Effects of severe hypocapnia on expression of Bax and Bcl-2 proteins, DNA fragmentation, and membrane peroxidation products in cerebral cortical mitochondria of newborn piglets

Neonatology

Volumn 91, Issue 1, 2007, Pages 20-27

  Pirot, Anayansi Lasso ^a   Fritz, Karen I ^b   Ashraf, Qazi M ^b   Mishra, Om P ^b   Delivoria Papadopoulos, Maria ^b  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b ST CHRISTOPHER'S HOSPITAL FOR CHILDREN   (United States)

Author keywords

Apoptosis; Brain injury; Mitochondria; Ventilation

Indexed keywords

ADENOSINE TRIPHOSPHATE; CREATINE PHOSPHATE; DNA FRAGMENT; MEMBRANE LIPID; MITOCHONDRIAL DNA; PROTEIN ANTIBODY; PROTEIN BAX; PROTEIN BCL 2;

ANESTHESIA; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTERIAL CARBON DIOXIDE TENSION; ARTICLE; ASSISTED VENTILATION; BRAIN CORTEX; BRAIN INJURY; BRAIN MITOCHONDRION; CELL DEATH; CELL MEMBRANE; CHEMOLUMINESCENCE; DENSITOMETRY; DNA DAMAGE; DNA ISOLATION; HYPOCAPNIA; IMAGING SYSTEM; LIPID PEROXIDATION; MITOCHONDRIAL MEMBRANE; NEWBORN; NONHUMAN; PIGLET; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; SPECTROFLUOROMETRY; STATISTICAL SIGNIFICANCE;

ANIMALS; ANIMALS, NEWBORN; BCL-2-ASSOCIATED X PROTEIN; CEREBRAL CORTEX; DNA FRAGMENTATION; DNA, MITOCHONDRIAL; HYPOCAPNIA; LIPID PEROXIDATION; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; SWINE;

EID: 33846464260     PISSN: 16617800     EISSN: 16617819     Source Type: Journal    
DOI: 10.1159/000096967     Document Type: Article

References (56)

1. Darby JM, Yonas H, Marion DW, Latchaw RE: Local 'inverse steal' induced by hyperventilation in head injury. Neurosurgery 1988;23:84-88.
2. Marion DW, Firlik A, McLaughlin M: Hyperventilation therapy for severe traumatic brain injury. New Horiz 1995;3:439-447.
3. Drummond WH, Gregory GA, Heymann MA, Phibbs RA: The independent effects of hyperventilation, tolazoline, and dopamine on infants with persistent pulmonary hypertension. J Pediatr 1981;98:603-611.
4. Amato MBP, Barbas CSV, Medeiros DM, Magaldi RB, Schettino GP, Lorenzi-Filho G, Kairalla RA, Deheinzelin D, Munoz C, Oliveira R, Takagaki TY, Carvalho CR: Effect of a protective-ventilation strategy on mortality in the acute respiratory distress syndrome. N Engl J Med 1998;338:347-354.
5. Riphagen S, Bohn D: High frequency oscillatory ventilation. Intensive Care Med 1999; 25:1459-1462.
6. Murase M, Ishida A: Early hypocarbia of preterm infants: its relationship to periventricular leukomalacia and cerebral palsy, and its perinatal risk factors. Acta Paediatr 2005;94:85-91.
7. Nevin M, Colchester AC, Adams S, Pepper JR: Evidence for involvement of hypocapnia and hypoperfusion in aetiology of neurological deficit after cardiopulmonary bypass. Lancet 1987;ii:1493-1495.
8. Graziani LJ, Gringlas M, Baumgart S: Cerebrovascular complications and neurodevelopmental sequelae of neonatal ECMO. Clin Perinatol 1997;24:655-675.
9. O'Cain CF, Hensley MJ, McFadden ER Jr, Ingram RH Jr: Pattern and mechanism of airway response to hypocapnia in normal subjects. J Appl Physiol 1979;47:8-12.
10. A/Q heterogeneity in canine pulmonary edema. Anesthesiology 1993;78:91-99.
11. 2 gradient as an indicator of splanchnic perfusion during systemic hypo- and hypercapnia. Crit Care Med 1999;27:2760-2765.
12. Laffey JG, Kavanagh BP: Hypocapnia. N Engl J Med 2002;347:43-53.
13. Greisen G, Munck H, Lou H: Severe hypocarbia in preterm infants and neurodevelopmental deficit. Acta Paediatr Scan 1987;76:401-404.
14. Liao SL, Lai SH, Chou YH,Kuo CY: Effect of hypocapnia in the first three days of life on the subsequent development of periventricular leukomalacia in premature infants. Acta Paediatr Taiwan 2001;42:90-93.
15. Giannakopoulou C, Korakaki E, Manoura A, Bikouvarakis S, Papageorgiou M, Gourgiotis D, Hatzidaki E: Significance of hypocarbia in the development of periventricular leukomalacia in preterm infants. Pediatr Int 2004;46:268-273.
16. Fritz KI, Ashraf QM, Mishra OP, Delivoria-Papadopoulos M: Effect of moderate hypocapnic ventilation on nuclear DNA fragmentation and energy metabolism in the cerebral cortex of newborn piglets. Pediatr Res 2001;50:586-589.
17. 2 27 mm Hg) on CaM kinase IV activity, Bax/bcl-2 protein expression and DNA fragmentation in the cerebral cortex of newborn piglets. Neurosci Lett 2003;352:211-215.
18. Compton MM: Development of an apoptosis endonuclease assay. DNA Cell Biol 1991;10:133-141.
19. Chao DT, Korsemeyer SJ: Bcl-2 family: regulators of cell death. Annu Rev Immunol 1998;16:395-419.
20. Clair EGS, Anderson SJ, Oltvai ZN: Bcl-2 counters apoptosis by Bax heterodimerization-dependent and -independent mechanisms in the T-cell lineage. J Biol Chem 1997;272:29347-29355.
21. Ravagnan L, Roumier T, Kroemer G: Mitochondria, the killer organelles and their weapons. J Cell Physiol 2002;192:131-137.
22. Jacobson MD, Burne JF, Raff MC: Programmed cell death and Bcl-2 protection in the absence of a nucleus. EMBO J 1994;13:1899-1910.
23. Schulze-Osthoff K, Walczak H, Droge W, Krammer PH: Cell nucleus and DNA fragmentation are not required for apoptosis. J Cell Biol 1994;127:15-20.
24. Putcha GV, Deshmukh M, Johnson EM Jr: BAX translocation is a critical event in neuronal apoptosis: regulation by neuroprotectants, Bcl-2, and caspases. J Neurosci 1999;19:7476-7485.
25. Finkel E: The mitochondrion: Is it central to apoptosis? Science 2001;292:624-626.
26. Sastry PS, Rao KS: Apoptosis and the nervous system. J Neurochem 2000;74:1-20.
27. Hara A, Iwai, T, Niwa M, Umetsu T, Yoshimi N, Tanaka T, Mori H: Immunohistochemical detection of Bax and Bcl-2 proteins in gerbil hippocampus following transient forebrain ischemia. Brain Res 1996;711:249-253.
28. Reed JC: Bcl-2 and the regulation of programmed cell death. J Cell Biol 1994;124:1-6.
29. Rosenbaum DM, Michaelson M, Batter DK, Doshi P, Kessler JA: Evidence for hypoxia-induced, programmed cell death of culture neurons. Ann Neurol 1994;36:864-870.
30. Chen J, Graham SH, Chan PH, Lan JQ, Zhou GJ, Simon RP: Bcl-2 is expressed in neurons that survive focal ischemia in rat. Neuroreport 1995;6:394-398.
31. Jacobson MD, Raff MC: Programmed cell death and Bcl-2 protection in very low oxygen. Nature 1995;374:814.
32. Martinou JC, Dubois-Dauphin M, Staple JK, Rodriguez I, Frankowski H, Missotten M, Albertini P, Talabot D, Catsicas S, Pietra C, Huarte J: Overexpression of Bcl-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia. Neuron 1994;13:1017-1030.
33. Zhong LT, Sarafian T, Kane DJ, Charles AC, Mah SP, Edwards RH, Bredesen DE: Bcl-2 inhibits death of central neural cells induced by multiple agents. Proc Natl Acad Sci USA 1993;90:4533-4537.
34. Chinnaiyan AM, O'Rourke K, Lane BR, Dixit VM: Interaction of CED-4 with CED-3 and CED-9: a molecular framework for cell death. Science 1997;275:1122-1126.
35. Golstein P: Controlling cell death. Science 1997;275:1081-1082.
36. Krajewski S, Mal JK, Krejewski M, Sikorska M, Mossakowski MJ, Reed JC: Upregulation of Bax protein levels in neurons following cerebral ischemia. J Neurosci 1995;15:6364-6376.
37. Lok J, Martin LJ: Rapid subcellular redistribution of Bax precedes caspase-3 and endonuclease activation during excitotoxic neuronal apoptosis in rat brain. J Neurotrauma 2002;19:815-828.
38. Plesnila N: Role of mitochondrial proteins for neuronal cell death after focal cerebral ischemia. Acta Neuorchir Suppl 2004;89:15-19.
39. Watson BD: Evaluation of the concomitance of lipid peroxidation in experimental models of cerebral ischemia and stroke. Progress Brain Res 1993;96:69-95.
40. Yeagle PL: Lipid regulation of cell membrane structure and function. FASEB J 1989;3:1833-1842.
41. Vanucci RC: Experimental biology of cerebral hypoxia-ischemia: relation to perinatal brain damage. Pediatr Res 1990;27:317-326.
42. Lamprecht W, Stein P, Heinz F, Weissner H: Creatine phosphate; in Bergmeyer HU (ed): Methods of Enzymatic Analysis. New York, Academic Press, 1974, pp 1777-1781.
43. Booth RFG, Clark JB: A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain. Biochem J 1978;176:365-370.
44. Lowry O, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275.
45. Higuchi Y, Linn S: Purification of all forms of Hela cell mitochondrial DNA and assessment of damage to it caused by hydrogen peroxide treatment of mitochondria or cells. J Biol Chem 1995;270:7950-7956.
46. Folch J, Lees MJ, Sloane-Stanley GH: A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 1957;226:497-509.
47. Recknagel RO, Glende EA: Spectrophotometric detection of lipid conjugated dienes. Methods Enzymol 1984;105:331-337.
48. Gillardon F, Lenz C, Kuschinsky W, Zimmermann M: Evidence for apoptotic cell death in the choroid plexus following focal cerebral ischemia. Neurosci Lett 1996;207:113-116.
49. Graham EM, Apostlou M, Mishra OP, Delivoria-Papadopouos M: Modification of the N-methyl-D-aspartate (NMDA) receptor in the brain of newborn piglets following hyperventilation induced ischemia. Neurosci Lett 1996;218:29-32.
50. Wilson DF, Pastuszko A, DiGiacomo JE, Pawlowski M, Schneiderman R, Delivoria-Papadopoulos M: Effect of hyperventilation on oxygenation of the brain cortex of newborn piglets. J Appl Physiol 1991;70:2691-2696.
51. Delivoria-Papadopoulos M, Mishra OP: Nuclear mechanisms of hypoxic cerebral injury in the newborn. Clin Perinatol 2004;31:91-105.
52. van Bel F, van de BM, Baan J, Ruys JH: The influence of abnormal blood gases on cerebral blood flow velocity in the preterm newborn. Neuropediatrics 1988;19:27-32.
53. Brown DW, Picot PA, Naeini JG, Springett R, Delpy DT, Lee TY: Quantitative near infrared spectroscopy measurement of cerebral hemodynamics in newborn piglets. Ped Res 2002;51:564-570.
54. Huttunen J, Tolvanen H, Heinonen E, Voipio J, Wikstrom H, Ilmoniemi RJ, Hari R, Kaila K: Effects of voluntary hyperventilation on cortical sensory responses. Electroencephalographic and magnetoencephalographic studies. Exp Brain Res 1999;125:248-254.
55. Vannucci RC, Brucklacher RM, Vannucci SJ: Effect of carbon dioxide on cerebral metabolism during hypoxia-ischemia in the immature rat. Pediatr Res 1997;42:24-29.
56. van Hulst RA, Lameris TW, Haitsma JJ, Klein J, Lachmann B: Brain glucose and lactate levels during ventilator-induced hypo-and hypercapnia. Clin Physiol Func Imaging 2004;24:243-248.