Presence of milk clotting proteinases in Cynara scolymus L. cv. Green Globe (Asteraceae)

Acta Horticulturae

Volumn 501, Issue , 1999, Pages 249-257

  Llorente, B ^a   Brutti, C ^a   Cimino, C ^b   Vairo Cavalli, S ^b   Natalucci, C ^b   Caffini, N ^b  

^a DEPARTAMENTO DE CIENCIAS BÁSICAS   (Argentina)

^b FACULTAD DE CIENCIAS EXACTAS   (Argentina)

Author keywords

Artichoke; Aspartic proteinases; Asteraceae; Cynara scolymus cv. Green Globe; Milk clotting enzymes

Indexed keywords

EID: 33846880653     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/actahortic.1999.501.40     Document Type: Conference Paper

References (18)

1. Asakura T., Watanabe H., Abe K., and Arai S., 1995. Rice aspartic proteinase, oryzasin, expressed during seed ripening and germination, has a gene organization distinct from those of animal and microbial aspartic proteinases. Eur. J. Biochem. 232:77-83.
2. Belozersky M. A., Sarbakanova S. T. and Dunaevsky Y. E., 1989. Aspartic proteinase from wheat seeds: isolation, properties and action on gliadin. Planta. 177:321-326.
3. Bradford M. M., 1976. A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254.
4. Cordeiro M. C., Jakob E., Puhan Z., Pais M. S. and Brodelius P. E., 1992. Milk clotting and proteolytic activities of purified cynarases from Cynara cardunculus - a comparison to chymosin. Milchwissenschaft 47:681-687.
5. Cordeiro M. C., Pais M. S. and Brodelius P. E., 1994b. Tissue-specific expression of multiple forms of cyprosin (aspartic proteinase) in flowers of Cynara candunculus. Physiol. Plant. 92:645-653.
6. Cordeiro M. C., Xue Z. T., Pietrzak M., Pais M. S. and Brodelius P. E., 1994a. Isolation and characterization of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin. Plant Mol. Biol. 24:733-741.
7. Dalgleish D. G., 1992. The enzymatic coagulation of milk, in Advanced Dairy Chemistry-1: Proteins (P. F. Fox, ed.). Elsevier Applied Science Publishers, London and New York. p. 581.
8. Heimgartner U., Pietrzak M., Geertsen R., Brodelius P., Da Silva Figuereido A. C. and Pais M. S. S., 1990. Purification and partial characterization of milk clotting proteases from flower of Cynara cardunculus. Phytochemistry 29:1405-1410.
9. Llorente B. E., Brutti C. B., Natalucci C. L. and Caffini, N. O., 1997. Partial characterization of a milk clotting proteinase isolated from artichoke (Cynara scolymus L., Asteraceae). Acta Farm. Bonaerense 16:37-42.
10. López L. M. I., 1995. Aislamiento, purificación y caracterización de las proteasas presentes en el látex de frutos de Madura pomífera (Raf.) Schneid. (Moraceae). Ph. D. Thesis. Fac. Ciencias Exactas, Univ. Nac. La Plata, Argentina.
11. Padmanabhan S., Chitre A., and Shastri N., 1993. Milk clotting protease isolated from Dieffenbachia maculata. Die Nahrung 37:99-101.
12. Priolo N. S., López L. M. I., Arribére M. C., Natalucci C. L., and Caffini N. O., 1991. New purified plant proteases for the food industry. Acta Alimentaria 20:189-196.
13. Ramalho-Santos M., Verissimo P, Faro C, and Pires E., 1996. Action on bovine aSlcasein of cardosins A and B, aspartic proteinases from the flower of the cardoon Cynara cardunculus L., Biochim. Biophys. Acta 1297:83-89.
14. Runeberg-Roos P., Kervinen J., Kovaleva V., Raikhe N. V. and Gal S., 1994. The aspartic proteinase of barley is a vacuolar enzyme that processes probarley lectin in vitro. Plant Physiol. 105:321-329.
15. Sarath G., De La Motte R. S., and Wagner F. W., 1989. Protease assay methods, in Proteolytic Enzymes (R. J. Beynon & J. S. Bond, eds.). IRL Press, Oxford, p. 27.
16. Verissimo P., Esteves C., Faro C., and Pires E., 1995. The vegetable rennet of Cynara cardunculus L. contains two proteinases with chymosin and pepsin-like specificities, Biotech. Lett. 17:621-626.
17. Verissimo P., Faro C., Moir A., Lin Y., Tang J., and Pires E., 1996. Purification, characterization and partial amino acid sequencing of two new aspartic proteinases from fresh flowers of Cynara cardunculus L. Eur. J. Biochem. 235:762-768.
18. Westergaar J. L., Hackbarth C., Treuhaft M. W., and Roberts R. C., 1980. Detection of proteinases in electrophoretograms of complex mixtures. J. Immunol. Meth. 34:167-175.