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Volumn 46, Issue 1, 2007, Pages 2-4

X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase

Author keywords

[No Author keywords available]

Indexed keywords

DIMETHYL SULFOXIDE REDUCTASE; IRON SULFUR PROTEIN; MOLYBDENUM; OXIDOREDUCTASE;

EID: 33846464982     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic061695t     Document Type: Article
Times cited : (21)

References (20)
  • 10
    • 33846443401 scopus 로고    scopus 로고
    • X-ray absorption spectroscopic (XAS) measurements were conducted at the Stanford Synchrotron Radiation Laboratory with the SPEAR 3 storage ring containing between 85 and 100 mA at 3.0 GeV. Data were collected on the structural molecular biology XAS beamline 9-3 with a wiggler field of 2 T and employing a Si(220) double-crystal monochromator. Beamline 9-3 is equipped with a rhodium-coated vertical collimating mirror upstream of the monochromator and a downstream bent-cylindrical focusing mirror also rhodium-coated, Harmonic rejection was accomplished by setting the cutoff angle of the mirrors to 23 keV. Incident and transmitted X-ray intensities were monitored using argon-filled ionization chambers, and X-ray absorption was measured as the Mo Ka fluorescence excitation spectrum using an array of 30 germanium detectors.11 During data collection, samples were maintained at a temperature of approximately 10 K using an Oxford Instruments liquid-helium-flow cryostat. Ten 40-min
    • 15
  • 16
    • 33846428204 scopus 로고    scopus 로고
    • 17 Samples were prepared in a buffer containing 100 mM 3-(N-morpholino)propanesulfonic acid and 5 mM ethylenediamine-tetraacetic acid (pH 7).
    • 17 Samples were prepared in a buffer containing 100 mM 3-(N-morpholino)propanesulfonic acid and 5 mM ethylenediamine-tetraacetic acid (pH 7).
  • 19
    • 33846458774 scopus 로고    scopus 로고
    • This can be estimated using Fourier filtering techniques
    • This can be estimated using Fourier filtering techniques.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.