Internalization and distribution of three α1-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation

Acta Pharmacologica Sinica

Volumn 28, Issue 3, 2007, Pages 359-366

  Wang, Shu Yi ^a   Song, Yao ^a   Xu, Ming ^a   He, Qi Hua ^b   Han, Qi De ^a   Zhang, You Yi ^a  

^a PEKING UNIVERSITY THIRD HOSPITAL   (China)

^b PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

3H prazosin binding assay; Adrenoceptor; Agonist stimulation; Confocal image; Enzyme linked immunosorbent assay; Internalization; Subcellular distribution; Subtype

Indexed keywords

ALPHA 1 ADRENERGIC RECEPTOR; ALPHA 1A ADRENERGIC RECEPTOR; ALPHA 1B ADRENERGIC RECEPTOR; ALPHA 1D ADRENERGIC RECEPTOR; CYTOPLASMIC RECEPTOR; PHENYLEPHRINE; PRAZOSIN; RECEPTOR SUBTYPE; ALPHA ADRENERGIC RECEPTOR BLOCKING AGENT; ALPHA ADRENERGIC RECEPTOR STIMULATING AGENT; MITOGEN ACTIVATED PROTEIN KINASE;

ARTICLE; BINDING ASSAY; CELL COMPOSITION; CELL STRAIN HEK293; CELL SURFACE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBRYO; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; INTERNALIZATION; PROTEIN LOCALIZATION; WHOLE CELL; BIOSYNTHESIS; CELL LINE; DRUG POTENTIATION; GENETIC TRANSFECTION; GENETICS; METABOLISM;

ADRENERGIC ALPHA-AGONISTS; ADRENERGIC ALPHA-ANTAGONISTS; CELL LINE; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; HUMANS; PHENYLEPHRINE; PRAZOSIN; RECEPTORS, ADRENERGIC, ALPHA-1; TRANSFECTION;

EID: 33847197960     PISSN: 16714083     EISSN: 17457254     Source Type: Journal    
DOI: 10.1111/j.1745-7254.2007.00509.x     Document Type: Article

References (24)

1. Milligan G. Constitutive activity and inverse agonists of G protein-coupled receptors: a current perspective. Mol Pharmacol 2003; 64: 1271-6.
2. Graham RM, Perez DM, Hwa J, Piascik MT. α1-Adrenergic receptor subtypes. Molecular structure, function and signaling. Circ Res 1996; 78: 737-49.
3. Zhong H, Minneman KP. Differential activation of mitogen-activated protein kinase pathways in PC12 cells by closely related alpha1-adrenergic receptor subtypes. J Neurochem 1999; 72: 2388-96.
4. Schwinn DA, Johnston GI, Page SO, Mosley MJ, Wilson KH, Worman NP, et al. Cloning and pharmacological characterization of human α1-adrenergic receptors: sequence corrections and direct comparison with other species homologues. J Pharmacol Exp Ther 1995; 272: 134-42.
5. Rokosh DG, Stewart AF, Chang KC, Bailey BA, Karliner JS, Camacho SA, et al. α1-Adrenergic receptor subtype mRNAs are differentially regulated by α1-adrenergic and other hypertrophic stimuli in cardiac myocytes in culture and in vivo. J Biol Chem 1996; 271: 5839-43.
6. Fonseca MI, Button DC, Brown RD. Agonist regulation of α1B-adrenergic receptor subcellular distribution and function. J Biol Chem 1995; 270: 8902-9.
7. Hirasawa A, Sugawara T, Awaji T, Tsumaya K, Ito H, Tsujimoto G. Subtype-specific differences in subcellular localization of α1- adrenoceptors (ARs): chloroethylclonidine preferentially alkylates the accessible surface α1-ARs irrespective of subtype. Mol Pharmacol 1997; 52: 764-70.
8. Hrometz SL, Edelmann SE, McCune DF, Olges JR, Hadley RW, Perez DM, et al. Expression of multiple alpha1-adrenergic receptors on vascular smooth muscle: correlation with the regulation of contraction. J Pharmacol Exp Ther 1999; 290: 452-63.
9. Sugawara T, Hirasawa A, Hashimoto K, Tsujimoto G. Differences in the subcellular localization of alpha1-adrenoceptor subtypes can affect the subtype selectivity of drugs in a study with the fluorescent ligand BODIPY FL-prazosin. Life Sci 2002; 70: 2113- 24.
10. Diviani D, Lattion AL, Larbi N, Kunapuli P, Pronin A, Benovic JL, et al. Effect of different G protein-coupled receptor kinases on phosphorylation and desensitization of the alpha1B-adrenergic receptor. J Biol Chem 1996; 271: 5049-58.
11. Diviani D, Lattion AL, Cotecchia S. Characterization of the phosphorylation sites involved in G protein-coupled receptor kinase- and protein kinase C-mediated desensitization of the alpha1B-adrenergic receptor. J Biol Chem 1997; 272: 28712-9.
12. Chalothorn D, McCune DF, Edelmann SE, Garcia-Cazarin ML, Tsujimoto G, Piascik MT. Differences in the cellular localization and agonist-mediated internalization properties of the α1-adrenoceptor subtypes. Mol Pharmacol 2002; 61: 1008-16.
13. Hague C, Uberti MA, Chen Z, Hall RA, Minneman KP. Cell surface expression of α1D-adrenergic receptors is controlled by heterodimerization with α1B-adrenergic receptors. J Biol Chem 2004; 279: 15 541-9.
14. Vazquez-Prado J, Medina LC, Romero-Avila MT, Gonzalez-Espinosa C, Garcia-Sainz JA. Norepinephrine and phorbol ester-induced phosphorylation of α(1a)-adrenergic receptor. J Biol Chem 2000; 275: 6553-9.
15. Yang M, Ruan J, Voller M, Schalken J, Michel MC. Differential regulation of human α1-adrenoceptor subtypes. Naunyn-Schmiedeberg's Arch Pharmacol 1999; 359: 439-46.
16. Garcia-Sainz JA, Vazquez-Cuevas FG, Romero-Avila MT. Phosphorylation and desensitization of α1D-adrenergic receptors. Biochem J 2001; 353: 603-10.
17. Ferguson SS. Evolving concepts in G protein-coupled receptor endocytosis: the role in receptor desensitization and signaling. Pharmacol Rev 2001; 53: 1-24.
18. Wang S, Song Y, Xu M, Hao T, Han Q, Zhang Y. Redistribution of three α1-adrenergic receptor subtypes in the stably transfected HEK 293A cells upon agonist stimulation. Acta Physiol Sin 2004; 57: 480-5.
19. Eason MG, Liggett SB. Subtype-selective desensitization of alpha 2-adrenergic receptors. Different mechanisms control short and long term agonist-promoted desensitization of alpha 2C10, alpha 2C4, and alpha 2C2. J Biol Chem 1992; 267: 25 473-9.
20. Minneman KP, Esbenshade TA. Alpha 1-adrenergic receptor subtypes. Annu Rev Pharmacol Toxicol 1994; 34: 117-33.
21. Awaji T, Hirasawa A, Kataoka M, Shinoura H, Nakayama Y, Sugawara T, et al. Real-time optical monitoring of ligand-mediated internalization of α1b-adrenoceptor with green fluorescent protein. Mol Endocrinol 1998; 12: 1099-111.
22. Garcia-Sainz JA, Vazquez-Prado J, Villalobos-Molina R. α1-adrenoceptors: subtypes, signaling and roles in health and disease. Arch Med Res 1999; 31: 449-58.
23. McGrath JC, Mackenzie JF, Daly CJ. Pharmacological implications of cellular localization of alpha1-adrenoceptors in native smooth muscle cells. J Auton Pharmacol 1999; 19: 303-10.
24. Bunemann M, Lee KB, Pals-Rylaarsdam R, Roseberry AG, Hosey MM. Desensitization of G-protein-coupled receptors in the cardiovascular system. Annu Rev Physiol 1999; 61: 169-92.