Estimation of luminal mucin content in rats by measurement of O-linked oligosaccharide chains and direct ELISA

Bioscience, Biotechnology and Biochemistry

Volumn 71, Issue 2, 2007, Pages 575-578

  Tanabe, Hiroki ^a   Ito, Hiroyuki ^a   Sugiyama, Kimio ^a   Kiriyama, Shuhachi ^b   Morita, Tatsuya ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

ELISA; Mucins; O linked oligosaccharide chains; Rat

Indexed keywords

BIOMARKERS; CORRELATION METHODS; GROWTH KINETICS; METABOLISM;

MUCINS; O-LINKED OLIGOSACCHARIDE CHAINS (OSC); OLIGOSACCHARIDE;

IMMUNOLOGY;

BIOLOGICAL MARKER; MUCIN; OLIGOSACCHARIDE; SIALIC ACID DERIVATIVE;

ANIMAL; ARTICLE; BODY WEIGHT; DIET; DRUG EFFECT; EATING; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOHISTOCHEMISTRY; MALE; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; WEIGHT GAIN; WISTAR RAT;

ANIMALS; BIOLOGICAL MARKERS; BODY WEIGHT; DIET; EATING; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOHISTOCHEMISTRY; MALE; MUCINS; OLIGOSACCHARIDES; RATS; RATS, WISTAR; SIALIC ACIDS; WEIGHT GAIN;

RATTUS;

EID: 33847239856     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60448     Document Type: Article

References (11)

1. Corfield, A. P., Myerscough, N., Longman, R., Sylvester, P., Arul, S., and Pignatelli, M., Mucins and mucosal protection in the gastrointestinal tract: new prospects for mucins in the pathology of gastrointestinal disease. Gut, 47, 589-594 (2000).
2. Satchithanandam, S., Vargofcak-Apker, M., Calvert, R. J., and Cassidy, M. M., Alteration of gastrointestinal mucin by fiber feeding in rats. J. Nutr., 120, 1179-1184 (1990).
3. Satchithanandam, S., Klurfeld, D. M., Calvert, R. J., and Cassidy, M. M., Effects of dietary fibers on gastrointestinal mucin in rats. Nutr. Res., 16, 1163-1177 (1996).
4. Tanabe, H., Sugiyama, K., Matsuda, T., Kiriyama, S., and Morita, T., Small intestinal mucins are secreted in proportion to the settling volume in water of dietary indigestible components in rats. J. Nutr., 135, 2431-2437 (2005).
5. Satchithanandam, S., Cassidy, M. M., Kharroubi, A. T., Calvert, R. J., Leeds, A. R., and Vahouny, G. V., Alterations in rat intestinal mucin patterns following luminal infusion of acetylsalicylic acid and prostaglandin derivatives. Dig. Dis. Sci., 35, 1518-1527 (1990).
6. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275 (1954).
7. Mantle, M., and Allen, A., A colorimetric assay for glycoprotein based on the periodic acid/Schiff's stain. Biochem. Soc. Trans., 6, 607-609 (1978).
8. Morita, T., Tanabe, H., Takahashi, K., and Sugiyama, K., Ingestion of resistant starch protects endotoxin influx from the intestinal tract and reduces D-galactosamine-induced liver injury in rats. J. Gastroenterol. Hepatol., 19, 303-313 (2004).
9. Vaitukaitis, J., Robbins, J. B., Nieschlag, E., and Ross, G. T., A method for producing specific antisera with small doses of immunogen. J. Clin. Endocrinol., 33, 988-991 (1971).
10. Tanabe, H., Ito, H., Sugiyama, K., Kiriyama, S., and Morita, T., Dietary indigestible components exert different regional effects on luminal mucin secretion through their bulk-forming property and fermentability. Biosci. Biotechnol. Biochem., 70, 1188-1194 (2006).
11. Van Klinken, B. J. W., Einerhand, A. W. C., Buller, H. A., and Dekker, J., Strategic biochemical analysis of mucins. Anal. Biochem., 265, 103-116 (1998).