Low-affinity uptake of the fluorescent organic cation 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (4-Di-1-ASP) in BeWo cells

Biochemical Pharmacology

Volumn 73, Issue 6, 2007, Pages 891-900

  Rytting, Erik ^a   Bryan, Jordan ^b   Southard, Marylee ^c   Audus, Kenneth L ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b Oklahoma State University   (United States)

^c University of Kansas   (United States)

Author keywords

BeWo cells; Drug transport; OCT1; OCT2; Organic cation transporters; Placenta

Indexed keywords

1 METHYL 4 PHENYLPYRIDINIUM; 4 [4 (DIMETHYLAMINO)STYRYL] N METHYLPYRIDINIUM IODIDE; AGMATINE; AMILORIDE; ARGININE; CARNITINE; CATION; CHOLINE; COMPLEMENTARY DNA; CYTIDINE; FATTY ACID; GRAMICIDIN A; GUANOSINE; LEUCINE; MANNITOL; MEGLUMINE; METHAMPHETAMINE; ORGANIC CATION TRANSPORTER 1; ORGANIC CATION TRANSPORTER 2; RNA; SODIUM CHLORIDE; SPERMIDINE; TETRYLAMMONIUM CHLORIDE; THIONEINE; UNCLASSIFIED DRUG; VALINOMYCIN; VERAPAMIL;

ARTICLE; BASOLATERAL MEMBRANE; CATION TRANSPORT; CELL CULTURE; CHORIOCARCINOMA; CONTROLLED STUDY; DRUG TRANSPORT; DRUG UPTAKE; FETOPLACENTAL UNIT; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; NUCLEOTIDE SEQUENCE; PLACENTA; PRIORITY JOURNAL; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TROPHOBLAST;

BIOLOGICAL TRANSPORT; CELLS, CULTURED; FEMALE; HUMANS; MEMBRANE POTENTIALS; METHYLAMINES; OCTAMER TRANSCRIPTION FACTOR-1; ORGANIC CATION TRANSPORT PROTEINS; PYRIDINIUM COMPOUNDS; RNA, MESSENGER; TROPHOBLASTS;

EID: 33846875251     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bcp.2006.11.020     Document Type: Article

References (32)

1. Mason J.N., Farmer H., Tomlinson I.D., Schwartz J.W., Savchenko V., DeFelice L.J., et al. Novel fluorescence-based approaches for the study of biogenic amine transporter localization, activity, and regulation. J Neurosci Methods 143 (2005) 3-25
2. Ciarimboli G., Struwe K., Arndt P., Gorboulev V., Koepsell H., Schlatter E., et al. Regulation of the human organic cation transporter hOCT1. J Cell Physiol 201 (2004) 420-428
3. Cetinkaya I., Ciarimboli G., Yalcinkaya G., Mehrens T., Velic A., Hirsch J.R., et al. Regulation of human organic cation transporter hOCT2 by PKA, PI3K, and calmodulin-dependent kinases. Am J Physiol Renal Physiol 284 (2003) F293-F302
4. Ganapathy V., Prasad P.D., Ganapathy M.E., and Leibach F.H. Placental transporters relevant to drug distribution across the maternal-fetal interface. J Pharmacol Exp Ther 294 (2000) 413-420
5. Gorboulev V., Ulzheimer J.C., Akhoundova A., Ulzheimer-Teuber I., Karbach U., Quester S., et al. Cloning and characterization of two human polyspecific organic cation transporters. DNA Cell Biol 16 (1997) 871-881
6. Wessler I., Roth E., Deutsch C., Brockerhoff P., Bittinger F., Kirkpatrick C.J., et al. Release of non-neuronal acetylcholine from the isolated human placenta is mediated by organic cation transporters. Br J Pharmacol 134 (2001) 951-956
7. Koepsell H., and Endou H. The SLC22 drug transporter family. Pflugers Arch 447 (2004) 666-676
8. Koepsell H., Schmitt B.M., and Gorboulev V. Organic cation transporters. Rev Physiol Biochem Pharmacol 150 (2003) 36-90
9. Urakami Y., Akazawa M., Saito H., Okuda M., and Inui K. cDNA cloning, functional characterization, and tissue distribution of an alternatively spliced variant of organic cation transporter hOCT2 predominantly expressed in the human kidney. J Am Soc Nephrol 13 (2002) 1703-1710
10. You G. The role of organic ion transporters in drug disposition: an update. Curr Drug Metab 5 (2004) 55-62
11. Grundemann D., Liebich G., Kiefer N., Koster S., and Schomig E. Selective substrates for non-neuronal monoamine transporters. Mol Pharmacol 56 (1999) 1-10
12. Alcorn J., Lu X., Moscow J.A., and McNamara P.J. Transporter gene expression in lactating and nonlactating human mammary epithelial cells using real-time reverse transcription-polymerase chain reaction. J Pharmacol Exp Ther 303 (2002) 487-496
13. Sata R., Ohtani H., Tsujimoto M., Murakami H., Koyabu N., Nakamura T., et al. Functional analysis of organic cation transporter 3 expressed in human placenta. J Pharmacol Exp Ther 315 (2005) 888-895
14. Ming X., Bourdet D., and Thakker D. Gene expression profile of human organic cation transporters along the gastrointestinal tract and in Caco-2 cells. AAPS J 7 S2 (2005) Abstract R6268
15. Rytting E., and Audus K.L. Novel organic cation transporter 2-mediated carnitine uptake in placental choriocarcinoma (BeWo) cells. J Pharmacol Exp Ther 312 (2005) 192-198
16. Said H.M., Ortiz A., and Vaziri N.D. Mechanism and regulation of Vitamin B-6 uptake by renal tubular epithelia: studies with cultured OK cells. Am J Physiol Renal Physiol 282 (2002) F465-F471
17. Bode C., Jin H., Rytting E., Silverstein P.S., Young A., and Audus K.L. In vitro models for studying trophoblast transcellular transport. In: Soares M.J., and Hunt J.S. (Eds). Placenta and trophoblast: methods and protocols vol. II (2005), Humana Press, Totowa, NJ
18. Petroff M.G., Phillips T.A., Ka H., Pace J.L., and Hunt J.S. Isolation and culture of term human trophoblast cells. Methods Mol Med 121 (2005) 201-216
19. Rothbard J.B., Jessop T.C., Lewis R.S., Murray B.A., and Wender P.A. Role of membrane potential and hydrogen bonding in the mechanism of translocation of guanidinium-rich peptides into cells. J Am Chem Soc 126 (2004) 9506-9507
20. Koehler M.R., Wissinger B., Gorboulev V., Koepsell H., and Schmid M. The two human organic cation transporter genes SLC22A1 and SLC22A2 are located on chromosome 6q26. Cytogenet Cell Genet 79 (1997) 198-200
21. Bottalico B., Larsson I., Brodszki J., Hernandez-Andrade E., Casslen B., Marsal K., et al. Norepinephrine transporter (NET), serotonin transporter (SERT), vesicular monoamine transporter (VMAT2) and organic cation transporters (OCT1,2 and EMT) in human placenta from pre-eclamptic and normotensive pregnancies. Placenta 25 (2004) 518-529
22. Grundemann D., Harlfinger S., Golz S., Geerts A., Lazar A., Berkels R., et al. Discovery of the ergothioneine transporter. Proc Natl Acad Sci USA 102 (2005) 5256-5261
23. Grundemann D., Hahne C., Berkels R., and Schomig E. Agmatine is efficiently transported by non-neuronal monoamine transporters extraneuronal monoamine transporter (EMT) and organic cation transporter 2 (OCT2). J Pharmacol Exp Ther 304 (2003) 810-817
24. Nakanishi T., Hatanaka T., Huang W., Prasad P.D., Leibach F.H., Ganapathy M.E., et al. Na+- and Cl--coupled active transport of carnitine by the amino acid transporter ATB(0,+) from mouse colon expressed in HRPE cells and Xenopus oocytes. J Physiol 532 (2001) 297-304
25. Sloan J.L., and Mager S. Cloning and functional expression of a human Na(+) and Cl(-)-dependent neutral and cationic amino acid transporter B(0+). J Biol Chem 274 (1999) 23740-23745
26. Toninello A., Salvi M., and Mondovi B. Interaction of biologically active amines with mitochondria and their role in the mitochondrial-mediated pathway of apoptosis. Curr Med Chem 11 (2004) 2349-2374
27. Wu X., Huang W., Prasad P.D., Seth P., Rajan D.P., Leibach F.H., et al. Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter. J Pharmacol Exp Ther 290 (1999) 1482-1492
28. Spalletti-Cernia D., D'Agnano I., Sorrentino R., Zupi G., Vecchio G., Portella G., et al. Verapamil reverts resistance to drug-induced apoptosis in Ki-ras-transformed cells by altering the cell membrane and the mitochondrial transmembrane potentials. Oncol Res 13 (2002) 25-35
29. Burrows K.B., Gudelsky G., and Yamamoto B.K. Rapid and transient inhibition of mitochondrial function following methamphetamine or 3,4-methylenedioxymethamphetamine administration. Eur J Pharmacol 398 (2000) 11-18
30. Virmani A., Gaetani F., Imam S., Binienda Z., and Ali S. Possible mechanism for the neuroprotective effects of l-carnitine on methamphetamine-evoked neurotoxicity. Neuroprotective Agents 993 (2003) 197-207
31. Ward J.L., Sherali A., Mo Z.P., and Tse C.M. Kinetic and pharmacological properties of cloned human equilibrative nucleoside transporters, ENT1 and ENT2, stably expressed in nucleoside transporter-deficient PK15 cells. Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine. J Biol Chem 275 (2000) 8375-8381
32. Mani R.S., Hammond J.R., Marjan J.M., Graham K.A., Young J.D., Baldwin S.A., et al. Demonstration of equilibrative nucleoside transporters (hENT1 and hENT2) in nuclear envelopes of cultured human choriocarcinoma (BeWo) cells by functional reconstitution in proteoliposomes. J Biol Chem 273 (1998) 30818-30825