escherichia coli at controlled specific growth rate. J. Biotechnol. 20 (1991) 17-27
20. Gao Y., Schafer B.W., Collins R.A., Herman R.A., Xu X.P., Gilbert J.R., Ni W.T., Langer V.L., and Tagliani L.A. Characterization of Cry34Ab1 and Cry35Ab1 insecticidal crystal proteins expressed in transgenic corn plants and Pseudomonas fluorescens. J. Agric. Food Chem. 52 (2004) 8057-8065
21. Couch T.L., and Ross D.A. Production and utilization of Bacillus thuringiensis. Biotechnol. Bioeng. 22 (1980) 1297-1304
22. McLean K.M., and Whiteley H.R. Expression in Escherichia coliof a cloned crystal protein gene of Bacillus thuringiensis subsp.israelensis. J. Bacteriol. 169 (1987) 1017-1023
23. Chang C., Dai S.M., Frutos R., Frutos B.A., and Gill S.S. Properties of a 72-kilodalton mosquitocidal protein from Bacillus thuringiensis subsp. morrisoni PG-expressed in B. thuringiensis subsp. kurstaki by using the shuttle vector pHT3101. Appl. Environ. Microbiol. 58 (1992) 507-512
24. Obukowicz M.G., Perlak F.J., Kusano-Kretzmer K., Mayer E.J., and Watrud L.S. Integration of the delta endotoxin gene of Bacillus thuringiensis into the chromosome of root colonizing strain of pseudomonads using Tn5. Gene 45 (1986) 327-331
25. Boonserm P., Pornwiroon W., Katzenmeier G., Panyim S., and Angsuthanasombat C. Optimised expression in Escherichia coli and purification of the functional form of the Bacillus thuringiensis Cry4Aa delta-endotoxin. Protein Expr. Purif. 35 (2004) 397-403
26. Manasherob R., Itsko M., Sela-Baranes N., Ben-Dov E., Berry C., Cohen S., and Zaritsky A. Cyt1Ca from Bacillus thuringiensis subsp. israelensis: production in Escherichia coli and comparison of its biological activities with those of other Cyt-like proteins. Microbiology 152 (2006) 2651-2659
27. Promdonkoy B., Promdonkoy P., Audtho M., Tanapongpipat S., Chewawiwat N., Luxananil P., and Panyim S. Efficient expression of the mosquito larvicidal binary toxin gene from Bacillus sphaericus in Escherichia coli. Curr. Microbiol. 47 (2003) 383-387
28. Gustafson M.E., Clayton R.A., Lavrik P.B., Johnson G.V., Leimgruber R.M., Sims S.R., and Bartnicki D.E. Large-scale production and characterization of Bacillus thuringiensis subsp. tenebrionis insecticidal protein from Escherichia coli. Appl. Microbiol. Biotechnol. 47 (1997) 255-261
29. Bradshaw R.A., Brickey W.W., and Walker K.W. N-terminal processing: the methionine aminopeptidase and N-acetyl transferase familes. Trends Biochem. Sci. 23 (1998) 263-267