Optimizing the ratio of horseradish peroxidase and glucose oxidase on a bienzyme electrode: Comparison of a theoretical and experimental approach

Sensors and Actuators, B: Chemical

Volumn 122, Issue 2, 2007, Pages 395-402

  Mackey, Dana ^a   Killard, Anthony J ^b   Ambrosi, Adriano ^b   Smyth, Malcolm R ^b  

^a DUBLIN INSTITUTE OF TECHNOLOGY   (Ireland)

^b DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Biosensor; Horseradish peroxidase glucose oxidase electrode; Mathematical and computational modelling; Michaelis Menten kinetics

Indexed keywords

BOUNDARY CONDITIONS; COMPUTER SIMULATION; ELECTRODES; ENZYME KINETICS; MATHEMATICAL MODELS; NUMERICAL METHODS; PARTIAL DIFFERENTIAL EQUATIONS;

COMPUTATIONAL MODELING; GLUCOSE OXIDASE ELECTRODES; HORSERADISH PEROXIDASE; MICHAELIS-MENTEN KINETICS;

BIOSENSORS;

EID: 33947167814     PISSN: 09254005     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.snb.2006.06.006     Document Type: Article

References (8)

1. Berg J.M., Tymoczko J.L., and Stryer L. Biochemistry (2002), W.H. Freeman and Company, New York
2. Cambiaso A., Delfino L., Grattarola M., Verreschi G., Ashworth D., Maines A., and Vadgama P. Modelling and simulation of a diffusion limited glucose biosensor. Sens. Actuators B 33 (1996) 203-207
3. Dunford H.B. Heme Peroxidases (1999), Wiley-VCH, New York
4. Gorton L., Csöregi E., Ruzgas T., Gazaryan I., and Marko-Varga G. Enzyme biosensors based on electron transfer between electrode and immobilized peroxidases. In: Mulchandani A., and Rogers K.R. (Eds). Enzyme and Microbial Biosensors. Techniques and Protocols vol. 6 (1998), Humana Press, Totowa, NJ 93-120
5. Killard A.J., Zhang S., Zhao H., John R., Iwuoha E.I., and Smyth M.R. Development of an electrochemical flow-injection immunoassay (FIIA) for the real-time monitoring of biospecific interactions. Anal. Chim. Acta 400 (1999) 109-119
6. Iwuoha E.I., Leister I., Miland E., Smyth M.R., and Fágáin C.O. Reactivities of organic phase biosensors. 1. Enhancement of the sensitivity and stability of am-perometric peroxidase biosensors using chemically modified enzymes. Anal. Chem. 69 (1997) 1674-1681
7. Iwuoha E.I., Leister I., Miland E., Smyth M.R., and Fágáin C.O. Reactivities of organic phase biosensors. 2. The amperometric behaviour of horseradish peroxidase immobilised on a platinum electrode modified with an electrosynthetic polyaniline film. Bios. Biolectron. 12 (1997) 749-761
8. Morrin A., Weelber F., Ngamna O., Moulten S.E., Killard A.J., Wallace G.G., and Smyth M.R. Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles. Electrochem. Commun. 7 (2005) 317-322