Simple and rapid detection of the porcine reproductive and respiratory syndrome virus from pig whole blood using filter paper

Journal of Virological Methods

Volumn 141, Issue 1, 2007, Pages 102-106

  Inoue, Ryo ^a,b   Tsukahara, Takamitsu ^a,b   Sunaba, Chinatsu ^a   Itoh, Mitsugi ^c   Ushida, Kazunari ^a  

^a KYOTO PREFECTURAL UNIVERSITY   (Japan)

^b Ujitawara cho   (Japan)

^c Akabane Animal Clinic   (Japan)

Author keywords

Flinders Technology Associates filter paper; Pig; PRRSV; Real time RT PCR

Indexed keywords

RNA VACCINE;

ADOLESCENT; ANIMAL CELL; ANIMAL TISSUE; ARTERIVIRUS; ARTICLE; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; SWINE; VIRUS DETECTION;

ANIMALS; BASE SEQUENCE; FILTRATION; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PAPER; POLYMERASE CHAIN REACTION; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; RNA, VIRAL; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUS SCROFA; TIME FACTORS;

PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE;

EID: 33847110339     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.11.030     Document Type: Article

References (18)

1. Albina E., Leforban Y., Baron T., Plana Duran J.P., and Vannier P. An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus. Ann. Rech. Vet. 23 (1992) 167-176
2. Albina E. Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet. Microbiol. 55 (1997) 309-316
3. Christopher-Hennings J., Nelson E.A., Nelson J.K., and Benfield D.A. Effects of a modified-live virus vaccine against porcine reproductive and respiratory syndrome in boars. Am. J. Vet. Res. 58 (1997) 40-45
4. Christopher-Hennings J., Holler L.D., Benfield D.A., and Nelson E.A. Detection and duration of porcine reproductive and respiratory syndrome virus in semen, serum, peripheral blood mononuclear cells, and tissues from Yorkshire, Hampshire, and Landrace boars. J. Vet. Diagn. Invest. 13 (2001) 133-142
5. Chung W.B., Chan W.H., Chaung H.C., Lien Y., Wu C.C., and Huang Y.L. Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally infected and challenged pigs. J. Virol. Methods 124 (2005) 11-19
6. Egli C., Thur B., Liu L., and Hofman M.A. Quantitative Taqman RT-PCR for the detection and differentiation of European and North American strains of porcine reproductive and respiratory syndrome virus. J. Virol. Methods 98 (2001) 63-75
7. Gilbert S.A., Larochelle R., Magar R., Cho H.J., and Deregt D. Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay. J. Clin. Microbiol. 35 (1997) 264-267
8. Higgins J.A., Ibrahim M.S., Knauert F.K., Ludwig G.V., Kijek T.M., Ezzell J.W., Courtney B.C., and Henchal E.A. Sensitive and rapid identification of biological threat agents. Ann. N.Y. Acad. Sci. 894 (1999) 130-148
9. Lager K.M., Mengeling W.L., and Brockmeier S.L. Duration of homologous porcine reproductive and respiratory syndrome virus immunity in pregnant swine. Vet. Microbiol. 58 (1997) 127-133
10. Mavromatis I., Kritas S.K., Alexopoulos C., Tsinas A., and Kyriakis S.C. Field evaluation of a live vaccine against porcine reproductive and respiratory syndrome in fattening pigs. J. Vet. Med. B 46 (1999) 603-612
11. Moscoso H., Raybon E.O., Thayer S.G., and Hofacre C.L. Molecular detection and serotyping of infectious bronchitis virus from FTA filter paper. Avian. Dis. 49 (2005) 24-29
12. Meulenberg J.J., Hulst M.M., de Meijer E.J., Moonen P.L., den Besten A., de Kluyver E.P., Wensvoort G., and Moormann R.J. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. J. Virol. 19 (1993) 62-72
13. Oleksiewicz M.B., Botner A., Madsen K.G., and Storgaard T. Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes. Vet. Microbiol. 64 (1998) 7-22
14. Pejsak Z., Stadejek T., and Markowska-Daniel I. Clinical signs and economic losses caused by porcine reproductive and respiratory syndrome virus in a large breeding farm. Vet. Microbiol. 55 (1997) 3317-3322
15. Perozo F., Villegas P., Estevez C., Alvarado I., and Purvis L.B. Use of FTA filter paper for the molecular detection of Newcastle disease virus. Avian. Pathol. 35 (2006) 93-98
16. ®). Biotechnol. Appl. Biochem. 31 (2000) 219-224
17. Suarez P., Zardoya R., Prieto C., Solana A., Tabares E., Bautista J.M., and Castro J.M. Direct detection of the porcine reproductive and respiratory syndrome (PRRS) virus by reverse polymerase chain reaction (RT-PCR). Arch. Virol. 135 (1994) 89-99
18. van Woensel P.A., Liefkens K., and Demaret S. European serotype PRRSV vaccine protects against European serotype challenge whereas an American serotype vaccine does not. Adv. Exp. Med. Biol. 440 (1998) 713-718