High-risk HPV E6 oncoproteins assemble into large oligomers that allow localization of endogenous species in prototypic HPV-transformed cell lines

Biochemistry

Volumn 46, Issue 2, 2007, Pages 341-349

  García Alai, María M ^a   Dantur, Karina I ^a   Smal, Clara ^a   Pietrasanta, Lía ^b   De Prat Gay, Gonzalo ^a  

^a FUNDACIÓN INSTITUTO LELOIR   (Argentina)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BIODEGRADATION; CELLS; MONOMERS; OLIGOMERS; ONCOLOGY;

CARCINOGENIC PROGRESSION; CERVICAL CANCER; ENDOGENOUS PROTEIN; UBIQUITIN-PROTEASOME DEGRADATION;

PROTEINS;

OLIGOMER; PDZ PROTEIN; PROTEASOME; PROTEIN ANTIBODY; PROTEIN E6; PROTEIN P53; RECOMBINANT PROTEIN; UBIQUITIN;

ARTICLE; CANCER GROWTH; CARCINOGENESIS; CELL LINE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DNA TUMOR VIRUS; EMBRYO; HELA CELL; HUMAN; HUMAN CELL; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN TARGETING; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT; SERUM; STRUCTURAL PROTEOMICS; THERMOSTABILITY; UTERINE CERVIX CANCER; VIRUS CELL TRANSFORMATION; VIRUS STRAIN; VIRUS TYPING; WART VIRUS;

BASE SEQUENCE; CELL LINE, TRANSFORMED; CELL TRANSFORMATION, NEOPLASTIC; CELL TRANSFORMATION, VIRAL; DNA, VIRAL; DNA-BINDING PROTEINS; FEMALE; HUMAN PAPILLOMAVIRUS 16; HUMAN PAPILLOMAVIRUS 18; HUMANS; MODELS, BIOLOGICAL; MULTIPROTEIN COMPLEXES; ONCOGENE PROTEINS, VIRAL; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; REPRESSOR PROTEINS; TUMOR SUPPRESSOR PROTEIN P53; UTERINE CERVICAL NEOPLASMS;

HUMAN PAPILLOMAVIRUS;

EID: 33846238969     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi602457q     Document Type: Article

References (33)

1. Lowy, D. R., and Howley, P. M. (2001) in Fields Virology (Fields, B. N., Howley, P. M., Griffin, D. E., Lamb, R. A., Martin, M. A., Roizman, B., Straus, S. E., and Knipe, D. M., Eds.) pp 2213-2219, Lippincott Williams and Wilkins, Philadelphia.
2. Thomas, M., Pim, D., and Banks, L. (2006) in Papillomavirus Research (Campo, M. S., Ed.) pp 115-131, Caister Academic Press, Wymondham, Norfolk, U.K.
3. Helt, A. M., and Galloway, D. A. (2003) Mechanisms by which DNA tumor virus oncoproteins target the Rb family of pocket proteins, Carcinogenesis 24, 159-169.
4. Zambetti, G. P. (2005) in Protein Reviews (Atassi, M. Z., Ed.) pp 1-23, Springer Science, New York.
5. Munger, K., Baldwin, A., Edwards, K. M., Hayakawa, H., Nguyen, C. L., Owens, M., Grace, M., and Huh, K. (2004) Mechanisms of human papillomavirus-induced oncogenesis, J. Virol. 78, 11451-11460.
6. Munger, K., and Howley, P. M. (2002) Human papillomavirus immortalization and transformation functions, Virus Res. 89, 213-228.
7. Durst, M., Gissmann, L., Ikenberg, H., and zur Hausen, H. (1983) A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions, Proc. Natl. Acad. Sci. U.S.A. 80, 3812-3815.
8. Yang, Y. C., Spalholz, B. A., Rabson, M. S., and Howley, P. M. (1985) Dissociation of transforming and trans-activation functions for bovine papillomavirus type 1, Nature 318, 575-577.
9. Soeda, E., Ferran, M. C., Baker, C. C., and McBride, A. A. (2006) Repression of HPV16 early region transcription by the E2 protein, Virology (in press).
10. Mantovani, F., and Banks, L. (2001) The human papillomavirus E6 protein and its contribution to malignant progression, Oncogene 20, 7874-7887.
11. Munger, K., Phelps, W. C., Bubb, V., Howley, P. M., and Schlegel, R. (1989) The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes, J. Virol. 63, 4417-4421.
12. Syrjänen, S. (2005) HPV Today, pp 8-10, BYPASS Ediciones, Madrid.
13. Simonson, S. J., Difilippantonio, M. J., and Lambert, P. F. (2005) Two distinct activities contribute to human papillomavirus 16 E6's oncogenic potential, Cancer Res. 65, 8266-8273.
14. Kiyono, T., Foster, S. A., Koop, J. I., McDougall, J. K., Galloway, D. A., and Klingelhutz, A. J. (1998) Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells, Nature 396, 84-88.
15. Barbosa, M. S., Lowy, D. R., and Schiller, J. T. (1989) Papillomavirus polypeptides E6 and E7 are zinc-binding proteins, J. Virol. 63, 1404-1407.
16. Huibregtse, J. M., Scheffner, M., Beaudenon, S., and Howley, P. M. (1995) A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase, Proc. Natl. Acad. Sci. U.S.A. 92, 5249.
17. Nomine, Y., Masson, M., Charbonnier, S., Zanier, K., Ristriani, T., Deryckere, F., Sibler, A. P., Desplancq, D., Atkinson, R. A., Weiss, E., Orfanoudakis, G., Kieffer, B., and Trave, G. (2006) Structural and functional analysis of E6 oncoprotein: Insights inthe molecular pathways of human papillomavirus-mediated pathogenesis, Mol. Cell 21, 665-678.
18. Daniels, P. R., Sanders, C. M., and Maitland, N. J. (1998) Characterization of the interactions of human papillomavirus type 16 E6 with p53 and E6-associated protein in insect and human cells, J. Gen. Virol. 79 (Part 3), 489-499.
19. Liang, X. H., Volkmann, M., Klein, R., Herman, B., and Lockett, S. J. (1993) Co-localization of the tumor-suppressor protein p53 and human papillomavirus E6 protein in human cervical carcinoma cell lines, Oncogene 8, 2645-2652.
20. Kanda, T., Watanabe, S., Zanma, S., Sato, H., Furuno, A., and Yoshiike, K. (1991) Human papillomavirus type 16 E6 proteins with glycine substitution for cysteine in the metal-binding motif, Virology 185, 536-543.
21. Masson, M., Hindelang, C., Sibler, A. P., Schwalbach, G., Trave, G., and Weiss, E. (2003) Preferential nuclear localization of the human papillomavirus type 16 E6 oncoprotein in cervical carcinoma cells, J. Gen. Virol. 84, 2099-2104.
22. Guccione, E., Massimi, P., Bernat, A., and Banks, L. (2002) Comparative analysis of the intracellular location of the high- and low-risk human papillomavirus oncoproteins, Virology 293, 20-25.
23. Alonso, L. G., Garcia-Alai, M. M., Smal, C., Centeno, J. M., Iacono, R., Castano, E., Gualfetti, P., and de Prat-Gay, G. (2004) The HPV16 E7 viral oncoprotein self-assembles into defined spherical oligomers, Biochemistry 43, 3310-3317.
24. Schwarz, E., Freese, U. K., Gissmann, L., Mayer, W., Roggenbuck, B., Stremlau, A., and zur Hausen, H. (1985) Structure and transcription of human papillomavirus sequences in cervical carcinoma cells, Nature 314, 111-114.
25. Uversky, V. N. (2002) Natively unfolded proteins: A point where biology waits for physics, Protein Sci. 11, 739-756.
26. Nomine, Y., Ristriani, T., Laurent, C., Lefevre, J. F., Weiss, E., and Trave, G. (2001) Formation of soluble inclusion bodies by hpv e6 oncoprotein fused to maltose-binding protein, Protein Expression Purif. 23, 22-32.
27. Nomine, Y., Ristriani, T., Laurent, C., Lefevre, J. F., Weiss, E., and Trave, G. (2001) A strategy for optimizing the monodispersity of fusion proteins: Application to purification of recombinant HPV E6 oncoprotein, Protein Eng. 14, 297-305.
28. 28.Cole, S. T., and Danos, O. (1987) Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products, J. Mol. Biol. 193, 599-608.
29. Degenkolbe, R., Gilligan, P., Gupta, S., and Bernard, H. U. (2003) Chelating agents stabilize the monomeric state of the zinc binding human papillomavirus 16 E6 oncoprotein, Biochemistry 42, 3868-3873.
30. Dippold, W. G., Jay, G., DeLeo, A. B., Khoury, G., and Old, L. J. (1981) p53 transformation-related protein: Detection by monoclonal antibody in mouse and human cells, Proc. Natl. Acad. Sci. U.S.A. 78, 1695-1699.
31. Rotter, V., Abutbul, H., and Wolf, D. (1983) The presence of p53 transformation-related protein in Ab-MuLV transformed cells is required for their development into lethal tumors in mice, Int. J. Cancer 31, 315-320.
32. Thomas, M., Massimi, P., Navarro, C., Borg, J. P., and Banks, L. (2005) The hScrib/Dlg apico-basal control complex is differentially targeted by HPV-16 and HPV-18 E6 proteins, Oncogene 24, 6222-6230.
33. Woods, D. F., Wu, J. W., and Bryant, P. J. (1997) Localization of proteins to the apico-lateral junctions of Drosophila epithelia, Dev. Genet. 20, 111-118.