Development of a real-time PCR for the identification of Bordetella pertussis and Bordetella parapertussis

Clinical Microbiology and Infection

Volumn 13, Issue 4, 2007, Pages 419-423

  Ménard, Armelle ^a,b   Lehours, P ^a   Sarlangue, J ^a   Bebear C ^a   Megraud F ^a   De Barbeyrac, B ^a  

^a HÔPITAL PELLEGRIN   (France)

^b INSERM   (France)

Author keywords

Bordetella; Diagnosis; Identification; Pertussis; Real time PCR

Indexed keywords

BACTERIAL DNA; JOSAMYCIN; MACROLIDE; PERTUSSIS VACCINE; RNA 16S;

ADULT; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BORDETELLA PARAPERTUSSIS; BORDETELLA PERTUSSIS; CLINICAL ARTICLE; CONTROLLED STUDY; FATALITY; FEMALE; GENE AMPLIFICATION; HUMAN; INFANT; MALE; MELTING POINT; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; PERTUSSIS; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SPECIES DIFFERENCE; SPECIES IDENTIFICATION; STRAIN DIFFERENCE; TEMPERATURE MEASUREMENT; VACCINATION;

BORDETELLA; BORDETELLA PARAPERTUSSIS; BORDETELLA PERTUSSIS;

EID: 33847774490     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2006.01659.x     Document Type: Article

References (22)

1. Laboratoires de bactériologie et pédiatriques hospitaliers Institut Pasteur Paris. RENACOQ: Surveillance de la coqueluche à l'hopital en 2000. In: Surveillance nationale des maladies infectieuses 1998-2000. Paris: Institut de Veille Sanitaire, 2003; 81-84.
2. Meade BD, Bollen A. Recommendations for use of the polymerase chain reaction in the diagnosis of Bordetella pertussis infections. J Med Microbiol 1994; 41: 51-55.
3. Riffelmann M, Wirsing von Konig CH, Caro V et.al. Nucleic acid amplification tests for diagnosis of Bordetella infections. J Clin Microbiol 2005; 43: 4925-4929.
4. Reischl U, Lehn N, Sanden GN, Loeffelholz MJ. Real-time PCR assay targeting IS 481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii. J Clin Microbiol 2001; 39: 1963-1966.
5. Kosters K, Riffelmann M, Wirsing von Konig CH. Real-time LightCycler PCR for detecting and discrimination of Bordetella pertussis and Bordetella parapertussis. J Med Microbiol 2002; 40: 1719-1722.
6. Makinen J, Viljanen MK, Mertsola J et.al. Evaluation of a real-time PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samples. Emerg Infect Dis 2001; 50: 436-440.
7. Sloan LM, Hopkins MK, Mitchell PS et.al. Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens. J Clin Microbiol 2002; 40: 96-100.
8. Cloud JL, Hymas WC, Turlak A et.al. Rapid typing of Bordetella pertussis pertussis toxin gene variants by LightCycler real-time PCR and fluorescence resonance energy transfer hybridization probe melting curve analysis. Diagn Microbiol Infect Dis 2002; 40: 2213-2216.
9. Templeton KE, Scheltinga SA, van der Zee A et.al. Comparison of real-time PCR and conventional hemi-nested PCR for the detection of Bordetella pertussis in nasopharyngeal samples. J Clin Microbiol 2003; 9: 746-749.
10. Poddar SK. Detection and discrimination of B. pertussis and B. holmesii by real-time PCR targeting IS 481 using a beacon probe and probe-target melting analysis. Mol Cell Probes 2003; 17: 91-98.
11. Poddar SK. Rapid detection of Bordetella pertussis by real-time PCR using SYBR green I and a LightCycler instrument. J Clin Lab Anal 2004; 18: 265-270.
12. Makinen J, Mertsola J, Viljanen MK et.al. Real-time LightCycler PCR for detection and discrimination of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol 2002; 40: 1719-1722.
13. Storm M, Advani A, Pettersson M et.al. Comparison of real-time PCR and pyrosequencing for typing Bordetella pertussis toxin subunit 1 variants. J Microbiol Meth 2005; 19: 329-333.
14. Sloan LM, Hopkins MK, Mitchell PS et.al. Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis. J Clin Microbiol 2001; 7: 952-958.
15. Poddar SK. Differential detection of B. pertussis from B. parapertussis using a polymerase chain reaction (PCR) in presence of SYBR green 1 and amplicon melting analysis. Mol Cell Probes 2004; 18: 429-435.
16. Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 1988; 16: 10881-10890.
17. Muyldermans G, Soetens O, Antoine M et.al. External quality assessment for molecular detection of Bordetella pertussis in European laboratories. J Clin Microbiol 2005; 43: 30-35.
18. Dworkin MS, Sullivan PS, Buskin SE et.al. Bordetella bronchiseptica infection in human immunodeficiency virus-infected patients. Clin Infect Dis 1999; 28: 1095-1099.
19. Ner Z, Ross LA, Horn MV et.al. Bordetella bronchiseptica infection in pediatric lung transplant recipients. Pediatr Transplant 2003; 7: 413-417.
20. Conseil supérieur d'hygiène publique de France. Calendrier vaccinal 2005 et autres avis du Conseil supérieur d'hygiène publique en France relatifs àla vaccination. Bull Epidemiol Hebd 2006; 29-30: 211-216.
21. van Soolingen D, Hermans PW, de Haas PE et.al. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol 1991; 29: 2578-2586.
22. Loeffelholz MJ, Thompson CJ, Long KS et.al. Detection of Bordetella holmesii using Bordetella pertussis IS 481 PCR assay. J Clin Microbiol 2000; 38: 467.