Subcellular imaging of isotopically labeled carbon compounds in a biological sample by ion microprobe (NanoSIMS)

Microscopy Research and Technique

Volumn 70, Issue 3, 2007, Pages 220-229

  Clode, Peta L ^a   Stern, Richard A ^a,b   Marshall, Alan T ^c  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b GEOSCIENCE AUSTRALIA   (Australia)

^c LA TROBE UNIVERSITY   (Australia)

Author keywords

Ion imaging; Isotopic tracers; Molecular transport; Secondary ion mass spectrometry

Indexed keywords

INORGANIC COMPOUNDS; ION BEAMS; IONS; ISOTOPES; SECONDARY EMISSION; SODIUM COMPOUNDS;

BIOLOGICAL SAMPLES; CARBON COMPOUNDS; ION IMAGING; ION MICROPROBE; ISOTOPIC TRACER; ISOTOPICALLY LABELED; MOLECULAR TRANSPORT; NANOSIMS; SECONDARY ION-MASS SPECTROMETRY; SUB-CELLULAR;

SECONDARY ION MASS SPECTROMETRY;

CARBON; CARBON 13; CESIUM; HALOGEN; INORGANIC COMPOUND; NITROGEN; ORGANIC COMPOUND; OXYGEN; PHOSPHORUS; RESIN; SULFUR;

ANALYTIC METHOD; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CORAL; ISOTOPE LABELING; MASS SPECTROMETRY; MATERIALS; NANOSCALE SECONDARY ION MASS SPECTROMETRY; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; RELIABILITY;

ANTHOZOA; GALAXEA FASCICULARIS;

EID: 33847765447     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20409     Document Type: Article

References (20)

1. Bénazet-Tambutté S, Allemand D, Jaubert J. 1996. Permeability of the oral epithelial layers in cnidarians. Mar Biol 126:43-53.
2. Brenna JT, Morrison GH. 1986. Ionization probability variations due to matrix in ion microscopic analysis of plastic-embedded and ashed biological specimens. Anal Chem 58:1675-1680.
3. Chandra S, Ausserer WA, Morrison GH. 1987. Evaluation of matrix effects in ion microscopic analysis of freeze-fractured, freeze-dried cultured cells. J Microsc 148:223-239.
4. Chandra S, Sod EW, Ausserer WA, Morrison GH. 1992. Preparation of biological samples for ion microscopy. Pure Appl Chem 64:254-262.
5. Chandra S, Smith DR, Morrison GH. 2000. Subcellular imaging by dynamic SIMS ion microscopy. Anal Chem 1:104A-114A.
6. Clerc J, Fourre C, Fragu P. 1997. SIMS microscopy: Methodology, problems and perspectives in mapping drugs and nuclear medicine compounds. Cell Biol Int 21:619-633.
7. Clode PL, Marshall AT. 2002. Low temperature FESEM of the calcifying interface of a scleractinian coral. Tissue Cell 34:181-191.
8. Clode PL, Stern R, Griffin BJ. 2004. NanoSIMS: A new analytical tool for the biomedical and life sciences. In: Proceedings of 8th Asia-Pacific Conference on Electron Microscopy, Kanazawa, Japan, 7-11 June. p. 42.
9. Coplen TB, Hopple JA, Bo'hlke JK, Peiser HS, Rieder SE, Krouse HR, Rosman KJR, Ding T, Vocke RD, Jr, Révész KM, Lamberty A, Taylor P, De Bièvre P. 2002. Compilation of minimum and maximum isotope ratios of selected elements in naturally occurring terrestrial materials and reagents. In: United States geological survey water-resources investigations report 01-4222. Reston, VA: United States Geological Survey.
10. Echlin P. 1992. Low temperature microscopy and analysis. New York: Plenum Press.
11. Gillen G, Bright D. 2003. Tools and procedures for quantitative microbeam isotope ratio imaging by secondary ion mass spectrometry. Scanning 25:165-174.
12. Goldsmith JG, Lazof DB, Linton RW. 1999. Secondary ion mass spectrometry. In: Ingram P, Shelburne J, Rogglib V, LeFurgey A, editors. Biomedical applications of microprobe analysis. London: Academic Press. Ch. 15, pp. 461-508.
13. Guerquin-Kern JL, Hillion F, Madelmont J-C, Labarre P, Papon J, Croisy A. 2004. Ultra-structural cell distribution of the melanoma marker iodobenzamide: Improved potentiality of SIMS imaging in life sciences. Biomed Eng 3:1-7.
14. Hallegot P, Peteranderl R, Lechene C. 2004. In-situ imaging mass spectrometry analysis of melanin granules in the human hair shaft. J Invest Dermatol 122:381-386.
15. Kleinfeld AM, Kampf JP, Lechene C. 2004. Transport of C-13-oleate in adipocytes measured using multi imaging mass Spectrometry. J Am Soc Mass Spectrom 15:1572-1580.
16. Ludwig KR. 2003. User's manual for Isoplot 3.00: A geochronological toolkit for Microsoft Excel. Berkeley, CA: Berkeley Geochronology Center. Special publication 4.
17. Marshall AT, Wright OP. 1991. Freeze-substitution of scleractinian coral for confocal scanning laser microscopy and X-ray microanalysis. J Microsc 162:341-354.
18. Meibom A, Cuif J-P, Hillion F, Constantz BR, Juillet-Leclerc A, Dauphin Y, Watanabe T, Dunbar RB. 2004. Distribution of Mg in coral skeleton. Geophys Res Lett 31:L23306.
19. Peteranderl R, Lechene C. 2004. Measure of carbon and nitrogen stable isotope ratios in cultured cells. J Am Soc Mass Spectrom 15: 478-485.
20. Slodzian G, Daigne B, Girard F, Boust F, Hillion F. 1992. Scanning secondary ion analytical microscopy with parallel detection. Biol Cell 74:53-50.