Leishmania mexicana: Identification and characterization of an aspartyl proteinase activity

Experimental Parasitology

Volumn 116, Issue 1, 2007, Pages 77-82

  Valdivieso, Elizabeth ^a   Dagger, Fracehuli ^a   Rascón, Ana ^a  

^a FACULTAD DE CIENCIAS   (Venezuela)

Author keywords

Aspartyl proteinase; benzoyl Arg Gly Phe Phe Leu 4 methoxy naphthylamide; Bz RGFFL 4M NA; DAN; diazo acetyl norleucinemethylester; HF; HIV; human fibrinogen; human immunodeficiency virus; Leishmania mexicana; SF; soluble fraction

Indexed keywords

ASPARTIC PROTEINASE; CATHEPSIN D; FIBRINOGEN;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBSTRATE; IN VITRO STUDY; LEISHMANIA MEXICANA; NONHUMAN; PRIORITY JOURNAL; PROMASTIGOTE; PROTEIN DEGRADATION;

ANIMALS; ASPARTIC ENDOPEPTIDASES; CATTLE; FIBRINOGEN; HUMANS; LEISHMANIA MEXICANA; MICE; NORLEUCINE; SUBSTRATE SPECIFICITY;

HUMAN IMMUNODEFICIENCY VIRUS; LEISHMANIA MEXICANA;

EID: 33947121179     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2006.10.006     Document Type: Article

References (30)

1. Alves C.R., Corte-Real S., Bourguignon S.C., Chaves C.S., and Saraiva E.M.B. Leishmania amazonensis: early proteinase activities during promastigote-amastigote differentiation in vitro. Experimental Parasitology 112 (2005) 63-65
2. Anson M.L. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. Journal General Physiology 23 (1938) 79-91
3. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry 72 (1976) 248-254
4. Brindley P.J., Kalinna B.H., Wong J.Y., Bogitsh B.J., King L.T., Smyth D.J., Verity C.K., Abbenante G., Brinkworth R.I., Fairlie D.P., Smythe M.L., Milburn P.J., Bielefeldt-Ohmann H., Zheng Y., and McManus D.P. Proteolysis of human hemoglobin by Schistosome cathepsin D. Molecular Biochemical Parasitology 112 (2001) 103-112
5. Brown A., Girod N., Billett E.E., and Pritchard D.I. Necator americanus (human hookworm) aspartyl proteinases and digestion of skin macromolecules during skin penetration. American Journal of Tropical Medicine and Hygiene 60 (1999) 840-847
6. Christeller J.T. Evolutionary mechanisms acting on proteinase inhibitor variability. FEBS Journal 272 (2005) 5710-5713
7. Coombs G.H., Hart D.T., and Capaldo J. Proteinase inhibitors as antileishmanial agents. Transactions of the Royal Society of Tropical Medicine and Hygiene 76 (1982) 660-663
8. Croft S.L., Vivas L., and Brooker S. Recent advances in research and control of malaria, leishmaniasis, trypanosomiasis and schistosomiasis. Eastern Mediterranean Health Journal 9 (2003) 518-533
9. Davies D.R. The structure and function of the aspartic proteinases. Annual Review of Biophysical Chemistry 19 (1990) 189-215
10. Delaney A., Williamson A., Brand A., Ashcom J., Varghese G., Goud G.N., and Hawdon J.M. Cloning and characterisation of an aspartyl protease inhibitor (API-1) from Ancylostoma hookworms. International Journal of Parasitology 35 (2005) 303-313
11. Englard S., and Seifter S. Precipitation techniques. Methods in Enzymology 182 (1990) 285-300
12. Giam C.Z., and Boros I. In vivo and in vitro autoprocessing of human immunodeficiency virus protease expressed in Escherichia coli. Journal of Biological Chemistry 263 (1988) 14617-14620
13. Hansen J., Billich S., Schulze T., Sukrow S., and Moelling K. Partial purification and substrate analysis of bacterially expressed HIV protease by means of monoclonal antibody. The EMBO Journal 7 (1988) 1785-1792
14. Kiso M. Design and synthesis of HIV protease inhibitors containing allophenylnorstatine as a transition-state mimic. Advances in Experimental Medicine and Biology 362 (1995) 413-423
15. Klemba M., and Goldberg D.E. Biological roles of proteases in parasitic protozoa. Annual Review of Biochemistry 71 (2002) 275-305
16. Marciniszyn J., Hartsuck J.A., and Tang J. Mode of Inhibition of acid proteases by pepstatin. Journal of Biological Chemistry 251 (1976) 7088-7094
17. Marciniszyn J., Hartsuck J.A., and Tang J. Pepstatin inhibition mechanism. Advances in Experimental Medicine and Biology 95 (1977) 199-210
18. Mckerrow J.H. Development of cysteine protease inhibitors as chemotherapy for parasitic diseases: insights on safety, target validation, and mechanism of action. International Journal of Parasitology 29 (1999) 833-837
19. Oda K., Sugitani M., Fukuhara K., and Murao S. Purification and properties of a pepstatin insensitive carboxyl proteinase from a gram-negative bacterium. Biochimica et Biophysica Acta 923 (1987) 463-469
20. Rajagopalan T.G., Stein W.H., and Moore S. The inactivation of pepsin by diazoacetyl-norleucine methyl ester. Journal of Biological Chemistry 241 (1966) 4295-4297
21. Ramirez J.L., and Guevara P. The ribosomal gene spacer as a tool for the taxonomy of Leishmania. Molecular and Biochemical Parasitology 22 (1987) 177-183
22. Ruchel R. Proteinase. In: Bennett J.E., Hay R.J., and Peterson P.K. (Eds). New Strategies in Fungal Disease (1992), Churchill Livingstone, Edinburg 17-31
23. Sato K., Nagai Y., and Suzuki M. Purification and partial characterization of an acid proteinase from Dirofilaria immitis. Molecular and Biochemical Parasitology 58 (1993) 293-300
24. Schägger H., and von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDA. Analytical Biochemistry 166 (1987) 368-379
25. Scharpe S., De Meester I., Hendriks P., Vanhoof G., van Sande M., and Vriend G. Proteases and their inhibitors: today and tomorrow. Biochimie 73 (1991) 121-126
26. Seelmeier S., Schmidt H., Turk V., and von der Helm K. Human immunodeficiency virus has an aspartic-type protease that can be inhibited by pepstatin A. Proceedings of the National Academy of Sciences of the United States of America 85 (1988) 6612-6616
27. Silva F.P., Ribeiro F., Katz N., and Giovanni-De-Simone S. Exploring the subsite specificity of Schistosoma mansoni aspartyl hemoglobinase though comparative molecular modeling. FEBS Letters 514 (2002) 141-148
28. Smith R.E., and Van Frank R.M. The use of amino acid derivatives of 4-methoxy-beta-naphthylamine for assay and subcellular localization of tissue proteinases. Frontiers in Bioscience 43 (1975) 193-249
29. Toogood H.S., Prescott M., and Daniel R.M. A pepstatin-insensitive aspartic proteinase from a thermophilic Bacillus sp. Biochemical Journal 307 (1995) 783-789
30. Travis J., Potempa J., and Maeda H. Are bacterial proteinases pathogenic factors?. Trends Microbiology 3 (1995) 405-407