Mild surfection of neural cells, especially motoneurons, in primary culture and cell lines

Experimental Neurology

Volumn 204, Issue 1, 2007, Pages 118-130

  Rakotoarivelo, Clovis ^a   Petite, Didier ^a   de Weille, Jan ^a   Lumbroso, Serge ^b   Privat, Alain ^a   Sultan, Charles ^c   Mersel, Marcel ^a  

^a HÔPITAL SAINT ELOI   (France)

^b DEPARTMENT OF NEUROLOGY   (France)

^c HÔPITAL ARNAUD DE VILLENEUVE   (France)

Author keywords

Cationic liposome; Cholesterol; Cortical neurons; DOPE; DOTAP; GFP; Glial cells; Lipoplex coated coverslip; Primary motoneurons; Surfection

Indexed keywords

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; CHOLESTEROL; GREEN FLUORESCENT PROTEIN; LIPOFECTAMINE; PHOSPHATIDYLCHOLINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CELL MATURATION; CELL SURVIVAL; CELL TYPE; CONTROLLED STUDY; GLIA CELL; HELA CELL; HUMAN; HUMAN CELL; LABORATORY TEST; MOTONEURON; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CELL LINE; CELLS, CULTURED; CEREBRAL CORTEX; DNA; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; IMMUNOHISTOCHEMISTRY; LIPIDS; LIPOSOMES; MOTOR NEURONS; NEUROGLIA; NEURONS; PLASMIDS; RATS; RATS, SPRAGUE-DAWLEY; TRANSFECTION;

EID: 33847280410     PISSN: 00144886     EISSN: 10902430     Source Type: Journal    
DOI: 10.1016/j.expneurol.2006.09.027     Document Type: Article

References (31)

1. Arce V., Garces A., de Bovis B., Filippi P., Henderson C., Pettmann B., and deLapeyriere O. Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneurons purified by a novel technique. J. Neurosci. Res. 55 (1999) 119-126
2. Bielinska A.U., Yen A., Wu H.L., Zahos K.M., Sun R., Weine N.D., Baker Jr. J.R., and Roessler B.J. Application of membrane-based dendrimer/DNA complexes for solid phase transfection in vitro and in vivo. Biomaterials 21 (2000) 877-887
3. Camu W., and Henderson C.E. Purification of embryonic rat motoneurons by panning on a monoclonal antibody to the low-affinity NGF receptor. J. Neurosci. Methods 44 (1992) 59-70
4. Chalfie M., Tu Y., Euskirchen G., Ward W.W., and Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-805
5. Chang F.H., Lee C.H., Chen M.T., Kuo C.C., Chiang Y.L., Hang C.Y., and Roffler S. Surfection: a new platform for transfected cell arrays. Nucleic Acids Res. 32 (2004) e33
6. Cisterni C., Henderson C.E., Aebischer P., Pettmann B., and Deglon N. Efficient gene transfer and expression of biologically active glial cell line-derived neurotrophic factor in rat motoneurons transduced with lentiviral vectors. J. Neurochem. 74 (2000) 1820-1828
7. Crook K., Stevenson B.J., Dubouchet M., and Porteous D.J. Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum. Gene Ther. 5 (1998) 137-143
8. Da Cruz M.T., Simoes S., and de Lima M.C. Improving lipoplex-mediated gene transfer into C6 glioma cells and primary neurons. Exp. Neurol. 187 (2004) 65-75
9. Even-Chen S., and Barenholz Y. DOTAP cationic liposomes prefer relaxed over supercoiled plasmids. Biochim. Biophys. Acta 1509 (2000) 176-188
10. Farhood H., Serbina N., and Huang L. The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer. Biochim. Biophys. Acta 1235 (1995) 289-295
11. Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M., Northrop J.P., Ringold G.M., and Danielsen M. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. U. S. A. 84 (1987) 7413-7417
12. Freshney R. Culture of Animal Cells: A Manual of Basic Technique (1987), Alan R. Liss Inc., New York 117
13. Green B., and Leake R.E. Steroid Hormones: A Practical Approach (1987), IRL Press, Oxford 213-214
14. Gao X., and Huang L. Cationic liposome-mediated gene transfer. Gene Ther. 2 (1995) 710-722 (Review)
15. Haastert K., Grosskreutz J., Jaeckel M., Laderer C., Bufler J., Grothe C., and Claus P. Rat embryonic motoneurons in long-term co-culture with Schwann cells-A system to investigate motoneuron diseases on a cellular level in vitro. J. Neurosci. Methods. 142 (2005) 275-284
16. Heim R., Cubitt A.B., and Tsien R.Y. Improved green fluorescence. Nature 373 (1995) 663-664
17. Hofland H.E., Shephard L., and Sullivan S.M. Formation of stable cationic lipid/DNA complexes for gene transfer. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 7305-7309
18. Li S., and Huang L. In vivo gene transfer via intravenous administration of cationic lipid-protamine-DNA (LPD) complexes. Gene Ther. 4 (1997) 891-900
19. Lieberman A.P., Harmison G., Strand A.D., Olson J.M., and Fischbeck K.H. Altered transcriptional regulation in cells expressing the expanded polyglutamine androgen receptor. Hum. Mol. Genet. 11 (2002) 1967-1976
20. Liu Y., Peterson D.A., Kimura H., and Schubert D. Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. J. Neurochem. 69 (1997) 581-593
21. Maruyama K., Kennel S.J., and Huang L. Lipid composition is important for highly efficient target binding and retention of immunoliposomes. Proc. Natl. Acad. Sci. U. S. A. 87 (1990) 5744-5748
22. Neuhuber B., and Daniels M.P. Targeting of recombinant agrin to axonal growth cones. Mol. Cell. Neurosci. 24 (2003) 1180-1196
23. Petite D., and Calvet M.C. Cryopreserved neuronal cells in long-term cultures of dissociated rat cerebral cortex: survival and morphometric characteristics as revealed by immunocytochemistry. Brain Res. 669 (1995) 263-274
24. Rakotoarivelo C., Petite D., Lambard S., Fabre C., Rouleau C., Lumbroso S., de Weille J., Privat A., Carreau S., and Mersel M. Receptors to steroid hormones and aromatase are expressed by cultured motoneurons but not by glial cells derived from rat embryo spinal cord. Neuroendocrinology 80 (2004) 284-297
25. Ricart K.J., Pearson Jr. R., Viera L., Cassina P., Kamaid A., Carroll S.L., and Estevez A.G. Interactions between beta-neuregulin and neurotrophins in motor neuron apoptosis. J. Neurochem. 97 (2006) 222-233
26. Shea L.D., Smiley E., Bonadio J., and Mooney D.J. DNA delivery from polymer matrices for tissue engineering. Nat. Biotechnol. 17 (1999) 551-554 (Erratum in: Nat. Biotechnol. 17 (1999) 817)
27. Tang G.P., Zeng J.M., Gao S.J., Ma Y.X., Shi L., Li Y., Too H.P., and Wang S. Polyethylene glycol modified polyethylenimine for improved CNS gene transfer: effects of PEGylation extent. Biomaterials 24 (2003) 2351-2362
28. Thomas M., and Klibanov A.M. Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 14640-14645
29. Yang J.P., and Huang L. Overcoming the inhibitory effect of serum on lipofection by increasing the charge ratio of cationic liposome to DNA. Gene Ther. 4 (1997) 950-960
30. Zheng J., Manuel W.S., and Hornsby P.J. Transfection of cells mediated by biodegradable polymer materials with surface-bound polyethyleneimine. Biotechnol. Prog. 16 (2000) 254-257
31. Ziauddin J., and Sabatini D.M. Microarrays of cells expressing defined cDNAs. Nature 411 (2001) 107-110