Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method

Genome Research

Volumn 11, Issue 9, 2001, Pages 1553-1558

  Piao, Y ^a   Ko, N T ^a   Lim, M K ^a   Ko, M S H ^a  

^a NATIONAL INSTITUTE ON AGING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; COMPLEMENTARY DNA; GUANINE; PRIMER DNA; RNA; TYROSINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; CONTROLLED STUDY; DNA LIBRARY; EMBRYO; GENE AMPLIFICATION; GENE EXPRESSION; GENE INSERTION; GENE ISOLATION; GENETIC TRANSCRIPTION; KIDNEY; MOLECULAR CLONING; MOUSE; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; START CODON; UNINDEXED SEQUENCE;

ANIMALS; DNA, COMPLEMENTARY; GENE AMPLIFICATION; GENE LIBRARY; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA;

EID: 0034835934     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.185501     Document Type: Article

References (21)

1. PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates
2. Homing endonucleases: Keeping the house in order
3. PCR-based cDNA library construction: General cDNA libraries at the level of a few cells
4. ESTablishing a human transcript map
5. High-efficiency full-length cDNA cloning by biotinylated CAP trapper
6. Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes
7. High efficiency selection of full-length cDNA by improved biotinylated cap trapper
8. Effective amplification of long targets from cloned inserts and human genomic DNA
9. An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture)
10. Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development
11. Unbiased amplification of a highly complex mixture of DNA fragments by 'lone linker'-tagged PCR
12. The I.M.A.G.E. Consortium: An integrated molecular analysis of genomes and their expression
13. An encyclopedia of mouse genes
14. Oligo-capping: A simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides
15. An improved method for construction of directionally cloned cDNA libraries from microdissected cells
16. The mammalian gene collection
17. Statistical analysis of the 5′ untranslated region of human mRNA using "Oligo-Capped" cDNA libraries
18. Construction and characterization of a full length-enriched and a 5′-end-enriched cDNA library
19. Toward a whole cDNA catalog: Construction of an equalized cDNA library from mouse embryos
20. Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray
21. Optimizing utilization of DNA from rare or archival anthropological samples