High-throughput liberation of water-soluble yeast content by irreversible electropermeation (HT-irEP)

Journal of Biomolecular Screening

Volumn 12, Issue 2, 2007, Pages 267-275

  Zakhartsev, Maxim ^a,c   Momeu, Carmen ^a   Ganeva, Valentina ^b  

^a JACOBS UNIVERSITY BREMEN   (Germany)

^b UNIVERSITY OF SOFIA   (Bulgaria)

^c ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESEARCH   (Germany)

Author keywords

High throughput screening; Membrane electropermeation; Plasmid liberation; Protein liberation; Yeast library

Indexed keywords

RECOMBINANT PROTEIN; WATER;

ARTICLE; AUTOMATION; CHEMICAL ANALYSIS; DNA LIBRARY; DNA VECTOR; ELECTROPORATION; ENZYME ACTIVITY; ENZYME ASSAY; GENE AMPLIFICATION; HIGH THROUGHPUT SCREENING; IRREVERSIBLE ELECTROPERMEATION; MEMBRANE TRANSPORT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SOLUBILITY; YEAST;

CELL MEMBRANE PERMEABILITY; DNA, FUNGAL; ELECTROPORATION; GENE EXPRESSION; GENETIC VECTORS; GLUCOSE OXIDASE; GREEN FLUORESCENT PROTEINS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SOLUBILITY; SPECTROPHOTOMETRY; TRANSFORMATION, GENETIC; WATER;

SACCHAROMYCES CEREVISIAE;

EID: 33847685286     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057106296910     Document Type: Article

References (21)

1. Schutle H, Kula M-P: Pilot and process-scale techniques for cell disruption. Biotechnol Appl Biochem 1990 ; 12: 559 - 620.
2. Naglak TJ, Hettwer DJ, Wang HJ: Chemical permeabilization of cells for intracellular product release. Bioprocess Technol 1990 ; 9: 177 - 205.
3. Brockmeier U, Wendorff M, Eggert T: Versatile expression and secretion vectors for Bacillus subtilis. Curr Microbiol 2006 ; 52: 143 - 148.
4. Braun P, Gerritset G, van Dijl J-M, Quax WJ: Improving protein secretion by engineering components of the bacterial translocation machinery. Curr Opin Biotechnol 1999 ; 10: 376 - 381.
5. Zhu Z, Momeu C, Zakhartsev MV, Schwaneberg U: Making glucose oxidase fit for biofuel cell applications by directed protein evolution. Biosens Bioelectron 2006 ; 21: 2046 - 2051.
6. Weaver JC: Electroporation theory: concepts and mechanisms. Methods Mol Biol 1995 ; 47: 1 - 26.
7. Neumann E, Sprafke A, Boldth E, Walf H: Biophysical consideration of membrane electroporation. In Chang DC, Chassy JA, Saunders JA, Sower AE (eds): Guide to Electroporation and Electrofusion. San Diego, CA: Academic Press, 1992.
8. Teissie J, Ganeva V, Galutzov B: Electropulsing process for producing proteins. 10/419,188, US 2004/0097715A1: United States; 2004.
9. Ganeva V, Galutzov B, Teissie J: High yield electroextraction of proteins from yeast by a flow process. Anal Biochem 2003 ; 315: 77 - 84.
10. Ganeva V, Galutzov B: Electropulsation as an alternative method for protein extraction from yeast. FEMS Microbiol Lett 1999 ; 174: 279 - 284.
11. Ganeva V, Galutzov B, Eynard N, Teissie J: Electroinduced extraction of β-galactosidase from Kluyveromyces lactis. Appl Microbiol Biotechnol 2001 ; 56: 411 - 413.
12. Ganeva V, Galutzov B, Teissie J: Electroinduced release of invertase from Saccharomyces cerevisiae. Biotechnol Lett 2002 ; 24: 1853 - 1856.
13. Ganeva V, Galutzov B, Teissie J: Flow process for electroextraction of intracellular enzymes from the fission yeast Schizosaccharomyces pombe. Biotechnol Lett 2004 ; 26: 933 - 937.
14. Gietz RD, Schiestl RH, Willems AR, Wood RA: Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 1995 ; 11: 355 - 360.
15. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001.
16. Zakhartsev MV, Ganeva V: High throughput electropermeation system. EP06110993.0, DE; 2006.
17. Zlotnik H, Fernandes MP, Blowers B, Cabib E: Increased cell wall porosity in Saccharomyces cerevisiae after treatment with dithiothreitol or EDTA. J Gen Microbiol 1984 ; 135: 2077 - 2084.
18. Buhner M, Sund H: Yeast alcohol dehydrogenase: SH groups, disulfide groups, quaternary structure and reactivation by reducing cleavage of disulfide groups. Eur J Biochem 1969 ; 11: 73 - 79.
19. Hohmann S, Mager WH: Yeast Stress Responses. Berlin: Springer, 2003.
20. Nacken V, Degryse E, Achstetter T. Direct plasmid shuttling from yeast into E. coli by electroduction. Nucleic Acids Res 1994 ; 22: 1509 - 1510.
21. Janzen WP: High Throughput Screening: Methods and Protocols. Totowa, NJ: Humana, 2002.