Multiplex detection of Enterocytozoon bieneusi and Encephalitozoon spp. in fecal samples using real-time PCR

Diagnostic Microbiology and Infectious Disease

Volumn 57, Issue 2, 2007, Pages 163-167

  Verweij, Jaco J ^a   ten Hove, Robert ^a   Brienen, Eric A T ^a   van Lieshout, Lisette ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Encephalitozoon spp.; Enterocytozoon bieneusi; Real time PCR diarrhea

Indexed keywords

DNA;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; ENCEPHALITOZOON; ENTEROCYTOZOON; FECES ANALYSIS; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; DNA, PROTOZOAN; ENCEPHALITOZOON; ENCEPHALITOZOONOSIS; ENTEROCYTOZOON; FECES; HUMANS; MICROSPORIDIOSIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 33846357451     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2006.08.009     Document Type: Article

References (32)

1. Didier E.S. Microsporidiosis: an emerging and opportunistic infection in humans and animals. Acta Trop. 94 (2005) 61-76
2. Franzen C., and Muller A. Molecular techniques for detection, species differentiation, and phylogenetic analysis of microsporidia. Clin. Microbiol. Rev. 12 (1999) 243-285
3. Gainzarain J.C., Canut A., Lozano M., Labora A., Carreras F., Fenoy S., Navajas R., Pieniazek N.J., Da Silva A.J., and del Aguila C. Detection of Enterocytozoon bieneusi in two human immunodeficiency virus-negative patients with chronic diarrhea by polymerase chain reaction in duodenal biopsy specimens and review. Clin. Infect. Dis. 27 (1998) 394-398
4. Garcia L.S. Laboratory identification of the microsporidia. J. Clin. Microbiol. 40 (2002) 1892-1901
5. Ghosh S., Debnath A., Sil A., De S., Chattopadhyay D.J., and Das P. PCR detection of Giardia lamblia in stool: targeting intergenic spacer region of multicopy rRNA gene. Mol. Cell. Probes 14 (2000) 181-189
6. Gomez Morales M.A., Atzori C., Ludovisi A., Rossi P., Scaglia M., and Pozio E. Opportunistic and non-opportunistic parasites in HIV-positive and negative patients with diarrhoea in Tanzania. Trop. Med. Parasitol. 46 (1995) 109-114
7. Katzwinkel-Wladarsch S., Lieb M., Helse W., Loscher T., and Rinder H. Direct amplification and species determination of microsporidian DNA from stool specimens. Trop. Med. Int. Health 1 (1996) 373-378
8. Klein D. Quantification using real-time PCR technology: applications and limitations. Trends Mol. Med. 8 (2002) 257-260
9. Kock N.P., Petersen H., Fenner T., Sobottka I., Schmetz C., Deplazes P., Pieniazek N.J., Albrecht H., and Schottelius J. Species-specific identification of microsporidia in stool and intestinal biopsy specimens by the polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis. 16 (1997) 369-376
10. Leelayoova S., Subrungruang I., Rangsin R., Chavalitshewinkoon-Petmitr P., Worapong J., Naaglor T., and Mungthin M. Transmission of Enterocytozoon bieneusi genotype a in a Thai orphanage. Am. J. Trop. Med. Hyg. 73 (2005) 104-107
11. Lopez-Velez R., Turrientes M.C., Garron C., Montilla P., Navajas R., Fenoy S., and del Aguila C. Microsporidiosis in travelers with diarrhea from the tropics. J. Travel Med. 6 (1999) 223-227
12. Lores B., Lopez-Miragaya I., Arias C., Fenoy S., Torres J., and del Aguila C. Intestinal microsporidiosis due to Enterocytozoon bieneusi in elderly human immunodeficiency virus-negative patients from Vigo, Spain. Clin. Infect. Dis. 34 (2002) 918-921
13. Menotti J., Cassinat B., Porcher R.L., Sarfati C., Derouin F., and Molina J.M. Development of a real-time polymerase-chain-reaction assay for quantitative detection of Enterocytozoon bieneusi DNA in stool specimens from immunocompromised patients with intestinal microsporidiosis. J. Infect. Dis. 187 (2003) 1469-1474
14. Menotti J., Cassinat B., Sarfati C., Liguory O., Derouin F., and Molina J.M. Development of a real-time PCR assay for quantitative detection of Encephalitozoon intestinalis DNA. J. Clin. Microbiol. 41 (2003) 1410-1413
15. Monteiro L., Bonnemaison D., Vekris A., Petry K.G., Bonnet J., Vidal R., Cabrita J., and Megraud F. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J. Clin. Microbiol. 35 (1997) 995-998
16. Morgan U.M., Pallant L., Dwyer B.W., Forbes D.A., Rich G., and Thompson R.C. Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial. J. Clin. Microbiol. 36 (1998) 995-998
17. Muller A., Bialek R., Kamper A., Fatkenheuer G., Salzberger B., and Franzen C. Detection of microsporidia in travelers with diarrhea. J. Clin. Microbiol. 39 (2001) 1630-1632
18. Mungthin M., Subrungruang I., Naaglor T., Aimpun P., Areekul W., and Leelayoova S. Spore shedding pattern of Enterocytozoon bieneusi in asymptomatic children. J. Med. Microbiol. 54 (2005) 473-476
19. Niesters H.G. Clinical virology in real time. J. Clin. Virol. 25 (2002) S3-S12
20. Notermans D.W., Peek R., de Jong M.D., Wentink-Bonnema E.M., Boom R., and van Gool T. Detection and identification of Enterocytozoon bieneusi and Encephalitozoon species in stool and urine specimens by PCR and differential hybridization. J. Clin. Microbiol. 43 (2005) 610-614
21. Raynaud L., Delbac F., Broussolle V., Rabodonirina M., Girault V., Wallon M., Cozon G., Vivares C.P., and Peyron F. Identification of Encephalitozoon intestinalis in travelers with chronic diarrhea by specific PCR amplification. J. Clin. Microbiol. 36 (1998) 37-40
22. Subrungruang I., Mungthin M., Chavalitshewinkoon-Petmitr P., Rangsin R., Naaglor T., and Leelayoova S. Evaluation of DNA extraction and PCR methods for detection of Enterocytozoon bienuesi in stool specimens. J. Clin. Microbiol. 42 (2004) 3490-3494
23. van Gool T., Canning E.U., and Dankert J. An improved practical and sensitive technique for the detection of microsporidian spores in stool samples. Trans. R. Soc. Trop. Med. Hyg. 88 (1994) 189-190
24. Verweij J.J., Pit D.S.S., Van Lieshout L., Baeta S.M., Dery G.D., Gasser R.B., and Polderman A.M. Determining the prevalence of Oesophagostomum bifurcum and Necator americanus infections using specific PCR amplification of DNA from faecal samples. Trop. Med. Int. Health 6 (2001) 726-731
25. Verweij J.J., Laeijendecker D., Brienen E.A.T., Van Lieshout L., and Polderman A.M. Detection of Cyclospora cayetanensis in travellers returning from the tropics and subtropics using microscopy and real-time PCR. Int. J. Med. Microbiol. 293 (2003) 199-202
26. Verweij J.J., Oostvogel F., Brienen E.A.T., Nang-Neifubah A., Ziem J., and Polderman A.M. Prevalence of Entamoeba histolytica and Entamoeba dispar in northern Ghana. Trop. Med. Int. Health 8 (2003) 1153-1156
27. Verweij J.J., Schinkel J., Laeijendecker D., van Rooyen M.A.A., Van Lieshout L., and Polderman A.M. Real-time PCR for the detection of Giardia lamblia. Mol. Cell. Probes 17 (2003) 223-225
28. Verweij J.J., Blange R.A., Templeton K., Schinkel J., Brienen E.A.T., van Rooyen M.A.A., Van Lieshout L., and Polderman A.M. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J. Clin. Microbiol. 42 (2004) 1220-1223
29. Wanke C.A., DeGirolami P., and Federman M. Enterocytozoon bieneusi infection and diarrheal disease in patients who were not infected with human immunodeficiency virus: case report and review. Clin. Infect. Dis. 23 (1996) 816-818
30. Webster K.A., Smith H.V., Giles M., Dawson L., and Robertson L.J. Detection of Cryptosporidium parvum oocysts in faeces: comparison of conventional coproscopical methods and the polymerase chain reaction. Vet. Parasitol. 61 (1996) 5-13
31. Wichro E., Hoelzl D., Krause R., Bertha G., Reinthaler F., and Wenisch C. Microsporidiosis in travel-associated chronic diarrhea in immune-competent patients. Am. J. Trop. Med. Hyg. 73 (2005) 285-287
32. Wolk D.M., Schneider S.K., Wengenack N.L., Sloan L.M., and Rosenblatt J.E. Real-time PCR method for detection of Encephalitozoon intestinalis from stool specimens. J. Clin. Microbiol. 40 (2002) 3922-3928