A novel monoclonal antibody DC63 reveals that inhibitor 1 of protein phosphatase 2A is preferentially nuclearly localised in human brain

FEBS Letters

Volumn 581, Issue 4, 2007, Pages 617-622

  Kovacech, Branislav ^a,b   Kontsekova, Eva ^a   Zilka, Norbert ^a   Novak, Pavol ^a   Skrabana, Rostislav ^a,b   Filipcik, Peter ^a,b   Iqbal, Khalid ^c   Novak, Michal ^a  

^a INSTITUTE OF NEUROIMMUNOLOGY   (Slovakia)

^b Axon Neuroscience Forschungs und Entwicklungs GmbH   (Austria)

^c NEW YORK STATE INSTITUTE FOR BASIC RESEARCH IN DEVELOPMENTAL DISABILITIES   (United States)

Author keywords

Alzheimer's disease; Inhibitor 1 of protein phosphatase 2A; Leucine rich acidic phosphoprotein; Tau protein hyperphosphorylation

Indexed keywords

INHIBITOR 1 OF PROTEIN PHOSPHATASE 2A; INHIBITOR PROTEIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY DC63; UNCLASSIFIED DRUG;

ANTIBODY SPECIFICITY; ARTICLE; BASAL GANGLION; BRAIN; BRAIN NERVE CELL; BRAIN REGION; BRAIN STEM; CEREBELLUM; CONTROLLED STUDY; GLIA CELL; HIPPOCAMPUS; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PARIETAL LOBE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; TEMPORAL CORTEX; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; BRAIN; CELL NUCLEUS; GENE EXPRESSION PROFILING; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN TRANSPORT; PROTEINS; SEQUENCE HOMOLOGY; TISSUE EXTRACTS;

EID: 33846820241     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.01.015     Document Type: Article

References (31)

1. Grundke-Iqbal I., Iqbal K., Tung Y.C., Quinlan M., Wisniewski H.M., and Binder L.I. Abnormal phosphorylation of the microtubule-associated protein tau (tau) in Alzheimer cytoskeletal pathology. Proc. Natl. Acad. Sci. USA 83 (1986) 4913-4917
2. Novak M., Wischik C.M., Edwards P., Pannell R., and Milstein C. Characterisation of the first monoclonal antibody against the pronase resistant core of the Alzheimer PHF. Prog. Clin. Biol. Res. 317 (1989) 755-761
3. Novak M., Kabat J., and Wischik C.M. Molecular characterization of the minimal protease resistant tau unit of the Alzheimer's disease paired helical filament. Embo J. 12 (1993) 365-370
4. Iqbal K., and Grundke-Iqbal I. Metabolic/signal transduction hypothesis of Alzheimer's disease and other tauopathies. Acta Neuropathol. (Berl.) 109 (2005) 25-31
5. Zilka N., et al. Truncated tau from sporadic Alzheimer's disease suffices to drive neurofibrillary degeneration in vivo. FEBS Lett. 580 (2006) 3582-3588
6. Skrabana R., Kontsek P., Mederlyova A., Iqbal K., and Novak M. Folding of Alzheimer's core PHF subunit revealed by monoclonal antibody 423. FEBS Lett. 568 (2004) 178-182
7. Vechterova L., Kontsekova E., Zilka N., Ferencik M., Ravid R., and Novak M. DC11: a novel monoclonal antibody revealing Alzheimer's disease-specific tau epitope. Neuroreport 14 (2003) 87-91
8. Avila J. Tau phosphorylation and aggregation in Alzheimer's disease pathology. FEBS Lett. 580 (2006) 2922-2927
9. Liu F., Grundke-Iqbal I., Iqbal K., and Gong C.X. Contributions of protein phosphatases PP1, PP2A, PP2B and PP5 to the regulation of tau phosphorylation. Eur. J. Neurosci. 22 (2005) 1942-1950
10. Gong C.X., Shaikh S., Wang J.Z., Zaidi T., Grundke-Iqbal I., and Iqbal K. Phosphatase activity toward abnormally phosphorylated tau: decrease in Alzheimer disease brain. J. Neurochem. 65 (1995) 732-738
11. Gong C.X., Singh T.J., Grundke-Iqbal I., and Iqbal K. Phosphoprotein phosphatase activities in Alzheimer disease brain. J. Neurochem. 61 (1993) 921-927
12. Vogelsberg-Ragaglia V., Schuck T., Trojanowski J.Q., and Lee V.M. PP2A mRNA expression is quantitatively decreased in Alzheimer's disease hippocampus. Exp. Neurol. 168 (2001) 402-412
13. Lian Q., Ladner C.J., Magnuson D., and Lee J.M. Selective changes of calcineurin (protein phosphatase 2B) activity in Alzheimer's disease cerebral cortex. Exp. Neurol. 167 (2001) 158-165
14. Li M., Guo H., and Damuni Z. Purification and characterization of two potent heat-stable protein inhibitors of protein phosphatase 2A from bovine kidney. Biochemistry 34 (1995) 1988-1996
15. Li M., Makkinje A., and Damuni Z. Molecular identification of I1PP2A, a novel potent heat-stable inhibitor protein of protein phosphatase 2A. Biochemistry 35 (1996) 6998-7002
16. Tanimukai H., Grundke-Iqbal I., and Iqbal K. Up-regulation of inhibitors of protein phosphatase-2A in Alzheimer's disease. Am. J. Pathol. 166 (2005) 1761-1771
17. Tsujio I., Zaidi T., Xu J., Kotula L., Grundke-Iqbal I., and Iqbal K. Inhibitors of protein phosphatase-2A from human brain structures, immunocytological localization and activities towards dephosphorylation of the Alzheimer type hyperphosphorylated tau. FEBS Lett. 579 (2005) 363-372
18. Chen T.H., Brody J.R., Romantsev F.E., Yu J.G., Kayler A.E., Voneiff E., Kuhajda F.P., and Pasternack G.R. Structure of pp32, an acidic nuclear protein which inhibits oncogene-induced formation of transformed foci. Mol. Biol. Cell. 7 (1996) 2045-2056
19. Brody J.R., Kadkol S.S., Mahmoud M.A., Rebel J.M., and Pasternack G.R. Identification of sequences required for inhibition of oncogene-mediated transformation by pp32. J. Biol. Chem. 274 (1999) 20053-20055
20. Bai J., Brody J.R., Kadkol S.S., and Pasternack G.R. Tumor suppression and potentiation by manipulation of pp32 expression. Oncogene 20 (2001) 2153-2160
21. Beresford P.J., Kam C.M., Powers J.C., and Lieberman J. Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Proc. Natl. Acad. Sci. USA 94 (1997) 9285-9290
22. Vaesen M., Barnikol-Watanabe S., Gotz H., Awni L.A., Cole T., Zimmermann B., Kratzin H.D., and Hilschmann N. Purification and characterization of two putative HLA class II associated proteins: PHAPI and PHAPII. Biol. Chem. H-S 375 (1994) 113-126
23. Ulitzur N., Humbert M., and Pfeffer S.R. Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules. Proc. Natl. Acad. Sci. USA 94 (1997) 5084-5089
24. Ulitzur N., Rancano C., and Pfeffer S.R. Biochemical characterization of mapmodulin, a protein that binds microtubule-associated proteins. J. Biol. Chem. 272 (1997) 30577-30582
25. Itin C., Ulitzur N., Muhlbauer B., and Pfeffer S.R. Mapmodulin, cytoplasmic dynein, and microtubules enhance the transport of mannose 6-phosphate receptors from endosomes to the trans-golgi network. Mol. Biol. Cell 10 (1999) 2191-2197
26. Matilla A., and Radrizzani M. The Anp32 family of proteins containing leucine-rich repeats. Cerebellum 4 (2005) 7-18
27. Csokova N., Skrabana R., Liebig H.D., Mederlyova A., Kontsek P., and Novak M. Rapid purification of truncated tau proteins: model approach to purification of functionally active fragments of disordered proteins, implication for neurodegenerative diseases. Protein Expr. Purif. 35 (2004) 366-372
28. Kontsekova E., Novak M., Kontsek P., Borecky L., and Lesso J. The effect of postfusion cell density on establishment of hybridomas. Folia Biol. (Praha) 34 (1988) 18-22
29. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
30. Iqbal K., et al. Tau pathology in Alzheimer disease and other tauopathies. Biochim. Biophys. Acta 1739 (2005) 198-210
31. Thompson J.D., Higgins D.G., and Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22 (1994) 4673-4680