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X-ray absorption spectroscopic (XAS) measurements were conducted at the Stanford Synchrotron Radiation Laboratory with the SPEAR 3 storage ring containing between 85 and 100 mA at 3.0 GeV. Data were collected on the structural molecular biology XAS beamline 9-3 with a wiggler field of 2 T and employing a Si(220) double-crystal monochromator. Beamline 9-3 is equipped with a rhodium-coated vertical collimating mirror upstream of the monochromator and a downstream bent-cylindrical focusing mirror also rhodium-coated, Harmonic rejection was accomplished by setting the cutoff angle of the mirrors to 23 keV. Incident and transmitted X-ray intensities were monitored using argon-filled ionization chambers, and X-ray absorption was measured as the Mo Ka fluorescence excitation spectrum using an array of 30 germanium detectors.11 During data collection, samples were maintained at a temperature of approximately 10 K using an Oxford Instruments liquid-helium-flow cryostat. Ten 40-min
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17 Samples were prepared in a buffer containing 100 mM 3-(N-morpholino)propanesulfonic acid and 5 mM ethylenediamine-tetraacetic acid (pH 7).
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17 Samples were prepared in a buffer containing 100 mM 3-(N-morpholino)propanesulfonic acid and 5 mM ethylenediamine-tetraacetic acid (pH 7).
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For example, see
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This can be estimated using Fourier filtering techniques
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This can be estimated using Fourier filtering techniques.
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20
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