Fluorescence relaxation in 3D from diffraction-limited sources of PAGFP or sinks of EGFP created by multiphoton photoconversion

Journal of Microscopy

Volumn 225, Issue 1, 2007, Pages 49-71

  Calvert, P D ^a   Peet, J A ^a   Bragin, A ^a   Schiesser, W E ^b,c   Pugh, E N ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c Lehigh University   (United States)

Author keywords

Fluorescence imaging; Multiphoton photoconversion; Multiphoton point spread function; Signal noise ratio in imaging; Two photon imaging

Indexed keywords

CELL MEMBRANES; CYTOLOGY; DIFFRACTION; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; FLUOROPHORES; IMAGE ENHANCEMENT; MOLECULAR BIOLOGY; OPTICAL RESOLVING POWER; PHOTOBLEACHING; PROTEINS; SURFACE DIFFUSION;

FLUORESCENCE IMAGING; MULTIPHOTON PHOTOCONVERSION; MULTIPHOTON POINT-SPREAD FUNCTION; MULTIPHOTONS; PHOTOCONVERSION; POINT-SPREAD FUNCTION; SIGNAL-NOISE RATIO; SIGNAL/NOISE RATIO IN IMAGING; TWO PHOTON IMAGING;

OPTICAL TRANSFER FUNCTION;

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; PHOTOACTIVATABLE GREEN FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; BLEACHING; CELL LINE; CELL MEMBRANE; CHO CELL; CONFIDENCE INTERVAL; DIFFRACTION; DIFFUSION COEFFICIENT; FLUORESCENCE; HYPOTHESIS; IMAGING SYSTEM; LASER; MATHEMATICAL MODEL; MOLECULAR MODEL; MOLECULAR PROBE; MULTIPHOTON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SIGNAL NOISE RATIO; THEORETICAL MODEL; THREE DIMENSIONAL IMAGING; VISCOSITY;

CRICETULUS GRISEUS;

EID: 33846822903     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2007.01715.x     Document Type: Article

References (35)

1. Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E. & Webb, W.W. (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16, 1055-1069.
2. Blonk, J.C.G., Don, A., Vanaalst, H. & Birmingham, J.J. (1993) Fluorescence photobleaching recovery in the confocal scanning light-microscope. J. Microsc. 169, 363-374.
3. Brown, E.B., Wu, E.S., Zipfel, W. & Webb, W.W. (1999) Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery. Biophys. J. 77, 2837-2849.
4. Calvert, P.D., Peet, J.A. & Pugh, E.N. Jr (2005) Through the connecting cilium: protein equilibration between the inner and outer segments of rods observed in real time with photoactivatable GFP. Invest. Ophthalmol. Vis. Sci. 46, 47802.
5. Carslaw, H. S. & Jaegar, J.C. (1959) Conduction of heat in solids. Oxford University Press, Oxford.
6. Chattoraj, M., King, B.A., Bublitz, G.U. & Boxer, S.G. (1996) Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer. Proc. Natl. Acad. Sci. USA 93, 8362-8367.
7. Cone, R.A. (1972) Rotational diffusion of rhodopsin in the visual receptor membrane. Nat. New Biol. 236, 39-43.
8. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A. & Tsien, R.Y. (1995) Under-standing, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455.
9. Denk, W., Piston, D.W. & Webb, W.W. (1995) Two-photon molecular excitation in laser scanning microscopy. Handbook of Biological Confocal Microscopy (ed. by J. B. Pawley), pp. 445-458. Plenum, New York.
10. Dayel, M.J., Hom, E.F. & Verkman, A.S. (1999) Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum. Biophys. J. 76, 2843-2851.
11. Edidin, M. & Fambrough, D. (1973) Fluidity of the surface of cultured muscle fibers. Rapid lateral diffusion of marked surface antigens. J. Cell Biol. 57, 27-37.
12. Elowitz, M.B., Surette, M.G., Wolf, P.E., Stock, J. & Leibler, S. (1997) Photoactivation turns green fluorescent protein red. Curr. Biol. 7, 809-812.
13. Elowitz, M.B., Surette, M.G., Wolf, P.E., Stock, J.B. & Leibler, S. (1999) Protein mobility in the cytoplasm of Escherichia coli. J. Bacteriol. 181, 197-203.
14. Frye, L.D. & Edidin, M. (1970) Rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J. Cell Sci. 7, 319-335.
15. Haggie, P.M. & Verkman, A.S. (2002) Diffusion of tricarboxylic acid cycle enzymes in the mitochondrial matrix in vivo. Evidence for restricted mobility of amultienzyme complex. J. Biol. Chem. 277, 40782-40788.
16. Hollingworth, S., Peet, J., Chandler, W.K. & Baylor, S.M. (2001) Calcium sparks in intact skeletal muscle fibers of the frog. J. Gen. Physiol. 118, 653-678.
17. Kao, H.P. & Verkman, A.S. (1996) Construction and performance of a photobleaching recovery apparatus with microsecond time resolution. Biophys. Chem. 59, 203-210.
18. Kao, H.P., Abney, J.R. & Verkman, A.S. (1993) Determinants of the translational mobility of a small solute in cell cytoplasm. J. Cell Biol. 120, 175-184.
19. Kubitscheck, U., Wedekind, P. & Peters, R. (1998) Three-dimensional diffusion measurements by scanning microphotolysis. J. Microsc. 192, 126-138.
20. Luby-Phelps, K. (2000). Cytoarchitecture and physical properties of the cytoplasm, volume, viscosity, diffusion and intracellular surface area. Int. Rev. Cytol. 192, 189-221.
21. Lukyanov, K.A., Chudakov, D.M., Lukyanov, S. & Verkhusha, V.V. (2005) Innovation: photoactivatable fluorescent proteins. Nat. Rev. Mol. Cell Biol. 6, 885-891.
22. Patterson, G.H. & Lippincott-Schwartz, J. (2002) A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297, 1873-1877.
23. Peet, J.A., Bragin, A., Calvert, P.D., Nikonov, S.S., Mani, S., Zhao, X., Besharse, J.C., Pierce, E.A., Knox, B.E. & Pugh, E.N. Jr (2004) Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptors. J. Cell Sci. 117, 3049-3059.
24. Peters, R., Peters, J., Tews, K.H. & Bahr, W. (1974) A microfluorometric study of translational diffusion in erythrocyte membranes. Biochim. Biophys. Acta. 367, 282-294.
25. Poo, M. & Cone, R.A. (1974) Lateral diffusion of rhodopsin in the photoreceptor membrane. Nature 247, 438-441.
26. Potma, E.O., de Boeij, W.P., Bosgraaf, L., Roelofs, J., van Haastert, P.J. & Wiersma, D.A. (2001) Reduced protein diffusion rate by cytoskeleton in vegetative and polarized dictyostelium cells. Biophys. J. 81, 2010-2019.
27. Schiesser, W.E. (1991) The Numerical Method of Lines: Integration of Partial Differential Equations. Academic Press, San Diego, CA.
28. Shaner, N.C., Steinbach, P.A. & Tsien, R.Y. (2005) A guide to choosing fluorescent proteins. Nat. Methods 2, 905-909.
29. Smith, G.D. (1965) Numerical Solution of Partial Differential Equations. Oxford University Press, Oxford, U.K.
30. Sokolov, M., Lyubarsky, A.L., Strissel, K.J., Savchenko, A.B., Govardovskii, V.I., Pugh, E.N. Jr & Arshavsky, V.Y. (2002) Massive light-driven translocation of transducin between the two major compartments of rod cells: a novel mechanism of light adaptation. Neuron 34, 95-106.
31. Swaminathan, R., Hoang, C.P. & Verkman, A.S. (1997) Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys. J. 72, 1900-1907.
32. Tsien, R.Y. (1998) The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544.
33. Vande Wouwer, A., Saucez, P. & Schiesser, W.E. (2004) Simulation of distributed parameter systems using a Matlab-based method of lines toolbox: chemical engineering applications. Ind. Eng. Chem. Res. 43, 3469-3477.
34. Wei, X., Henke, V.G., Strubing, C., Brown, E.B. & Clapham, D.E. (2003) Real-time imaging of nuclear permeation by EGFP in single intact cells. Biophys. J. 84, 1317-1327.
35. Yokoe, H. & Meyer, T. (1996) Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nat. Biotechnol. 14, 1252-1256.