Degenerated primer design to amplify the heavy chain variable region from immunoglobulin cDNA

BMC Bioinformatics

Volumn 7, Issue SUPPL.4, 2006, Pages

  Wang, Ying ^a   Chen, Wei ^a   Li, Xu ^a   Cheng, Bing ^a  

^a XI'AN JIAOTONG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY GENES; CONSERVED REGIONS; GENE FAMILIES; GENE LIBRARIES; HYBRIDOMA CELLS; NEW PROTOCOL; PRIMER DESIGN; VARIABLE REGIONS;

ALGORITHMS; CELL CULTURE; CHAINS; CLONING; DESIGN;

GENES;

COMPLEMENTARY DNA; IMMUNOGLOBULIN HEAVY CHAIN; PRIMER DNA; IMMUNOGLOBULIN VARIABLE REGION;

AMINO ACID SEQUENCE; ARTICLE; CELL LINE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE IDENTIFICATION; GENE LIBRARY; GENETIC ALGORITHM; GENETIC CONSERVATION; HYBRIDOMA; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGICAL PROCEDURES; MOLECULAR CLONING; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; PROCESS DESIGN; PROCESS DEVELOPMENT; DNA SEQUENCE; GENE REARRANGEMENT; GENETICS; NUCLEIC ACID AMPLIFICATION; PHYSIOLOGY; PROCEDURES;

CELL LINE; DNA PRIMERS; DNA, COMPLEMENTARY; GENE REARRANGEMENT; HYBRIDOMAS; IMMUNOGLOBULIN VARIABLE REGION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 33947385831     PISSN: None     EISSN: 14712105     Source Type: Journal    
DOI: 10.1186/1471-2105-7-S4-S9     Document Type: Article

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