Chromatographic separation and quantification of major human milk proteins

Journal of Liquid Chromatography and Related Technologies

Volumn 30, Issue 4, 2007, Pages 499-507

  Ferreira, Isabel M P L V O ^a  

^a UNIVERSITY OF PORTO   (Portugal)

Author keywords

Lactalbumin; Caseins; HPLC; Human milk; Proteins

Indexed keywords

COPOLYMERS; CORRELATION METHODS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LINEARIZATION; POLYSTYRENES; ULTRAVIOLET RADIATION;

HPLC; HUMAN CASEIN; POLYSTYRENE DIVINYLBENZENE COPOLYMER; REVERSE PHASE COLUMN CHROMPACK P 300 RP;

CASEIN;

ALPHA LACTALBUMIN; CASEIN; COPOLYMER; DIVINYLBENZENE; MILK PROTEIN; POLYSTYRENE; WATER;

ARTICLE; CALIBRATION; CHROMATOGRAPHY; CONTROLLED STUDY; CORRELATION ANALYSIS; CORRELATION COEFFICIENT; DILUTION; FEMALE; FREEZE DRYING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; QUANTITATIVE ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; STANDARDIZATION; ULTRAVIOLET RADIATION; VALIDITY;

EID: 33847118146     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070601093796     Document Type: Article

References (26)

1. Cavaletto, M.; Cantisani, A.M.; Napolitano, L.; Giuffrida, M.G.; Calderone, V.; Fabris, C.; Bertino, E.; Prandi, M.G.; Conti, A. Comparative study of casein content in human colostrum and milk. Milchwissenschaft 1994, 49, 303-305.
2. Kroening, T.A.; Mukerji, P.; Hards, R.G. Analysis of beta-casein and its phosphoforms in human milk. Nutr. Res. 1998, 18, 1175-1186.
3. Forsum, E. Nutritional evaluation of whey protein concentrates and their fractions. J. Dairy Sci. 1974, 57, 665-670.
4. Finlay, J.; Brew, K. The complete amino acid sequence of human α-lactalbumin. Eur. J. Biochem. 1972, 27, 65-72.
5. Brew, K.; Castellino, F.; Vanoman, D.; Hill, R. The complete amino acid sequence of bovine a-lactalbumin. J. Biol. Chem. 1970, 245, 4570-4582.
6. Montagne, P.M.; Cuillière, M.L.; Mole, C.M.; Béné, M.C.; Faure, G.C. Dynamics of the main immunologically and nutritionally available proteins of human milk during lactation. J. Food Comp. Anal. 2000, 13, 127-137.
7. Kunz, C.; Lönnerdal, B. Re-evaluation of the whey protein/casein ratio of human milk. Acta Paediatr. 1992, 81, 107-112.
8. Kunz, C.; Lonnerdal, B. Human-milk proteins: analysis of casein and casein subunits by anion-exchange chromatography, gel electrophoresis, and specific staining methods. Am. J. Clin. Nut. 1990, 51, 37-46.
9. Dev, B.C.; Sood, S.M.; Dewind, S.; Slattery, C.W. Characterization of human kappa-casein purified by FPLC. Prep. Biochem. 1993, 23, 389-407.
10. Sood, S.M.; Slattery, C.W. Suspension of the calcium-sensitive human β-caseins by human k-Casein. J. Dairy Sci. 2002, 85, 1353-1356.
11. Sood, S.M.; Erickson, G.; Slattery, C.W. k-Casein Interactions in the suspension of the two major calcium-sensitive human β-caseins. J. Dairy Sci. 2003, 86, 2269-2275.
12. Lonnerdal, B.; Forsum, E. Casein content of human milk. Am. J. Clin. Nutr. 1985, 41, 113-120.
13. Chtourou, A.; Brignon, G.; Ribadeau-Dumas, B. Quantification of β-casein in human milk. J. Dairy Res. 1985, 52, 239-247.
14. Greenberg, R.; Groves, M.L.; Dower, H. Human β-casein. J. Biol. Chem. 1984, 259, 5132-5138.
15. Bernard, H.; Negroni, L.; Chatel, J.M.; Clement, G.; Adel-Patient, K.; Peltre, G.; Creminon, C.; Wal, J.M. Molecular basis of IgE cross-reactivity between human β-casein and bovine β-casein, a major allergen of milk. Molec. Immunol. 2000, 37, 161-167.
16. Mellander, O.; Folsch, G. Enzyme resistance and metal binding of phosphorylated peptides. In Protein and Amino Acid Functions; Bigwood, E., Ed.; Pergamon Press: Oxford, 1972, 569-579.
17. Nagasawa, T.; Kiyosawa, J.; Takase, M. Lactoferrin and serum albumin of human casein in colostrum and milk. J. Dairy Sci. 1974, 57, 1159-1163.
18. Lonnerdal, B. Nutritional and physiologic significance of human milk proteins. J. Clin. Nutr. 2003, 77, 1537S-1543S.
19. Montagne, P.M.; Cuillière, M.L.; Molé, C.M.; Béné, M.C.; Faure, G.C. Dynamics of the main immunologically and nutritionally available proteins of human milk during lactation. J. Food Comp. Anal. 2000, 13, 127-137.
20. Kunz, C.; Lonnerdal, B. Human milk proteins: separation of whey proteins and their analysis by polyacrylamide gel electrophoresis, fast protein liquid chromatography (FPLC) gel filtration and anion-exchange chromatography. Am. J. Clin. Nutr. 1989, 49, 464-470.
21. Jackson, J.G.; Janszen, D.B.; Lonnerdal, B.; Lien, E.L.; Pramuk, K.P.; Kuhlman, C.F. A multinational study of α-lactalbumin concentrations in human milk. J. Nutr. Biochem. 2004, 15, 517-521.
22. Chowanadisai, W.; Kelleher, S.L.; Nemeth, J.F.; Yachetti, S.; Kuhlman, C.F.; Jackson, J.G.; Davis, A.M.; Lien, E.L.; Lonnerdal, B. Detection of a single nucleotide polymorphism in the human α-lactalbumin gene: implications for human milk proteins. J. Nutr. Biochem. 2005, 16, 272-278.
23. Dev, B.C.; Sood, S.M.; Dewind, S.; Slattery, C.W. Characterization of human κ-casein purified by FPLC. Prep. Biochem. 1993, 23, 389-407.
24. Cuillière, M.L.; Trégoat, V.; Béné, M.C.; Faure, G.; Montagne, P. Changes in the κ-casein and β-casein concentrations in human milk during lactation. J. Clin. Lab. Anal. 1999, 13, 213-218.
25. Bernard, H.; Negroni, L.; Chatel, J.M.; Clement, G.; Adel-Patient, K.; Peltre, G.; Creminon, C.; Wal, J.M. Molecular basis of IgE cross-reactivity between human β-casein and bovine β-casein, a major allergen of milk. Mol. Immunol. 2000, 37, 161-167.
26. Ferreira, I.M.P.L.V.O.; Mendes, E.; Ferreira, M.A. HPLC/UV analysis of proteins in dairy products using a hydrophobic interaction chromatographic column. Anal. Sci. 2001, 17, 499-501.