Determination of free l-carnitine in human seminal plasma by high performance liquid chromatography with pre-column ultraviolet derivatization and its clinical application in male infertility

Clinica Chimica Acta

Volumn 378, Issue 1-2, 2007, Pages 159-163

  Li, Ke ^a   Li, Wei ^a   Huang, Yufeng ^a  

^a JINLING HOSPITAL   (China)

Author keywords

Free l carnitine; High performance liquid chromatography; Infertility; Seminal plasma

Indexed keywords

ACETONITRILE; BIOCHEMICAL MARKER; CARNITINE; CARNITINE DERIVATIVE; CITRIC ACID; ESTER; METHANOL; OXYGEN; PROTEIN; SILICON; TRIETHANOLAMINE;

AMINO ACID ANALYSIS; ARTICLE; ASTHENOSPERMIA; BIOASSAY; CONTROLLED STUDY; DERIVATIZATION; ELUTION; FERTILITY; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; INFERTILITY; LIGHT ABSORPTION; LINEAR SYSTEM; MALE; OLIGOASTHENOTERATOZOOSPERMIA; OLIGOSPERMIA; PRIORITY JOURNAL; SAMPLING; SEMINAL PLASMA; SPERM; SPERMATOZOON ABNORMALITY; ULTRAVIOLET RADIATION;

ADULT; CARNITINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; INFERTILITY, MALE; MALE; REPRODUCIBILITY OF RESULTS; SEMEN;

EID: 33846915979     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2006.11.008     Document Type: Article

References (23)

1. Bianchetti A., Rozzini R., and Trabucchi M. Effects of acetyl-l-carnitine in Alzheimer's disease patients unresponsive to acetylcholinesterase inhibitors. Curr Med Res Opin 19 4 (2003) 350-353
2. Werbach M.R. Nutritional strategies for treating chronic fatigue syndrome. Altern Med Rev 5 2 (2000) 93-108
3. Bellinghieri G., Santoro D., Calvani M., Mallamace A., and Savica V. Carnitine and hemodialysis. Am J Kidney Dis 41 3 Suppl 1 (2003) S116-S122
4. Beshlawy A., Ragab L., Fattah A.A., et al. Improvement of cardiac function in thalassemia major treated with l-carnitine. Acta Haematol 111 3 (2004) 143-148
5. Brass E.P., and Hiatt W.R. The role of carnitine and carnitine supplementation during exercise in man and in individuals with special needs. J Am Coll Nutr 17 3 (1998) 207-215
6. Cavallini G., Modenini F., Vitali G., and Koverech A. Acetyl-l-carnitine plus propionyl-l-carnitine improve efficacy of sildenafil in treatment of erectile dysfunction after bilateral nerve-sparing radical retropubic prostatectomy. Urology 66 5 (2005) 1080-1085
7. Lenzi A., Lombardo F., Sgro P., et al. Use of carnitine therapy in selected cases of male factor infertility: a double-blind crossover trial. Fertil Steril 79 2 (2003) 292-300
8. Lenzi A., Sgro P., Salacone P., et al. A placebo-controlled double-blind randomized trial of the use of combined l-carnitine and l-acetyl-carnitine treatment in men with asthenozoospermia. Fertil Steril 81 6 (2004) 1578-1584
9. Balercia G., Regoli F., Armeni T., Koverech A., Mantero F., and Boscaro M. Placebo-controlled double-blind randomized trial on the use of l-carnitine, l-acetylcarnitine, or combined l-carnitine and l-acetylcarnitine in men with idiopathic asthenozoospermia. Fertil Steril 84 3 (2005) 662-671
10. McEntyre C.J., Lever M., and Storer M.K. A high performance liquid chromatographic method for the measurement of total carnitine in human plasma and urine. Clin Chim Acta 344 1-2 (2004) 123-130
11. Minkler P.E., Kerner J., North K.N., and Hoppel C.L. Quantitation of long-chain acylcarnitines by HPLC/fluorescence detection: application to plasma and tissue specimens from patients with carnitine palmitoyltransferase-II deficiency. Clin Chim Acta 352 1-2 (2005) 81-92
12. Vernez L., Wenk M., and Krahenbuhl S. Determination of carnitine and acylcarnitines in plasma by high-performance liquid chromatography/electrospray ionization ion trap tandem mass spectrometry. Rapid Commun Mass Spectrom 18 11 (2004) 1233-1238
13. Hardy D.T., Preece M.A., and Green A. Determination of plasma free carnitine by electrospray tandem mass spectrometry. Ann Clin Biochem 38 Pt 6 (2001) 665-670
14. Rowe P.J., Comhaire F.H., Hargreave T.B., and Mahmond A.M.A. WHO manual for the standardized investigation, diagnosis and management of the infertile male (2000), Cambridge University Press, Cambridge 17
15. Agarwal A., and Said T.M. Carnitine and male infertility. Reprod Biomed Online 8 4 (2004) 376-384
16. Ng C.M., Blackman M.R., Wang C., and Swerdliff R.S. The role of carnitine in the male reproductive system. Ann NY Acad Sci 1033 (2004) 177-188
17. Juliet P.A., Joyee A.G., Jayaraman G., Mohankumar M.N., and Panneerselvam C. Effect of l-carnitine on nucleic acid status of aged rat brain. Exp Neurol 191 1 (2005) 33-40
18. Dokmeci D. Oxidative stress, male infertility and the role of carnitines. Folia Med (Plovdia) 47 1 (2005) 26-30
19. Vicari E., La Vignera S., and Calogero A.E. Antioxidant treatment with carnitines is effective in infertile patients with prostatovesiculoepididymitis and elevated seminal leukocyte concentrations after treatment with nonsteroidal anti-inflammatory compounds. Fertil Steril 78 6 (2002) 1203-1208
20. Khademi A., Alleyassin A., Safdarian L., Hamed E.A., Rabiee E., and Haghaninezhad H. The effects of l-carnitine on sperm parameters in smoker and non-smoker patients with idiopathic sperm abnormalities. J Assist Reprod Genet 22 11-12 (2005) 395-399
21. Garolla A., Maiorini M., Roverato A., Roveri A., Ursini F., and Foresta C. Oral carnitine supplementation increases sperm motility in asthenozoospermic men with normal sperm phospholipid hydroperoxide glutathione peroxidase levels. Fertil Steril 84 3 (2005) 662-671
22. Zopfgen A., Priem F., Sudhoff F., et al. Relationship between semen quality and the seminal plasma components carnitine, alpha-glucosidase, fructose, citrate and granulocyte elastase in infertile men compared with a normal population. Hum Reprod 15 4 (2000) 840-845
23. Gurbuz B., Yalti S., Ficicioglu C., and Zehir K. Relationship between semen quality and seminal plasma total carnitine in infertile men. J Obstet Gynaecol 23 6 (2003) 653-656