Type III secretion system-associated pilus of Pseudomonas syringae as an epitope display tool

FEMS Microbiology Letters

Volumn 269, Issue 1, 2007, Pages 104-109

  Li, Chun Mei ^a   Hienonen, Elina ^a   Haapalainen, Minna ^a   Kontinen, Vesa P ^b   Romantschuk, Martin ^a   Taira, Suvi ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

Epitope display; Hrp pilus; HrpA; Type III secretion system (TTSS)

Indexed keywords

EPITOPE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM PILUS; CELL SECRETION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS SYRINGAE; SURFACE PROPERTY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; EPITOPES; FIMBRIAE, BACTERIAL; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PSEUDOMONAS SYRINGAE; VIRULENCE FACTORS;

PSEUDOMONAS SYRINGAE;

EID: 33847623104     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00612.x     Document Type: Article

References (15)

1. Boureau T, Routtu J, Roine E, Taira S & Romantschuk M (2002) Localization of hrpA induced Pseudomonas syringae pv. tomato DC3000 in infected tomato leaves. Mol Plant Pathol 3: 451-460.
2. Cuppels D (1986) Generation and characterization of Tn5 insertion mutations in Pseudomonas syringae pv. tomato. Appl Environ Microbiol 51: 323-327.
3. Galan JE & Collmer A (1999) Type III secretion machines: bacterial devices for protein delivery into host cells. Science 284: 1322-1328.
4. Hienonen E, Roine E, Romantschuk M & Taira S (2002) mRNA stability and the secretion signal of HrpA, a pilin secreted by the type III system in Pseudomonas syringae. Mol Genet Genomics 266: 973-978.
5. Huiskonen JT, Laakkonen L, Toropainen M, Sarvas M, Bamford DH & Bamford JK (2003) Probing the ability of the coat and vertex protein of the membrane-containing bacteriophage PRD1 to display meningococcal epitope. Virology 310: 267-279.
6. Huynh TV, Dahlbeck D & Staskawicz BJ (1989) Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity. Science 245: 1374-1377.
7. King EO, Ward MK & Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44: 301-307.
8. Klemm P & Schembri MA (2000) Fimbrial surface display systems in bacteria: from vaccines to random libraries. Microbiology 146: 3025-3032.
9. Li CM, Brown I, Mansfield J, Stevens C, Boureau T, Romantschuk M & Taira S (2002) The Hrp pilus of Pseudomonas syringae elongates from its tip and acts as a conduit for translocation of the effector protein HrpZ. EMBO J 21: 1909-1915.
10. Roine E, Saarinen J, Kalkkinen N & Romantschuk M (1997a) Purified HrpA of Pseudomonas syringae pv. tomato DC3000 reassembles into pili. FEBS Lett 417: 168-172.
11. Roine E, Wei W, Yuan J, Nurmiaho-Lassila EL, Kalkkinen N, Romantschuk M & He SY (1997b) Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. Proc Natl Acad Sci USA 94: 3459-3464.
12. Sambrook J, Fritsch E & Maniatis T (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Stebbins CE & Galan JE (2001) Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion. Nature 414: 77-81.
14. Taira S, Tuimala J, Roine E, Nurmiaho-Lassila EL, Savilahti H & Romantschuk M (1999) Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit. Mol Microbiol 34: 737-744.
15. Yang F, Moss LG & Phillips GN Jr (1996) The molecular structure of green fluorescent protein. Nature Biotechnol 14: 1246-1251.