Synip phosphorylation is required for insulin-stimulated Glut4 translocation

Biochemical and Biophysical Research Communications

Volumn 356, Issue 1, 2007, Pages 102-106

  Okada, Shuichi ^a   Ohshima, Kihachi ^b   Uehara, Yutaka ^a   Shimizu, Hiroyuki ^a   Hashimoto, Koshi ^a   Yamada, Masanobu ^a   Mori, Masatomo ^a  

^a GUNMA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b GUNMA UNIVERSITY   (Japan)

Author keywords

Akt2; Glucose transporter 4 (Glut4); Insulin; Synip; Syntaxin4

Indexed keywords

ALANINE; BETA GALACTOSIDASE; CELL PROTEIN; GLUCOSE TRANSPORTER 4; INSULIN; MUTANT PROTEIN; PHENYLALANINE; PROTEIN KINASE B; PROTEIN KINASE B BETA; PROTEIN SYNIP; SERINE; SYNTAXIN; SYNTAXIN 4; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CONSENSUS SEQUENCE; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVITY; GENE OVEREXPRESSION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION;

3T3-L1 CELLS; ADIPOCYTES; AMINO ACID SUBSTITUTION; ANIMALS; BETA-GALACTOSIDASE; BLOTTING, WESTERN; ELECTROPHORESIS; GLUCOSE TRANSPORTER TYPE 4; INSULIN; MICE; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN TRANSPORT; QA-SNARE PROTEINS; TRANSFECTION; VESICULAR TRANSPORT PROTEINS;

EID: 33947128563     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.02.095     Document Type: Article

References (16)

1. Chen W.S., Xu P.Z., Gottlob K., Chen M.L., Sokol K., Shiyanova T., Roninson I., Weng W., Suzuki R., Tobe K., Kadowaki T., and Hay N. Growth retardation and increased apoptosis in mice with homozygous disruption of the Akt1 gene. Genes Dev. 15 (2001) 2203-2208
2. Cho H., Thorvaldsen J.L., Chu Q., Feng F., and Birnbaum M.J. Akt1/PKB alpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice. J. Biol. Chem. 276 (2001) 38349-38352
3. Cho H., Mu J., Kim J.K., Thorvaldsen J.L., Chu Q., Crenshaw III E.B., Kaestner K.H., Bartolomei M.S., Shulman G.I., and Birnbaum M.J. Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science 292 (2001) 1728-1731
4. George S., Rochford J.J., Wolfrum C., Gray S.L., Schinner S., Wilson J.C., Soos M.A., Murgatroyd P.R., Williams R.M., Acerini C.L., Dunger D.B., Barford D., Umpleby A.M., Wareham N.J., Davies H.A., Schafer A.J., Stoffel M., O'Rahilly S., and Barroso I. A family with severe insulin resistance and diabetes due to a mutation in AKT2. Science 28 (2004) 1325-1328
5. Tschopp O., Yang Z.Z., Brodbeck D., Dummler B.A., Hemmings-Mieszczak M., Watanabe T., Michaelis T., Frahm J., and Hemmings B.A. Essential role of protein kinase B gamma (PKB gamma/Akt3) in postnatal brain development but not in glucose homeostasis. Development 132 (2005) 2943-2954
6. Chan T.O., Rittenhouse S.E., and Tsichlis P.N. AKT/PKB and other D3 phosphoinositide-regulated kinases: kinase activation by phosphoinositide-dependent phosphorylation. Annu. Rev. Biochem. 68 (1999) 965-1014
7. Bae S.S., Cho H., Mu J., and Birnbaum M.J. Isoform-specific regulation of insulin-dependent glucose uptake by Akt/protein kinase B. J. Biol. Chem. 278 (2003) 49530-49536
8. Zhen J.Y., Qiong Z.J., Kerri C.A., Chouinard My., Queta B., and Michael C.P. Insulin signaling through Akt/protein kinase B analyzed by small interfering RNA-mediated gene silencing. Proc. Natl. Acad. Sci. USA 100 (2003) 7569-7574
9. Mónica C.R., Carmen M., Hongzhi L., Amr K J.E., Morris B.J., and Paul P.F. Insulin increases the association of Akt-2 with Glut4-containing vesicles. J. Biol. Chem. 273 (1998) 7201-7204
10. Yamada E., Okada S., Saito T., Ohshima K., Sato M., Tsuchiya T., Uehara Y., Shimizu H., and Mori M. Akt2 phosphorylates Synip to regulate docking and fusion of GLUT4-containing vesicles. J. Cell Biol. 168 (2005) 921-928
11. Masure S., Haefner B., Wesselink J.J., Hoefnagel E., Mortier E., Verhasselt P., Tuytelaars A., Gordon R., and Richardson A. Molecular cloning, expression and characterization of the human serine/threonine kinase Akt-3. Eur. J. Biochem. 265 (1999) 353-360
12. Sano H., Kane S., Sano E., and Lienhard G.E. Synip phosphorylation does not regulate insulin-stimulated GLUT4 translocation. Biochem. Biophys. Res. Commun. 332 (2005) 880-884
13. Min J., Okada S., Kanzaki M., Elmendorf J.S., Coker K.J., Ceresa B.P., Syu L.J., Noda Y., Saltiel A.R., and Pessin J.E. Synip: a novel insulin-regulated syntaxin4-binding protein mediating GLUT4 translocation in adipocytes. Mol. Cell 3 (1999) 751-760
14. Okada S., Mori M., and Pessin J.E. Introduction of DNA into 3T3-L1 adipocytes by electroporation. Methods Mol. Med. 83 (2003) 93-96
15. Foster L.J., and Klip A. Mechanism and regulation of GLUT-4 vesicle fusion in muscle and fat cells. Am. J. Physiol. 279 (2000) C877-C890
16. Olson A.L., Knight J.B., and Pessin J.E. Syntaxin4, VAMP2, and/or VAMP3/cellubrevin are functional target membrane and vesicle SNAP receptors for insulin-stimulated GLUT4 translocation in adipocytes. Mol. Cell Biol. 17 (1997) 2425-2435