A new protocol for quantifying CD34+ Cells in peripheral blood of patients with cardiovascular disease

Texas Heart Institute Journal

Volumn 33, Issue 4, 2006, Pages 427-429

  Kikuchi Taura, Akie ^a   Soma, Toshihiro ^a   Matsuyama, Tomohiro ^b   Stern, David M ^c   Taguchi, Akihiko ^d  

^a MINAMI OSAKA GENERAL HOSPITAL   (Japan)

^b HYOGO COLLEGE OF MEDICINE   (Japan)

^c UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^d NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER   (Japan)

Author keywords

Antigens, CD34 analysis; Blood cell count; Cardiovascular diseases; CD34 positive cells; Cell sorter, fluorescence activated; Coefficients of variation; Endothelium, vascular cytology; Stem cells

Indexed keywords

ACETYLSALICYLIC ACID; ANTIHYPERTENSIVE AGENT; CALCIUM CHANNEL BLOCKING AGENT; DIPEPTIDYL CARBOXYPEPTIDASE INHIBITOR; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE INHIBITOR; SULFONYLUREA;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; BONE MARROW; CARDIOVASCULAR DISEASE; CARDIOVASCULAR RISK; CD34 SELECTION; CELL ASSAY; CELL COUNT; CELL FRACTIONATION; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; FEMALE; HIGH RISK PATIENT; HUMAN; MALE; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SCREENING TEST;

AGED; AGED, 80 AND OVER; ANTIGENS, CD34; CEREBRAL INFARCTION; CLINICAL PROTOCOLS; FEMALE; HEMATOPOIETIC STEM CELLS; HUMANS; LEUKOCYTE COUNT; MALE; MIDDLE AGED; MYOCARDIAL INFARCTION; REPRODUCIBILITY OF RESULTS; RISK FACTORS;

EID: 33846227512     PISSN: 07302347     EISSN: 15266702     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science 1997;275:964-7.
2. Majka M, Janowska-Wieczorek A, Ratajczak J, Ehrenman K, Pietrzkowski Z, Kowalska MA, et al. Numerous growth factors, cytokines, and chemokines are secreted by human CD34(+) cells, myeloblasts, erythroblasts, and megakaryoblasts and regulate normal hematopoiesis in an autocrine/paracrine manner. Blood 2001;97:3075-85.
3. Taguchi A, Ohtani M, Soma T, Watanabe M, Kinosita N. Therapeutic angiogenesis by autologous bone-marrow transplantation in a general hospital setting. Eur J Vasc Endovasc Surg 2003;25:276-8.
4. Hamano K, Nishida M, Hirata K, Mikamo A, Li TS, Harada M, et al. Local implantation of autologous bone marrow cells for therapeutic angiogenesis in patients with ischemic heart disease: clinical trial and preliminary results. Jpn Circ J 2001;65:845-7.
5. Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA, Finkel T. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med 2003;348:593-600.
6. Taguchi A, Matsuyama T, Moriwaki H, Hayashi T, Hayashida K, Nagatsuka K, et al. Circulating CD34-positive cells provide an index of cerebrovascular function. Circulation 2004;109:2972-5.
7. Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 1996;5:213-26.
8. Sutherland DR, Keeney M, Gratama JW. Enumeration of CD34+ hematopoietic stem and progenitor cells. In: Robinson JP, Darzynkiewicz Z, Dobrucki J, Hyun WC, Nolan JP, Orfao A, Rabinovitch PS, editors. Current protocols in cytometry. New York: John Wiley and Sons, Inc.; 2003. Unit 6.4, p. 1-23.