Novel tissue preservative and tissue fixative for comparative pathology and animal research

Journal of Experimental Animal Science

Volumn 43, Issue 4, 2007, Pages 271-281

  Gugic, Dijana ^a   Nassiri, Mehdi ^a   Nadji, Mehrdad ^a   Morales, Azorides ^a   Vincek, Vladimir ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

Animal pathology; Animal research; Animal tissue; Comparative pathology; Tissue fixative; Tissue preservative UMFIX

Indexed keywords

ALCOHOL; FIXATIVE; FORMALDEHYDE; PARAFFIN; PRESERVATION SOLUTION; RNA;

ANIMAL TISSUE; ANT; ARTICLE; BEETLE; CAT; COMPARATIVE STUDY; CONTROLLED STUDY; DOG; EARTHWORM; EMBEDDING; ENVIRONMENTAL TEMPERATURE; FROZEN SECTION; HIGH TEMPERATURE; HISTOCHEMISTRY; HISTOPATHOLOGY; INTERMETHOD COMPARISON; LIZARD; MORPHOMETRICS; MOUSE; NONHUMAN; PATHOLOGY; PIGEON; QUALITY CONTROL; RABBIT; RAT; RNA DEGRADATION; RNA EXTRACTION; ROOM TEMPERATURE; TISSUE FIXATION; TISSUE PRESERVATION; VETERINARY MEDICINE;

ANIMALIA; CANIS FAMILIARIS; COLEOPTERA; COLUMBA; ORYCTOLAGUS CUNICULUS; RATTUS; SQUAMATA;

EID: 33846794205     PISSN: 09398600     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jeas.2006.09.002     Document Type: Article

References (32)

1. Ahram M., Flaig M.J., Gillespie J.W., Duray P.H., Linehan W.M., Ornstein D.K., Niu S., Zhao Y., Petricoin III E.F., and Emmert-Buck M.R. Evaluation of ethanol-fixed, paraffin-embedded tissues for proteomic applications. Proteomics 3 (2003) 413-421
2. Barceloux D.G., Bond G.R., Krenzelok E.P., Cooper H., and Vale J.A. American Academy of Clinical Toxicology practice guidelines on the treatment of methanol poisoning. J. Toxicol. Clin. Toxicol. 40 (2002) 415-446
3. Blum F. Der formaldehyd als hartungsmittel. Z. Wiss. Microsc. 10 (1893) 314-318
4. Boon M.E., Schmidt U., Cramer-Knijnenburg G.I., and van Krieken J.H. Using Kryofix as alternative for formalin results in more optimal and standardized immunostaining of paraffin sections. Path. Res. Pract. 188 (1992) 832-835
5. Bostwick D.G., Al Annouf N., and Choi C. Establishment of the formalin-free surgical pathology laboratory. Utility of an alcohol-base fixative. Arch. Pathol. Lab. Med. 118 (1994) 298-302
6. Cleary T.J., Morales A.R., Nadji M., Nassiri M., and Vincek V. Antimicrobial activity of UMFIX tissue fixative. J. Clin. Path. 58 (2003) 22-25
7. Cohen C.D., Grone H.J., Grone E.F., Nelson P.J., Schlondorff D., and Kretzler M. Laser microdissection and gene expression analysis on formaldehyde-fixed archival tissue. Kidney Int. 61 (2002) 125-132
8. Eltoum I., Fredenburgh J., Myers R.B., et al. Introduction to the theory and practice of fixation of tissues. J. Histotech. 24 (2001) 173-190
9. Florell S.R., Coffin C.M., Holden J.A., Zimmermann J.W., Gerwels J.W., Summers B.K., Jones D.A., and Leachman S.A. Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod. Pathol. 14 (2001) 116-128
10. Gillespie J.W., Best C.J.M., Bichsel V.E., Cole K.A., Greenhut S.F., Hewitt S.M., Ahram M., Gathright Y.B., Merino M.J., Strausberg R.L., Epstein J.I., Hamilton S.R., Gannot G., Baibakova G.V., Calvert V.S., Flaig M.J., Chuaqui R.F., Herring J.C., Pfeifer J., Petricoin E.F., Linehan W.M., Duray P.H., Bova G.S., and Emmert-Buck M.R. Evaluation of non-formalin tissue fixation for molecular profiling studies. Am. J. Pathol. 160 (2002) 449-457
11. Godfrey T.E., Kim S.H., Chavira M., Ruff D.W., Warren R.S., Gray J.W., and Jensen R.H. Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using nuclease quantitative reverse transcription-polymerase chain reaction. J. Mol. Diagn. 2 (2000) 84-91
12. Goldman T., Wierdon K.H., Kuhl H., Olert J., Branschied D., Pechkovsky D., Zissel G., Galle J., Muller-Quernheim J., and Vollmer E. Assessment of transcriptional gene activity in situ by application of HOPE-fixed, paraffin-embedded tissues. Pathol. Res. Pract. 198 (2002) 91-95
13. Kabbarah O., Pinto K., Mutch D.G., and Goodfellow P.J. Expression profiling of mouse endometrial cancers microdissected from ethanol-fixed, paraffin-embedded tissues. Am. J. Pathol. 162 (2003) 755-762
14. Kilburn K.H., Warshaw R., and Thornthon J.C. Formaldehyde impairs memory, equilibrium and dexterity in histology technicians: effects which persist for days after exposure. Arch. Environ. Health 42 (1987) 117-120
15. Lehmann U., and Kreipe H. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies. Methods 25 (2001) 409-418
16. Marcus S.C., and Feinman S.E. Formaldehyde and cancer epidemiology. In: Feinman S.E. (Ed). Formaldehyde Sensitivity and Toxicity (1988), CRC Press, FL 35-51
17. Masuda N., Ohnoshi T., Kawamoto S., Monden M., and Okubo K. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucl. Acid Res. 27 (1999) 4436-4443
18. Meng L., Lin L., Fresno M., Morales A.R., and Nadji M. Frequency and pattern of p53 gene mutation in a cohort of Spanish women with node-negative breast cancer. Int. J. Oncol. 15 (1999) 555-558
19. Mizuno T., Magamura H., Iwamoto K.S., Ito T., Fukuhara T., Tokunaga M., Tokuoka S., Mabuchi K., and Seyama T. RNA from decades-old archival tissue blocks for retrospective studies. Diagn. Mol. Pathol. 7 (1998) 202-208
20. Morales A.R., Nassiri M., Kanhoush R., Vincek V., and Nadji M. Experience with an automated microwave-assisted rapid tissue processing method. Validation of histologic quality on the timeliness of diagnostic surgical pathology. Am. J. Clin. Pathol. 121 (2004) 528-536
21. Myking A.O., Roynstrand E., and Laerum O.D. Histopathology laboratories. In: Brune D.K., and Edling C. (Eds). Occupational Hazards in Health Professionals (1989), CRC Press, FL 148-161
22. Nadji M., and Morales A.R. Immunoperoxidase: Part I. Technique and its pitfalls. Lab. Med. 14 (1983) 767-771
23. Noguchi M., Furuya S., Taceuchi T., and Hirohashi S. Modified formalin and methanol fixation methods for molecular biological and morphological analysis. Pathol. Int. 47 (1997) 685-691
24. Olert J., Wierdon K.H., Goldman T., Kuhl H., Mehraein Y., Scherthan H., Niketeghad F., Vollmer E., Muller A.M., and Muller-Navia J. HOPE fixation: a novel fixing method and paraffin-embedding technique for human soft tissues. Pathol. Res. Pract. 197 (2001) 823-826
25. Puchtler H., Waldrop F.S., Meloan S.N., Terry M.S., and Conner H.M. Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21 (1970) 97-116
26. Sato Y., Mukai K., Furuya S., and Shimosato Y. The AMeX method: a multipurpose tissue-processing and paraffin-embedding method. III. Extraction and purification of RNA and application to slot-blot hybridization analysis. J. Pathol. 163 (1991) 81-85
27. Specht K., Richter T., Muller U., Walch A., Werner M., and Hofler H. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Am. J. Pathol. 158 (2001) 419-429
28. Srinivasan M., Sedmak D., and Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol. 161 (2002) 1961-1971
29. Tyrrell L., Elias J., and Longley J. Detection of specific mRNAs in routinely processed dermatopathology specimens. Am. J. Dermatopathol. 17 (1995) 476-483
30. Vincek V., Nassiri M., Nadji M., and Morales A.R. A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab. Invest. 83 (2003) 1427-1435
31. Warmington A.R., Wilkinson J.M., and Riley C.B. Evaluation of ethanol-based fixatives as a substitute for formalin in a diagnostic clinical laboratories. J. Histotech. 23 (2000) 299-308
32. Wester K., Asplund A., Backvall H., Micke P., Derveniece A., Hartmane I., Malmstrom P.U., and Ponten F. Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing. Lab. Invest. 83 (2003) 889-899