A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli

Journal of Microbiological Methods

Volumn 68, Issue 3, 2007, Pages 497-506

  Liang, Rubing ^a   Liu, Xipeng ^a   Liu, Jianhua ^a   Ren, Qiushi ^a   Liang, Peiji ^a   Lin, Zhixin ^a   Xie, Xiangming ^b  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b BEIJING FORESTRY UNIVERSITY   (China)

Author keywords

Escherichia coli; Intein; T7 RNA polymerase; TS mutants; TS phenotype

Indexed keywords

ADENOSINE TRIPHOSPHATASE;

ARTICLE; ENZYMATIC ASSAY; ESCHERICHIA COLI; GENE TARGETING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE SENSITIVE MUTANT; TEMPERATURE SENSITIVITY; WESTERN BLOTTING;

DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; INTEINS; LAC OPERON; MICROBIOLOGICAL TECHNIQUES; MUTATION; PHENOTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE; VIRAL PROTEINS;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 33847334685     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2006.10.016     Document Type: Article

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