Chiral discrimination of α-amino acids by the DNA triplet GCA

Chemical Communications

Volumn , Issue 4, 2007, Pages 392-394

  Ravikumar, Maddula ^a   Prabhakar, Sripadi ^a   Vairamani, Mariappanadar ^a  

^a INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID DERIVATIVE; DNA;

ARTICLE; CHIRALITY; COLLISIONALLY ACTIVATED DISSOCIATION; DNA DETERMINATION; DNA SEQUENCE; ELECTROSPRAY MASS SPECTROMETRY; MEASUREMENT; MOLECULAR RECOGNITION; POLARIMETRY; TRINUCLEOTIDE REPEAT;

EID: 33846207469     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b613232d     Document Type: Article

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27. All the d and l- amino acids used were purchased from Aldrich (Steinheim, Germany), and trinucleotides were obtained from Sigma-Aldrich chemicals (Banglore, India) and used as received. Methanol (HPLC grade) was obtained from Merck (Mumbai, India). Stock solutions of trinucleotides (500 μM), amino acid (1 mM) sodium chloride (1 mM) were prepared in Milli Q water. These stock solutions are mixed in appropriate volumes and dilulted with methanol to obtain a final concentration of 50: 200 μM (1: 4) of trinucleotide and amino acid in 50: 50 water: methanol in the presence of 20 μM of NaCl. Sample solutions are introduced into the ESI source through an inbuilt infusion pump
28. All the experiments were performed using QSTAR XL (Applied Biosystems/MDS Sciex, Foster city, USA): operating capillary voltage -4.0 kV; declustering potential, 50 V. Nitrogen was used as the curtain and collision gas. The collision energy was set to be 12 eV
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