Expression of functional human glutaminase in baculovirus system: Affinity purification, kinetic and molecular characterization

International Journal of Biochemistry and Cell Biology

Volumn 39, Issue 4, 2007, Pages 765-773

  Campos Sandoval, José A ^a   López de la Oliva, Amada R ^a   Lobo, Carolina ^a   Segura, Juan A ^a   Matés, José M ^a   Alonso, Francisco J ^a   Márquez, Javier ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

Author keywords

Glutamate; Glutamine; Human isozymes; Mitochondria; Nucleus; Recombinant protein expression

Indexed keywords

GLUTAMINASE;

ARTICLE; BACULOVIRUS; BINDING AFFINITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; HYDROLYSIS; IMMUNOAFFINITY CHROMATOGRAPHY; KIDNEY; KINETICS; MITOCHONDRION; MOLECULAR DYNAMICS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PURIFICATION; RECEPTOR BINDING; STOICHIOMETRY;

AMMONIA; ANIMALS; BACULOVIRIDAE; CATALYSIS; CELL LINE; CELL NUCLEUS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; GLUTAMIC ACID; GLUTAMINASE; GLUTAMINE; HUMANS; ISOENZYMES; KINETICS; MITOCHONDRIA; RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION;

HEXAPODA; MAMMALIA;

EID: 33847617845     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biocel.2006.12.002     Document Type: Article

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