Semi-quantitative expression and knockdown of a target gene in single-cell mouse embryonic stem cells by high performance microinjection

Biotechnology Letters

Volumn 29, Issue 3, 2007, Pages 341-350

  Matsuoka, Hideaki ^a,c   Shimoda, Soichiro ^a   Ozaki, Masakazu ^a   Mizukami, Hajime ^a   Shibusawa, Meiri ^a   Yamada, Yohei ^b,c   Saito, Mikako ^a,c  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b Chuo Precision Industrial Co Ltd   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Embryonic stem cell; Gene expression control; Knockdown; RNAi; Semi quantitative microinjection

Indexed keywords

CELLS; CHEMICAL ANALYSIS; CONCENTRATION (PROCESS); GENES; RNA;

EMBRYONIC STEM CELLS; GENE EXPRESSION CONTROL; SEMIQUANTITATIVE MICROINJECTION;

GENETIC ENGINEERING;

SMALL INTERFERING RNA;

ANIMAL; ARTICLE; CELL LINE; CULTURE TECHNIQUE; EMBRYONIC STEM CELL; GENE SILENCING; GENE TARGETING; METHODOLOGY; MICROINJECTION; MOUSE; MOUSE MUTANT; PHYSIOLOGY; PLASMID; ROBOTICS;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; EMBRYONIC STEM CELLS; GENE SILENCING; GENE TARGETING; MICE; MICE, KNOCKOUT; MICROINJECTIONS; PLASMIDS; RNA, SMALL INTERFERING; ROBOTICS;

EID: 33847257964     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9247-8     Document Type: Article

References (22)

1. Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell-Badge R (2003) Multipotent cell lineages in early mouse development depend on Sox-2 function. Genes Dev 17:126-140
2. Bavner A, Sanyal S, Gustafsson JK, Treuter E (2005) Transcriptional corepression by SHP: molecular mechanisms and physiological consequences. Trends Endocrinol Metab 16:478-488
3. Fujikura J, Yamato E, Yonemura S, Hosoda K, Masui S, Nakao K, Miyazaki J, Niwa H (2002) Differentiation of embryonic stem cells is induced by GATA factors. Genes Dev 16:784-789
4. Garcia AL, Ista LK, Petsev DN, O'Brien MJ, Bisong P, Mammoli AA, Brueck SRJ, Ló pez GP (2005) Electrokinetic molecular separation in nanoscale fluidic channels. Lab Chip 5:1271-1276
5. Gerecht-Nir S, Itskovitz-Eldor J (2004) Cell therapy using human embryonic stem cells. Transpl Immunol 12:203-209
6. Heng BC, Liu H, Cao T (2005) Transplanted human embryonic stem cells as biological 'catalysis' for tissue repair and regeneration. Med Hypotheses 64:1085-1088
7. Kim J-Y, Chu K, Kim H-J, Seong H-A, Park K-C, Sanyal S, Takeda J, Ha H, Shong M, Tsai M-J, Choi H-S (2004) Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/NeuroD. Mol Endocrinol 18:776-790
8. Lerou PH, Daley GQ (2005) Therapeutic potential of embryonic stem cells. Blood Rev 19:321-331
9. Matsuda T, Nakamura T, Nakao K, Arai T, Katsuki M, Heike T, Yokota T (1999) STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells. EMBO J 18:4261-4269
10. Matsuoka H, Komazaki T, Mukai Y, Shibusawa M, Akane H, Chaki A, Uetake N, Saito M (2005) High throughput easy microinjection with a single-cell manipulation supporting robot. J Biotechnol 116:185-194
11. Matsuoka H, Saito M (2006) High throughput microinjection technology toward single-cell bioelectrochemistry. Electrochemistry 74:12-18
12. Matsuoka H, Shimoda S, Miwa Y, Saito M (2006) Automatic positioning of a microinjector in mouse ES cells and rice protoplasts. Bioelectrochemistry 69:187-192
13. Nishigori H, Tomura H, Tonooka N, Kanamori M, Yamada S, Sho K, Inoue I, Kikuchi N, Onigata K, Kojima I, Kohama T, Yamagata K, Yang Q, Matsuzawa Y, Miki T, Seino S, Kim MY, Choi HS, Lee YK, Moore DD, Takeda J (2001) Mutations in the small heterodimer partner gene are associated with mild obesity in Japanese subjects. Proc Natl Acad Sci USA 98:2575-2580
14. Nishimoto M, Miyagi S, Yamagishi T, Sakaguchi T, Niwa H, Muramatsu M, Okuda A (2005) Oct-3/4 maintains the proliferative embryonic stem cell state via specific binding to a variant octamer sequence in the regulatory region of the UTF1 locus. Mol Cell Biol 25:5084-5094
15. Niwa H, Miyazaki J, Smith AG (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation, or selfrenewal of ES cells. Nat Genet 24:372-376
16. Park J, Jung S-H, Kim Y-H, Kim B, Lee S-K, Park J-O (2005) Design and fabrication of an integrated cell processor for single embryo cell manipulation. Lab Chip 5:91-96
17. Rettig JR, Folch A (2005) Large-scale single-cell trapping and imaging using microwell arrays. Anal Chem 77:5628-5634
18. Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D (1988) Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 336:688-690
19. Smith AG (1991) Culture and differentiation of embryonic stem cells. J Tissue Cult Methods 13:89-94
20. Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K (2004) Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference. Nucleic Acids Res 32:936-948
21. Wang K, Yue S, Wang L, Jin A, Gu C, Wang P, Wang H, Xu X, Wang Y, Niu H (2006) Nanofluidic channels fabrication and manipulation of DNA molecules. IEE Proc Nanobiotechnol 153:11-15
22. Yasukawa T, Glidle A, Cooper JM, Matsue T (2002) Electroanalysis of metabolic flux from single cells in picolitre-volume microsystems. Anal Chem 74:5001-5008